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ARS Home » Southeast Area » Raleigh, North Carolina » Soybean and Nitrogen Fixation Research » Research » Research Project #431886

Research Project: Developing Soybean Cultivars and Germplasm with Increased Protein Quantity and Quality using Diversity in Cultivated and Wild Soybeans

Location: Soybean and Nitrogen Fixation Research

Project Number: 6070-21220-069-001-R
Project Type: Reimbursable Cooperative Agreement

Start Date: Oct 1, 2016
End Date: Sep 30, 2018

Objective:
Glycine (G.) Max Objectives: 1. Develop soybean cultivar and germplasm with 37-38% seed protein without reducing seed yield and seed oil by tapping into genetically diverse high yielding G. max breeding lines. 2. Determine environmental stability of genetically diverse Glycine max high protein sources to be used in breeding. 3. Confirm/characterize known major protein quantitative trait loci (QTL), refine markers to be used in breeding and evaluate potential yield drag associated with major QTL. 4. Identify novel QTL to break/minimize the negative relation of seed protein with seed oil and seed yield. 5. Genotype high-protein high-yielding breeding lines developed by the group using BARCSoySNP6K chips. Glycine soja Objectives 1. Identify/confirm Glycine soja accessions with environmentally stable high protein and high sulfur amino acid contents. 2. Map high protein and high sulfur amino acid alleles in Glycine soja and determine their impact on seed yield. 3. Develop genetically diverse adapted germplasm with 37- 38% seed protein and good seed yield seed oil contents from G. max x G. soja crosses.

Approach:
Classical and marker assisted breeding will be used to develop soybean germplasm with high protein. Molecular mapping and genomics approaches will be used to map new genes controlling seed protein content. We will develop markers for marker assisted breeding. Genetic diversity in both Glycine (G.) max and wild soybean will be used for enhancing the genetic bases of high protein and high yielding soybean lines to be developed.