Location: Toxicology & Mycotoxin Research
Project Number: 6040-42000-013-000-D
Project Type: In-House Appropriated
Start Date: Feb 8, 2016
End Date: Jun 20, 2018
Objective 1. Compare the in vivo dose-response of fumonisin B1 to grain naturally contaminated with fumonisins or fumonisin-producing fungi. Objective 2. Determine the interactions between fumonisin exposure and dietary factors on fetal and postnatal development using animal models to provide basic information for ongoing translational human studies. Subobjective 2a. FBs as a risk factor for NTD with focus on how folates affect FB-dependent NTD induction in mice. Subobjective 2b. FBs and growth inhibition. Objective 3. Determine the efficacy of cooking methods to detoxify mycotoxins in co-contaminated corn using an in vivo rodent bioassay approach incorporating biomarkers.
1. Compare the dose-response for fumonisin-specific kidney effects in rats fed fumonisin B1 or fumonisin mixtures. 2. Compare dose-response in mouse strains sensitive (LM/Bc) and insensitive (SWV) to neural tube defect induction by fumonisin B1. Compare dose-response for neural tube defect induction and selected gene expressions in fumonisin B1-exposed mice fed folate deficient or folate sufficient diets. Compare neonatal growth rates in offspring of mice fed diets containing fumonisins. 3. Determine the efficacy of alkaline cooking (nixtamalization) to detoxify corn contaminated with aflatoxin alone or co-contaminated with aflatoxin and fumonisin using a rat feeding bioassay.