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ARS Home » Northeast Area » Orient Point, New York » Plum Island Animal Disease Center » Foreign Animal Disease Research » Research » Research Project #429661

Research Project: Ankara Virus Vector Assessment: Development of Viral-Vectored Vaccines for African Swine Fever Virus

Location: Foreign Animal Disease Research

Project Number: 8064-32000-060-07-S
Project Type: Non-Assistance Cooperative Agreement

Start Date: Jul 1, 2015
End Date: Feb 28, 2019

Objective:
This collaborative research agreement seeks to explore the efficacy of Ankara Virus (APV) as a vector for expressing African Swine Fever Virus (ASFV). Specific objectives include: 1. Construct APV vectors co-expressing specific ASFV genes. 2. Evaluate the protective effects of the mutated APV vectors.

Approach:
1. ARS PIADC will identify and provide selected recombinant African Swine Fever Virus (ASFV) genes which have known immunogenetic properties to the University of Wisconsin (UW). UW will manipulate the Ankara Virus (APV), introducing the recombinant ASFV genes, resulting in a mutated virus which, when replicated will express protein encoded by the ASFV genes. 2. Resulting mutated viruses will be tested in swine in vivo by ARS PIADC. Challenge studies against, and the presence of ASFV-related signed and mortality rates will be assessed. Upon successful trials utilizing homologuous virus, further studies will be conducted utilzing additional strains.