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ARS Home » Midwest Area » West Lafayette, Indiana » Crop Production and Pest Control Research » Research » Research Project #424784

Research Project: Identification, Characterization, and Deployment of Genes Important during Seed Development in Legumes

Location: Crop Production and Pest Control Research

Project Number: 5020-21000-007-000-D
Project Type: In-House Appropriated

Start Date: May 28, 2013
End Date: May 8, 2018

1) Identify genetic loci which contribute positive alleles to seed yield and quality traits and which control variation in seed quality, especially seed oil, protein, carbohydrate content, and fatty acid composition of legume seeds; develop strategies to facilitate effective transfer of useful genes into cultivated legumes; 1a) Mapping and characterizing mutant alleles that contribute to variation in seed fatty acid composition; 1b) Discovery of the molecular identity of genes that influence overall protein or oil content by forward genetics approaches. 2) Determine epigenetic patterns in soybean seeds during development, assess relationships between chromatin state and gene expression, and develop approaches to modify gene expression in developing seeds; 2a) Genome-wide profiling of epigenetic state and gene expression in developing soybean seeds by chromatin immunoprecipitation followed by sequencing (ChIP-seq) and RNA sequence-based expression profiling (RNA-seq). 3) Evaluate the outcrossing and diversity of Phytophthora sojae populations and host plant interactions. 4) Evaluate the population dynamics of Fusarium virguliforme. 5) Organize, manage, and conduct the Northern Uniform Soybean Tests.

The project uses molecular biological approaches to study the regulation of genes that are required for soybean seed development. Biochemical approaches are used to study the interactions and function of gene products that are important for the accumulation of storage proteins or fatty acids in seeds. Molecular and genetic tools are used to define genes and genetic intervals that contribute to seed protein and oil content in mutant plants.