Project Number: 8080-21000-022-04-R
Project Type: Reimbursable Cooperative Agreement
Start Date: Mar 1, 2013
End Date: Dec 1, 2013
The objectives of this project are: 1) identify potential effector genes in P. expansum that play a role in pathogenicity; and 2) identify QTLs for disease resistance in apple using a segrating mapping population of Malus sieversii x 'Royal Gala' (GMAL4593).
Comparative secretome analysis and BLAST sequence similarity searches will be used to identify putative effectors in the sequenced genome of P. expansum. Transcriptomic analysis using Illumina technology will be used to examine differential gene expression in resistant and susceptible apple genotypes of M. sieversii in response to P. expansum. QTL analysis will be performed independently and combined for both parents in the GMAL4593 population to identify loci affecting P. expansum. Marker density in the QTLs will be increased using additional SNPs and DIPs. Identification of candidate resistance genes located in the QTL will be done by examining the sequenced genome of 'Golden Delicious'.