1a. Objectives (from AD-416):
Evaluating drought tolerance, heat tolerance, salinity tolerance and winter hardiness of pea and faba bean germplasm.
1b. Approach (from AD-416):
Two thousand accessions of pea and 300 accessions of faba bean germplasm available from WRPIS will be grow out in open field in areas subjected to the respective abiotic stresses for evaluation. Established field practice for land preparation, planting and cultivation will be carried out for the experiments with or without replications. For drought resistance and heat tolerance, screening will be conducted in Yunnan province. For salinity tolerance and winter hardiness, screening will be conducted during winter in Qingdao and Beijing. The response to the stress of each accession will be scored on the scale of 1 to 3: 1) good tolerance; 2) intermediate tolerance and 3) no tolerance. Select the accessions with the highest tolerance to make crosses and produce segregating population for further studies such as QTL mapping and breeding.
3. Progress Report:
This progress contributes to the Objective 2 of the parental project: “Conduct genetic characterizations and phenotypic evaluations of genetic resources of the preceding crops and related wild species for priority genetic and agronomic traits”. In October 2012, 1,294 pea and 286 faba bean accessions provided by WRPIS were planted (single row microplots with ten seeds per accession) in Qingdao, China. Although the lowest temperature recorded was only -10° C in January 2013 at the experimental site, a good differentiation of the germplasm was achieved, indicating that Qiangdao is an ideal site for this experiment. Twenty-four (~8%) faba bean and 617 pea (~48%) accessions had one or more plants that survived through the winter in the field. The final result will be entered into the NPGS’ GRIN (genetic resource information network) database, which is accessible to everyone in the global research community. The first year result was presented as a poster at the First Legume Society Conference, May 9-11, 2013 in Serbia.