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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Hard Winter Wheat Genetics Research » Research » Research Project #421027


Location: Hard Winter Wheat Genetics Research

2013 Annual Report

1a. Objectives (from AD-416):
Extensively phenotype and genotype diverse and designed germplasm pools to mine and deploy alleles that improve barley and wheat adaptation and performance under biotic and abiotic stress, and couple these activities with the training of a new generation of plant breeders

1b. Approach (from AD-416):
Develop and implement large- and small-scale SNP genotyping platforms for barley and wheat breeding programs, and test novel sequence-based genotyping platforms. Provide replicated rust resistance phenotypic data on multiple sets of hard winter wheat lines grown in the field and greenhouse. Integrate the genotypic and phenotypic information into GRIN, GrainGenes, and Gramene databases and develop web-based tools to help breeders use this information.

3. Progress Report:
Approximately 2100 unadapted lines from the Triticeae Coordinated Agricultural Project (TCAP) winter wheat association mapping panel were rated for growth stage, leaf rust severity, and infection type twice at Castroville, TX, and once at Manhattan, KS. A supplemental set of unadapted lines was also rated at Hutchinson, KS in 2013. Two additional elite Winter Wheat Association Mapping panels were rated for growth stage, leaf rust severity, and infection type at Castroville, TX and Manhattan, KS. These data are ready for analysis. Gene pyramiding of shortened versions of stem rust resistance genes Sr22, Sr26, Sr35 and Sr57 has been completed in a cultivar 'Duster' background. First crosses have been made to ten commercial hard red or hard white winter wheat varieties to transfer the pyramid to additional backgrounds. The race shift in the wheat stripe rust pathogen in the Great Plains in 2012 to virulence on TAM111 was verified with adult plant tests. In collaboration with Texas A&M researchers, the putatively affected resistance gene in TAM111 was mapped. A paper is in final revision prior to submission.

4. Accomplishments