Location: Sunflower and Plant Biology Research2013 Annual Report
1a. Objectives (from AD-416):
The objective of this project is to characterize the genetic inheritance of partial resistance to Sclerotinia sclerotiorum in pea (Pisum sativum L.) and exploit this resistance in the development of improved varieties. White mold, caused by S. sclerotiorum (Lib.) de Bary, is an important fungal pathogen of many crop plants and can cause significant crop loss to peas when environmental conditions are suitable. Pea germplasm demonstrating partial resistance has recently been identified and will be incorporated in improved agronomically adapted cultivars. The resistant sources show three distinct mechanisms of resistance and these mechanisms will be pyramided in single adapted backgrounds in an effort to extend the durability of resistance.
1b. Approach (from AD-416):
A classical breeding and genetic approach will be used to study the inheritance of partial resistance to S. sclerotiorum in pea and develop improved cultivars. Four accessions of pea germplasm have been identified representing three distinct mechanisms of resistance. These accessions have been hybridized with adapted germplasm to develop large mapping populations. The adapted parents used in this project are currently being characterized in complementary mapping projects. Common parents among the populations allow maps generated in different populations to be combined readily based on common genetic markers. Recombinant inbred line populations will be developed using single seed descent to the F7. Initial resistance data generated based on F3 and F4 families will be mapped using F2 genotypes. Individual replicate plants will be inoculated at 15 days of age with agar plugs containing mycelium of S. sclerotiorum and placed in a humidity chamber for 3 days, at which time the white mold lesions will be measured. Plants will then be placed in a growth chamber at 25ºC for one weeks and individual plants will be scored for survival and nodal resistance (prevention of lesion movement through a node). Genetic maps will be developed using JoinMap 4.0, MapManager and MapMaker software packages and QTL data will be mapped using QTL Cartographer for Windows v. 2.5.
3. Progress Report:
This project was initiated on June 1, 2009, research is ongoing, and the overall objective is the identification of genetic factors (QTL) controlling partial resistance to white mold in pea. A Master’s level student was hired in the fall of 2009 to oversee and accomplish the tasks of this project and he completed his project and graduated in the Spring of 2012 from NDSU. The previous project resulted in a genetic linkage map based on F2 DNA from the cross, Lifter/PI240515 (PRIL-17) with 78 markers on nine linkage groups (LG) spanning 734 cM. Two quantitative trait loci (QTL) were identified based on phenotypic data from F2:3 and F3:4 families. A single QTL on LGIII explained 34.1% of the phenotypic variation for lesion expansion interval, while a second QTL on LGII(b) explained 2.5% of the phenotypic variation for nodal transmission inhibition. Several areas were identified for potential improvement in phenotypic evaluation. The F2 populations were advanced to the F7; however, due to severe rain events the seed increase of the populations was lost. The main progress on this project has been to regenerate those lines that were lost and to establish a complete set of recombinant inbred lines that can be used for future studies.