Location: Subtropical Plant Pathology Research
Project Number: 6618-22000-034-00-D
Project Type: In-House Appropriated
Start Date: May 31, 2007
End Date: May 8, 2012
1. Characterize ecology, biology, epidemiology, genetics and host interactions of domestic, exotic, newly emergent and re-emerging pathogens. 2. Develop/refine rapid, sensitive reliable detection/sampling methods for pathogens. 3. Develop or improve comprehensive integrated disease management strategies. a. Assess the impact of a wide array of control/mitigation/therapeutic strategies on Huanglongbing (HLB) and the Asian citrus psyllid epidemiology to evalute optiumu means of disease control.
The overall approach is to thoroughly characterize new exotic and emerging plant pathogens at multiple levels: epidemiologically epidemics will be followed and modeled by traditional and newer stochastic methods at the regional, and plantation levels, biologically the pathosystems will be characterized at the level of host-pathogen-vector interaction, as well as at the cellular, molecular and/or biochemical levels. New pathogens will be identified and characterized by molecular biological and traditional cultural methods. Recombinant DNA and genomics technologies will be applied to study host/pathogen interactions and to investigate virulence differences between strains of a pathogen. New CTV genotypes will be identified by cloning products obtained by PCR and degenerate primers and also by hybridization to a sequencing microarray. Primers for PCR diagnostics will be devised from novel CTV genotypes. An immunocapture-based PCR protocol will be developed for CTLV for assessment of genetic variability of CTLV populations from the US and from international locations.