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ARS Home » Pacific West Area » Pullman, Washington » Animal Disease Research » People & Locations » Lindsay Fry
Lindsay Fry
Animal Disease Research
Veterinary Medical Officer

Phone: (509) 335-3882
Fax: (509) 335-8328

3065 ADBF, WASHINGTON STATE UNIV
PULLMAN, WA 991646630

(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)

Projects
Control Strategies for Theileriosis
In-House Appropriated (D)
  Accession Number: 441269
Identifying Effective Immune Responses and Vaccine Development for Bovine Anaplasmosis
In-House Appropriated (D)
  Accession Number: 441274
Understanding Host-Bacterial Interactions to Mitigate Disease in Small Ruminants
In-House Appropriated (D)
  Accession Number: 443841
Collaborative Animal Research between Washington State University and ARS-ADRU
Non-Funded Cooperative Agreement (N)
  Accession Number: 442989
Collaborative Animal Research between Regents of the University of Idaho and the ARS - Animal Disease Research Unit
Non-Funded Cooperative Agreement (N)
  Accession Number: 442065
Development of Control Strategies for Theileria orientalis
Non-Assistance Cooperative Agreement (S)
  Accession Number: 442458
Development of Novel Preventive, Diagnostic, and Treatment Strategies for Theileriosis in Horses and Cattle
Non-Assistance Cooperative Agreement (S)
  Accession Number: 434449

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
A U.S. isolate of theileria orientalis ikeda is not transstadially transmitted to cattle by rhipicephalus microplus Reprint Icon - (Peer Reviewed Journal)
Onzere, C.K., Herndon, D.R., Hassan, A., Oyen, K.J., Poh, K.C., Scoles, G.A., Fry, L.M. 2023. A U.S. isolate of theileria orientalis ikeda is not transstadially transmitted to cattle by rhipicephalus microplus. Pathogens. 12(4):559. https://doi.org/10.3390/pathogens12040559.
Tulathromycin and diclazuril lack in vivo efficacy against Theileria haneyi, but tulathromycin is not associated with adverse clinical effects in six treated adult horses Reprint Icon - (Peer Reviewed Journal)
Onzere, C.K., Hulbert, M., Sears, K.P., Williams, L.B., Fry, L.M. 2023. Tulathromycin and diclazuril lack in vivo efficacy against Theileria haneyi, but tulathromycin is not associated with adverse clinical effects in six treated adult horses. Pathogens. 12(3),453. https://doi.org/10.3390/pathogens12030453.
Comparative analysis of the specificity of monoclonal antibodies developed against the bottlenose dolphin, Tursiops truncatus, TNF-a, IL1-ß, IL-6, IL-8, IL-10 with monoclonal antibodies made against ovine IFN-y bovine IL-17A Reprint Icon - (Peer Reviewed Journal)
Sacco, R.E., Elnaggar, M.M., Abdellrazeq, G.S., Fry, L.M., Hulubei, V., Davis, W.C., Harsla, T., Mucci, M.L. 2022. Comparative analysis of the specificity of monoclonal antibodies developed against the bottlenose dolphin, Tursiops truncatus, TNF-a, IL1-ß, IL-6, IL-8, IL-10 with monoclonal antibodies made against ovine IFN-y bovine IL-17A. Veterinary Immunology and Immunopathology. 250. Article 110456. https://doi.org/10.1016/j.vetimm.2022.110456.
Ex vivo platforms to study the primary and recall immune responses to intracellular mycobacterial pathogens and peptide-based vaccines Reprint Icon - (Peer Reviewed Journal)
Davis, W.C., Mahmoud, A.H., Abdellrazeq, G.S., Elnaggar, M.M., Dahl, J.L., Hulubei, V., Fry, L.M. 2022. Ex vivo platforms to study the primary and recall immune responses to intracellular mycobacterial pathogens and peptide-based vaccines. Frontiers in Veterinary Science. 9. Article 878347. https://doi.org/10.3389/fvets.2022.878347.
Theileria equi RAP-1a and RAP-1b proteins contain immunoreactive epitopes and are suitable candidates for vaccine and diagnostics development Reprint Icon - (Peer Reviewed Journal)
Onzere, C.K., Fry, L.M., Bishop, R.P., Da Silva, M., Madsen-Bouterse, S.A., Bastos, R.G., Knowles, D.P., Suarez, C.E. 2022. Theileria equi RAP-1a and RAP-1b proteins contain immunoreactive epitopes and are suitable candidates for vaccine and diagnostics development. International Journal for Parasitology. 52(6):385-397. https://doi.org/10.1016/j.ijpara.2022.01.004.
Clinical progression of Theileria haneyi in splenectomized horses reveals decreased virulence compared to Theileria equi Reprint Icon - (Peer Reviewed Journal)
Sears, K.P., Knowles, D.P., Fry, L.M. 2022. Clinical progression of Theileria haneyi in splenectomized horses reveals decreased virulence compared to Theileria equi. Pathogens. 11(2). Article 254. https://doi.org/10.3390/pathogens11020254.
Immunization against a conserved surface polysaccharide stimulates bovine antibodies with opsonic killing activity but does not protect against Babesia bovis challenge Reprint Icon - (Peer Reviewed Journal)
Taus, N.S., Cywes-Bently, C., Johnson, W.C., Pier, G.B., Fry, L.M., Mousel, M.R., Ueti, M.W. 2021. Immunization against a conserved surface polysaccharide stimulates bovine antibodies with opsonic killing activity but does not protect against Babesia bovis challenge. Pathogens. 10(12). Article 1598. https://doi.org/10.3390/pathogens10121598.
Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system Reprint Icon - (Peer Reviewed Journal)
Vimonish, R., Dinkel, K.D., Fry, L.M., Johnson, W.C., Capelli-Peixoto, J., Bastos, R.G., Scoles, G.A., Knowles, D.P., Madder, M., Chaka, G., Ueti, M.W. 2021. Isolation of infectious Theileria parva sporozoites secreted by infected Rhipicephalus appendiculatus ticks into an in vitro tick feeding system. Parasites & Vectors. 14. Article 616. https://doi.org/10.1186/s13071-021-05120-7.
Advances in understanding of the immune response to mycobacterial pathogens and vaccines through use of cattle and Mycobacterium avium subsp. paratuberculosis as a prototypic mycobacterial pathogen Reprint Icon - (Peer Reviewed Journal)
Davis, W.C., Abdellrazeq, G.S., Mahmoud, A.H., Park, K., Elnaggar, M.M., Donofrio, G., Hulubei, V., Fry, L.M. 2021. Advances in understanding of the immune response to mycobacterial pathogens and vaccines through use of cattle and Mycobacterium avium subsp. paratuberculosis as a prototypic mycobacterial pathogen. Vaccines. 9(10). Article 1085. https://doi.org/10.3390/vaccines9101085.
Flow cytometric analysis of the cytotoxic T-cell recall response to Theileria parva in cattle following vaccination by the infection and treatment method Reprint Icon - (Peer Reviewed Journal)
Elnaggar, M.M., Knowles, D.P., Davis, W., Fry, L.M. 2021. Flow cytometric analysis of the cytotoxic T-cell recall response to Theileria parva in cattle following vaccination by the infection and treatment method. Veterinary Sciences. 8(6). Article 114. https://doi.org/10.3390/vetsci8060114.
Theileria equi claudin like apicomplexan microneme protein contains neutralization-sensitive epitopes and interacts with components of the equine erythrocyte membrane skeleton Reprint Icon - (Peer Reviewed Journal)
Onzere, C.K., Fry, L.M., Bishop, R.P., Silva, M.G., Bastos, R.G., Knowles, D.P., Suarez, C.E. 2021. Theileria equi claudin like apicomplexan microneme protein contains neutralization-sensitive epitopes and interacts with components of the equine erythrocyte membrane skeleton. Scientific Reports. 11. Article 9301. https://doi.org/10.1038/s41598-021-88902-4.
A U.S. isolate of Theileria orientalis, Ikeda genotype, is transmitted to cattle by the invasive Asian longhorned tick, Haemaphysalis longicornis Reprint Icon - (Peer Reviewed Journal)
Dinkel, K.D., Herndon, D.R., Noh, S.M., Lahmers, K.K., Todd, M.S., Ueti, M.W., Scoles, G.A., Mason, K.L., Fry, L.M. 2021. A U.S. isolate of Theileria orientalis, Ikeda genotype, is transmitted to cattle by the invasive Asian longhorned tick, Haemaphysalis longicornis. Parasites & Vectors. 14. Article 157. https://doi.org/10.1186/s13071-021-04659-9.
Development of an indirect ELISA to detect equine antibodies to Theileira haneyi Reprint Icon - (Peer Reviewed Journal)
Bastos, R.G., Sears, K.P., Dinkel, K.D., Kappmeyer, L.S., Ueti, M.W., Knowles, D.P., Fry, L.M. 2021. Development of an indirect ELISA to detect equine antibodies to Theileira haneyi. Pathogens. 10(3). Article 270. https://doi.org/10.3390/pathogens10030270.
Imidocarb Dipropionate lacks efficacy against Theileria haneyi and fails to consistently clear Theileria equi in horses co-infected with T. haneyi Reprint Icon - (Peer Reviewed Journal)
Sears, K.P., Knowles, D.P., Dinkel, K.D., Mshelia, P.W., Onzere, C., Silva, M., Fry, L.M. 2020. Imidocarb Dipropionate lacks efficacy against Theileria haneyi and fails to consistently clear Theileria equi in horses co-infected with T. haneyi. Pathogens. 9(12). Article 1035. https://doi.org/10.3390/pathogens9121035.
Twenty years of equine piroplasmosis research: global distribution, molecular diagnosis, and phylogeny Reprint Icon - (Peer Reviewed Journal)
Tirosh-Levy, S., Gottlieb, Y., Fry, L.M., Knowles, D.P., Steinman, A. 2020. Twenty years of equine piroplasmosis research: global distribution, molecular diagnosis, and phylogeny. Pathogens. 9(11). Article 926. https://doi.org/10.3390/pathogens9110926.
Equid infective Theileria cluster in distinct 18S rRNA gene clades comprising multiple taxa with unusually broad mammalian host ranges Reprint Icon - (Peer Reviewed Journal)
Bishop, R.P., Kappmeyer, L.S., Onzere, C.K., Odongo, D.O., Githaka, N., Sears, K.P., Knowles, D.P., Fry, L.M. 2020. Equid infective Theileria cluster in distinct 18S rRNA gene clades comprising multiple taxa with unusually broad mammalian host ranges. Parasites & Vectors. 13. Article 261. https://doi.org/10.1186/s13071-020-04131-0.
Re-annotation of the Theileria parva genome refines 53% of the proteome and uncovers essential components of N-glycosylation, a conserved pathway in many organisms Reprint Icon - (Peer Reviewed Journal)
Tretina, K., Pelle, R., Orvis, J., Gotia, H.T., Ifeonu, O.O., Kumari, P., Palmateer, N.C., Iqbal, S.B., Fry, L.M., Nene, V.M., Daubenberger, C.A., Bishop, R.P., Silva, J.C. 2020. Re-annotation of the Theileria parva genome refines 53% of the proteome and uncovers essential components of N-glycosylation, a conserved pathway in many organisms. BMC Genomics. 21. https://doi.org/10.1186/s12864-020-6683-0.
Theileria parasites subvert E2F signaling to stimulate leukocyte proliferation Reprint Icon - (Peer Reviewed Journal)
Tretina, K., Haidar, M., Madsen-Bouterse, S.A., Sakura, T., Mfarrej, S., Fry, L.M., Chaussepied, M., Pain, A., Knowles, D.P., Nene, V.M., Ginsberg, D., Daubenberger, C.A., Bishop, R.P., Langsley, G., Silva, J.C. 2020. Theileria parasites subvert E2F signaling to stimulate leukocyte proliferation. Scientific Reports. 10. https://doi.org/10.1038/s41598-020-60939-x.
relA is Achilles’ heel for mycobacterial pathogens as demonstrated with deletion mutants in Mycobacterium avium subsp. paratuberculosis and mycobacterium bovis bacillus Calmette-Guérin (BCG) Reprint Icon - (Peer Reviewed Journal)
Abdellrazeq, G.S., Mahmoud, A.H., Kun-Taek, P., Fry, L.M., Elnaggar, M.M., Schneider, D.A., Hulubei, V., Davis, W.C. 2020. relA is Achilles’ heel for mycobacterial pathogens as demonstrated with deletion mutants in Mycobacterium avium subsp. paratuberculosis and mycobacterium bovis bacillus Calmette-Guérin (BCG). Tuberculosis. 120. https://doi.org/10.1016/j.tube.2020.101904.
Simultaneous cognate epitope recognition by bovine CD4 and CD8 T cells is essential for primary expansion of antigen-specific cytotoxic T-cells following ex vivo stimulation with a candidate Mycobacterium avium supsp. Reprint Icon - (Peer Reviewed Journal)
Abdellrazeq, G.S., Fry, L.M., Elnaggar, M.M., Bannantine, J.P., Schneider, D.A., Chamberlain, W.M., Mahmoud, A.H.A., Park, K.T., Hulubei, V., Davis, W.C. 2020. Simultaneous cognate epitope recognition by bovine CD4 and CD8 T cells is essential for primary expansion of antigen-specific cytotoxic T-cells following ex vivo stimulation with a candidate Mycobacterium avium supsp. paratubeculosis peptide vaccine. Vaccine. 38(8):2016-2025. https://doi.org/10.1016/j.vaccine.2019.12.052.
Capacity to elicit Cytotoxic CD8 T cell activity against Mycobacterium avium subsp. paratuberculosis is retained in a vaccine candidate 35 kD peptide modified for expression in mammalian cells Reprint Icon - (Peer Reviewed Journal)
Franceschi, V., Asmma, M.H., Abdellrazeq, G.S., Tebaldi, G., Macchi, F., Russo, L., Fry, L.M., Elnaggar, M.M., Bannantine, J.P., Park, K., Hulubei, V., Cavirani, S., Davis, W.C., Donofrio, G. 2019. Capacity to elicit Cytotoxic CD8 T cell activity against Mycobacterium avium subsp. paratuberculosis is retained in a vaccine candidate 35 kD peptide modified for expression in mammalian cells . Frontiers in Immunology. https://doi.org/10.3389/fimmu.2019.02859.
Changes in the molecular and functional phenotype of bovine monocytes during Theileria parva infection Reprint Icon - (Peer Reviewed Journal)
Bastos, R.G., Sears, K.P., Dinkel, K.D., Knowles, D.P., Fry, L.M. 2019. Changes in the molecular and functional phenotype of bovine monocytes during Theileria parva infection. Infection and Immunity. 87(12). https://doi.org/10.1128/IAI.00703-19.
A review of recent research on Theileria parva: Implications for the infection and treatment vaccination method for control of East Coast fever Reprint Icon - (Peer Reviewed Journal)
Bishop, R.P., Odongo, D., Ahmed, J., Mwamuye, M., Fry, L.M., Knowles, D.P., Nanteza, A., Lubega, G., Gwakisa, P., Clausen, P., Obara, I. 2020. A review of recent research on Theileria parva: Implications for the infection and treatment vaccination method for control of East Coast fever. Transboundary and Emerging Diseases. 67(51):56-67. https://doi.org/10.1111/tbed.13325.
Molecular and antigenic properties of mammalian cellexpressed Theileria parva antigen Tp9 Reprint Icon - (Peer Reviewed Journal)
Bastos, R.G., Franceschi, V., Tebaldi, G., Connelley, T., Morrison, I.W., Knowles, D.W., Donofrio, G., Fry, L.M. 2019. Molecular and antigenic properties of mammalian cellexpressed Theileria parva antigen Tp9. Frontiers in Immunology. 10. https://doi.org/10.3389/fimmu.2019.00897.
A recombinant bovine herpesvirus-4 vectored vaccine delivered via intranasal nebulization elicits viral neutralizing antibody titers in cattle Reprint Icon - (Peer Reviewed Journal)
Williams, L.B., Fry, L.M., Herndon, D.R., Franceschi, V., Schneider, D.A., Donofrio, G., Knowles, D.P. 2019. A recombinant bovine herpesvirus-4 vectored vaccine delivered via intranasal nebulization elicits viral neutralizing antibody titers in cattle. PLoS One. 14(4):e0215605. https://doi.org/10.1371/journal.pone.0215605.
Pattern of CD14, CD16, CD163 and CD172a expression on water buffalo (Bubalus bubalis) leukocytes Reprint Icon - (Peer Reviewed Journal)
Elnaggar, M.M., Grandoni, F., Abdellrazeq, G.S., Fry, L.M., El-Naggar, K., Hulubei, V., Buttazzoni, L., Khaliel, S.A., Torky, H.A., Davis, W.C. 2019. Pattern of CD14, CD16, CD163 and CD172a expression on water buffalo (Bubalus bubalis) leukocytes. Veterinary Immunology and Immunopathology. 211:1-5. https://doi.org/10.1016/j.vetimm.2019.03.010.
Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule Reprint Icon - (Peer Reviewed Journal)
Fry, L.M., Bastos, R.G., Stone, B.C., Williams, L.B., Knowles, D.P., Murphy, S.C. 2019. Gene gun DNA immunization of cattle induces humoral and CD4 T-cell-mediated immune responses against the Theileria parva polymorphic immunodominant molecule. Vaccine. 37(12):1546-1553. https://doi.org/10.1016/j.vaccine.2019.02.009.
Characterization of alpha beta and gamma delta T cell subsets expressing IL-17A in ruminants and swine Reprint Icon - (Peer Reviewed Journal)
Elnaggar, M.M., Abdellrazeq, G.S., Dassanayake, R.P., Fry, L.M., Hulubei, V., Davis, W.C. 2018. Characterization of alpha beta and gamma delta T cell subsets expressing IL-17A in ruminants and swine. Developmental and Comparative Immunology. 85:115-124. https://doi.org/10.1016/j.dci.2018.04.003.
Discovery of a novel species Theileria haneyi n. sp. infective to equids highlights exceptional genomic diversity within the genus Theileria: implications for apicomplexan parasite surveillance Reprint Icon - (Peer Reviewed Journal)
Knowles, D.P., Kappmeyer, L.S., Haney, D., Herndon, D.R., Fry, L.M., Munro, J.B., Sears, K., Ueti, M.W., Wise, L.N., Silva, M., Schneider, D.A., Grause, J., White, S.N., Trentina, K., Bishop, R., Odongo, D.O., Pelzel-McCluske, A.M., Scoles, G.A., Mealey, R.H., Silva, J.C. 2018. Discovery of a novel species Theileria haneyi n. sp. infective to equids highlights exceptional genomic diversity within the genus Theileria: implications for apicomplexan parasite surveillance. International Journal for Parasitology. 48(9-10):127-137. https://doi.org/10.1016/j.ijpara.2018.03.010.
Assessment and optimization of theileria parva sporozoite full-length p67 antigen expression in mammalian cells - (Peer Reviewed Journal)
Tebaldi, G., Williams, L.B., Verna, A.E., Macchi, F., Franceshi, V., Fry, L.M., Knowles Jr, D.P., Donofrio, G. 2017. Assessment and optimization of theileria parva sporozoite full-length p67 antigen expression in mammalian cells. PLOS Neglected Tropical Diseases. https://doi.org/10.1371/journal.pntd.0005803.
Characterization of leukocyte subsets in buffalo (Bubalus bubalis) with cross-reactive monoclonal antibodies specific for bovine MHC class I and class II molecules and leukocyte differentiation molecules - (Peer Reviewed Journal)
Davis, W.C., Grandoni, F., Mahmoud, E.M., Abdellrazeq, G.S., Fry, L.M., Mack, V., Marchitelli, C., Barile, V.L. 2017. Characterization of leukocyte subsets in buffalo (Bubalus bubalis) with cross-reactive monoclonal antibodies specific for bovine MHC class I and class II molecules and leukocyte differentiation molecules. Developmental and Comparative Immunology. 74:101-109.
Phenotype and function of CD209+ bovine blood dendritic cells, monocyte-derived-dendritic cells and monocyte-derived macrophages - (Peer Reviewed Journal)
Park, K., Elnagger, M., Abdellrazeq, G., Bannantine, J.P., Mack, V., Fry, L.M., Davis, W.C. 2016. Phenotype and function of CD209+ bovine blood dendritic cells, monocyte-derived-dendritic cells and monocyte-derived macrophages. PLoS One. 11(10):e0165247. doi:10.14371/journal.pone.0165247.org/.
East coast fever caused by Theileria parva is characterized by macrophage activation associated with vasculitis and respiratory failure - (Peer Reviewed Journal)
Fry, L.M., Schneider, D.A., Frevert, C.W., Nelson, D.D., Morrison, I., Knowles, D.P. 2016. East coast fever caused by Theileria parva is characterized by macrophage activation associated with vasculitis and respiratory failure. PLoS One. doi:10.1371/journal.pone.0156004.
Characterization and use of new monoclonal antibodies to CD11c,CD14, and CD163 to analyze the phenotypic complexity of ruminantmonocyte subsets - (Peer Reviewed Journal)
Elnaggar, M.M., Abdellrazeq, G.S., Mack, V., Fry, L.M., Davis, W.C., Park, T. 2016. Characterization and use of new monoclonal antibodies to CD11c,CD14, and CD163 to analyze the phenotypic complexity of ruminantmonocyte subsets. Veterinary Immunology and Immunopathology. 178:57-63.
Immunization-induced anaplasma marginale-specific T lymphocyte reponses impaired by A. marginale infection are restored after eliminating the infection with tetracycline - (Peer Reviewed Journal)
Turse, J.E., Scoles, G.A., Deringer, J.R., Fry, L.M., Brown, W.C. 2014. Immunization-induced anaplasma marginale-specific T lymphocyte reponses impaired by A. marginale infection are restored after eliminating the infection with tetracycline . Clinical and Vaccine Immunology. 21(9):1369-75.