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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » People & Locations » Roger Thilmony

Roger Thilmony
Crop Improvement and Genetics Research
Research Molecular Biologist

Phone: (510) 559-5761
Fax: (510) 559-5818
Room 2111

USDA, ARS, PWA, WRRC-CIU
800 BUCHANAN STREET
ALBANY , CA 94710
Projects
Molecular Genetic Tools Advancing the Application of Biotechnology for Crop Improvement
In-House Appropriated (D)
  Accession Number: 434430
Expanding the Breeder's Toolbox for Perennial Grasses
Interagency Reimbursable Agreement (I)
  Accession Number: 433064
Development of a Transgene Biocontainment System for Switchgrass
Interagency Reimbursable Agreement (I)
  Accession Number: 433694
Developing Abiotic Stress Tolerant Wheat Using Gene Editing
Trust Fund Cooperative Agreement (T)
  Accession Number: 434920

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
A versatile and robust Agrobacterium-based gene stacking system generates high quality transgenic Arabidopsis plants -
Collier, R., Thomson, J.G., Thilmony, R.L. 2018. A versatile and robust Agrobacterium-based gene stacking system generates high quality transgenic Arabidopsis plants. Plant Journal. 95(4):573-583. https://doi.org/10.1111/tpj.13992.
Salt tolerance of two perennial grass Brachypodium sylvaticum accessions -
Sade, N., del Mar Rubio-Wilhelmi, M., Ke, X., Brotman, Y., Wright, M., Khan, I., De Souza, W., Bassil, E., Tobias, C.M., Thilmony, R.L., Vogel, J.P., Blumwald, E. 2018. Salt tolerance of two perennial grass Brachypodium sylvaticum accessions. Plant Molecular Biology. 96(3):305-314. https://doi.org/10.1007/s11103-017-0696-3.
Escherichia coli O157:H7 converts plant-derived choline to glycine betaine for osmoprotection during pre- and post-harvest colonization of injured lettuce leaves -
Scott, R.A., Thilmony, R.L., Harden, L.A., Zhou, Y., Brandl, M. 2017. Escherichia coli O157:H7 converts plant-derived choline to glycine betaine for osmoprotection during pre- and post-harvest colonization of injured lettuce leaves. Frontiers in Microbiology. 8:2436. https://doi.org/10.3389/fmicb.2017.02436.
Measurement of Transgene Copy Number in Potatoes Using Droplet Digital PCR -
Mc Cue, K.F., Collier, R.A., Gardner, E.B., Thilmony, R.L. 2018. Measurement of Transgene Copy Number in Potatoes Using Droplet Digital PCR. Meeting Abstract. 95(3):208-229. https://doi.org/10.1007/s12230-018-9650-4.
Accurate measure of transgene copy number in crop plants using droplet digital PCR -
Collier, R.A., Xing, Y., Lin, J.W., Mc Cue, K.F., Blechl, A.E., Thomson, J.G., Thilmony, R.L., Dasgupta, K., Hernandez, B.T., Shao, M., Oliveira, M.L., Stover, E.W., Novak, E., Harmon, F.G., Rohozinski, D. 2017. Accurate measure of transgene copy number in crop plants using droplet digital PCR. Plant Journal. 9(5):1014-1025 doi: 10.1111/TPJ.13517.
Accurate measurement of transgene copy number in crop plants using droplet digital PCR -
Collier, R.A., Dasgupta, K., Xing, Y., Hernandez, B., Shao, M., Rohozinski, D., Kovak, E., Lin, J.W., De Oliveira, M., Stover, E.W., Mc Cue, K.F., Harmon, F.G., Blechl, A.E., Thomson, J.G., Thilmony, R.L. 2017. Accurate measurement of transgene copy number in crop plants using droplet digital PCR. Plant Journal. 90:1014-1025. https://doi.org/10.1111/tpj.13517.
Novel R2R3-MYB transcription factors from Prunus americana regulate differential patterns of anthocyanin accumulation in tobacco and citrus -
Dasgupta, K., Thilmony, R.L., Stover, E.W., Oliveira, M.L., Thomson, J.G. 2017. Novel R2R3-MYB transcription factors from Prunus americana regulate differential patterns of anthocyanin accumulation in tobacco and citrus. GM Crops & Food. 8:85-105. https://doi.org/10.1080/21645698.2016.1267897.
Novel R2R3-MYB transcription factors from Prunus Americana regulate differential patterns of anthocyanin accumulation in tobacco and citrus -
Dasgupta, K., Thilmony, R.L., Stover, E.W., Oliveira, M.L., Thomson, J.G. 2017. Novel R2R3-MYB transcription factors from Prunus Americana regulate differential patterns of anthocyanin accumulation in tobacco and citrus . GM Crops & Food. 8:85-105. doi: 10.1080/21645698.2016.1267897
PIECE 2.0: an update for the plant gene structure comparison and evolution database -
Wang, Y., Xu, L., Thilmony, R.L., You, F., Gu, Y.Q., Coleman-Derr, D.A. 2016. PIECE 2.0: an update for the plant gene structure comparison and evolution database. Nucleic Acids Research. 45(D1):1015-1020. https://doi.org/10.1093/nar/gkw935.
Draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659 -
Valdes, J., Collier, R.A., Wang, Y., Huo, N., Gu, Y.Q., Thilmony, R.L., Thomson, J.G. 2002. Draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659. Genome Announcements. 4:1-2.
Use of Agrobacterium rhizogenes strain 18r12v and paromomycin selection for transformation of Brachypodium distachyon and Brachypodium sylvaticum -
Collier, R.A., Bragg, J., Hernandez, B., Vogel, J., Thilmony, R.L. 2016. Use of Agrobacterium rhizogenes strain 18r12v and paromomycin selection for transformation of Brachypodium distachyon and Brachypodium sylvaticum. Frontiers in Plant Science. doi: 10.3389/fpls.2016.00716.
Small cyclic amphipathic peptides (SCAmpPs) genes in citrus provide promising tools for more effective tissue specific transgenic expression -
Belknap, W.R., Thomson, J.G., Thilmony, R.L., McCue, K.F., Hao, G., Stover, E.W. 2017. Small cyclic amphipathic peptides (SCAmpPs) genes in citrus provide promising tools for more effective tissue specific transgenic expression. Acta Horticulturae. 1172:85-90. https://doi.org/10.17660/ActaHortic.2017.1172.15.
Brachypodium seed - a potential model for studying grain development of cereal crops -
Wu, J., Thilmony, R.L., Gu, Y.Q. 2016. Brachypodium seed - a potential model for studying grain development of cereal crops. In:Vogel, J., editor. Genetics and Genomics of Brachypodium. Cham, Switzerland:Springer International Publishing. p. 219-243. doi: 10.1007/7397.2015.12.
The development of novel Blood and Cara cara like citrus varieties -
Thomson, J.G., Dasgupta, K., Thilmony, R.L. 2015. The development of novel Blood and Cara cara like citrus varieties. Citrograph. 6:3/65-69.
AIM: A comprehensive Arabidopsis Interactome Module database and related interologs in plants -
Wang, Y., Thilmony, R.L., Zhao, Y., Chen, G., Gu, Y.Q. 2014. AIM: A comprehensive Arabidopsis Interactome Module database and related interologs in plants. Database: The Journal of Biological Databases and Curation. doi: 10.1093/database/bau117.
Transgene autoexcision in switchgrass pollen mediated by the Bxb1 recombinase -
Somleva, M.N., Chang, A.X., Ryan, K.P., Thilmony, R.L., Peoples, O., Snell, K.D., Thomson, J.G. 2014. Transgene autoexcision in switchgrass pollen mediated by the Bxb1 recombinase. BioMed Central (BMC)Biotechnology. 14:79. doi: 10.1186/1472-6750-14-79.
Net Venn - An integrated network analysis web platform for gene lists -
Wang, Y., Thilmony, R.L., Gu, Y.Q. 2014. Net Venn - An integrated network analysis web platform for gene lists. Nucleic Acids Research. DOI: 10.1093/narlgku331.
The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon Reprint Icon -
Thilmony, R.L., Guttman, M.E., Lin, J.W., Blechl, A.E. 2014. The wheat HMW-glutenin 1Dy10 gene promoter controls endosperm expression in Brachypodium distachyon. GM Crops. 5:36-43.
Novel gene expression tools for rice biotechnology -
Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86 -
Hurkman II, W.J., Tanaka, C.K., Vensel, W.H., Thilmony, R.L., Altenbach, S.B. 2013. Comparative proteomic analysis of the effect of temperature and fertilizer on gliadin and glutenin accumulation in the developing endosperm and flour from Triticum aestivum L. cv. Butte 86. Proteome Science. doi:10.1186/1477-5956-11-8.
Screening promoters for Anthurium transformation using transient expression -
Matsumoto Brower, T.K., Keith, L.M., Cabos, R.Y.M., Suzuki, J.Y., Gonsalves, D., Thilmony, R.L. 2013. Screening promoters for Anthurium transformation using transient expression. Plant Cell Reports. 32:443-451.
PIECE: A database for plant gene structure comparison and evolution -
Wang, Y., You, F., Lazo, G.R., Luo, M., Thilmony, R.L., Gordon, S.P., Gu, Y.Q., Kianian, S. 2012. PIECE: A database for plant gene structure comparison and evolution. Nucleic Acids Research. D1159-D1166.
Generation and characterization of the Western Regional Research Center brachypodium T-DNA insertional mutant collection -
Bragg, J., Wu, J., Gordon, S.P., Guttman, M.E., Thilmony, R.L., Lazo, G.R., Gu, Y.Q., Vogel, J.P. 2012. Generation and characterization of the Western Regional Research Center brachypodium T-DNA insertional mutant collection. PLoS One. 7(9):e41916. doi:10.1371/journal.pone.0041916.
Bxb1 recombinase mediates site-specific deletions in transgenic wheat -
Blechl, A.E., Lin, J.W., Shao, M., Thilmony, R.L., Thomson, J.G. 2012. Bxb1 recombinase mediates site-specific deletions in transgenic wheat. Plant Molecular Biology Reporter. doi:10.1007/s11105-012-0454-2.
The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis Reprint Icon -
Thomson, J.G., Chan, R., Smith, J.D., Thilmony, R.L., Yau, Y., Wang, Y., Ow, D.W. 2012. The Bxb1 recombination system demonstrates heritable transmission of site-specific excision in Arabidopsis. BioMed Central (BMC)Biotechnology. 12:9 doi:10.1186/1472-6750-12-9.
Transformation of Lesquerella fendleri with the new binary vector pGPro4-35S -
Chen, G.Q., Thilmony, R.L., Lin, J.T. 2012. Transformation of Lesquerella fendleri with the new binary vector pGPro4-35S. Online Journal of Biological Sciences. 11(3)90-95. DOI: 10.3844/ojbsci.2011.90.95.
Novel sull binary vectors enable an inexpensive foliar selection method in Arabidopsis -
Thomson, J.G., Cook, M.A., Guttman, M.E., Smith, J.D., Thilmony, R.L. 2011. Novel sull binary vectors enable an inexpensive foliar selection method in Arabidopsis. BMC Research Notes. 4(44) Available: http://www.biomedcentral.com/1756-0500/4/44.
The phiC31 Recombinase Demonstrates Heritable Passage of Site-specific Genomic Excision in Arabidopsis -
Thomson, J.G., Chan, R., Thilmony, R.L., Ow, D.W. 2010. The phiC31 Recombinase Demonstrates Heritable Passage of Site-specific Genomic Excision in Arabidopsis. BioMed Central (BMC)Biotechnology. Available: doi:10.1186/1472-6750-10-17.
The effect of high temperature on cell structure and gluten protein accumulation in the endosperm of the developing wheat (Triticum aestivum L.) grain -
Hurkman Ii, W.J., Tanaka, C.K., Thilmony, R.L., Williams, T.G., Wood, D.F., Vensel, W.H., Dupont, F.M., Altenbach, S.B. 2010. The effect of high temperature on cell structure and gluten protein accumulation in the endosperm of the developing wheat (Triticum aestivum L.) grain. International Gluten Workshop. pp. 50-54.
ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome -
Thomson, J.G., Yuan-Yeu, Y., Blanvillain, R., Chiniquy, D., Thilmony, R.L., Ow, D.W. 2009. ParA resolvase catalyzes site-specific excision of DNA from the Arabidopsis genome. Transgenic Research. 18:237-248.
A Simple and Inexpensive Method for Sending Binary Vector Plasmid DNA by Mail -
Thilmony, R.L., Thomson, J.G. 2008. A simple and inexpensive method for sending binary vector plasmid dna by mail. Plant Biotechnology. 25:403-406.
A Novel Rice LRR-Receptor Kinase Promoter with Organ-Specific Expression -
RAR1, a Central Player in Plant Immunity, is Targeted by Pseudomonas syringae Effector AvrB -
Shang, Y., Li, X., Cui, H., He, P., Thilmony, R.L., Chintamanani, S., Zwiesler-Vollick, J., Gopalan, S., Tang, X., Zhou, J. 2006. Rar1, a central player in plant immunity, is targeted by pseudomonas syringae effector avrb. Proceedings of the National Academy of Sciences. PNAS vol. 103 no. 50 pg. 19200-19205
CONSTRUCTION AND CHARACTERIZATION OF TWO BAC LIBRARIES FROM BRACHYPODIUM DISTACHYPON, A NEW MODEL FOR GRASS GENOMICS -
Gu, Y., Lazo, G., Vogel, J., Coleman-Derr, D., Luo, M.C., Thilmony, R., Garvin, D., Anderson, O., Huo, N. 2006. Construction and characterization of two BAC libraries from Brachypodium distachyon, a new model for grass genomics. Genome. 49(9):1099-1108.
PGPRO 1, A NOVEL BINARY VECTOR FOR MONOCOT PROMOTER CHARACTERIZATION -
Thilmony, R.L., Guttman, M.E., Chiniquy, D., Blechl, A.E. 2006. Pgpro 1, a novel binary vector for monocot promoter characterization. Plant Molecular Biology Reporter. 24:1-13, March 2006
GENOME-WIDE TRANSCRIPTIONAL ANALYSIS OF THE ARABIDOPSIS THALIANA INTERACTION WITH THE PLANT PATHOGEN PSEUDOMONAS SYRINGAE PV. TOMATO DC3000 AND THE HUMAN PATHOGEN ESCHERICHIA COLI 0157:H7. -
Thilmony, R.L., Underwood, W., He, S. 2006. Genome-wide transcriptional analysis of the compatible arabidopsis thaliana-psuedomonas syringae pv. tomato dc3000 interaction. Plant Journal. 46(1):34-53.
A FAMLIY OF CONSERVED BACTERIAL EFFECTORS INHIBITS SALICYLIC ACID-MEDITATED BASAL IMMUNITY AND PROMOTES DISEASE NECROSIS IN PLANTS -
Debroy, S., Thilmony, R.L., Kwack, Y., Nomura, K., He, S. 2004. A famliy of conserved bacterial effectors inhibits salicylic acid-meditated basal immunity and promotes disease necrosis in plants. Proceedings of the National Academy of Sciences. 101: 9927-9932
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