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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Crop Improvement and Genetics Research » Research » Publications at this Location » Publication #329911

Research Project: Molecular Tools for Improved Crop Biotechnology

Location: Crop Improvement and Genetics Research

Title: Draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659

Author
item Valdes, Jose Franco - Universidad Autonoma De Nuevo Leon
item Collier, Ray
item Wang, Yi - University Of California
item Huo, Naxin - University Of California
item Gu, Yong
item Thilmony, Roger
item Thomson, James - Jim

Submitted to: Genome Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 6/6/2016
Publication Date: 4/1/2002
Citation: Valdes, J., Collier, R.A., Wang, Y., Huo, N., Gu, Y.Q., Thilmony, R.L., Thomson, J.G. 2002. Draft genome sequence of Agrobacterium rhizogenes strain NCPPB2659. Genome Announcements. 4:1-2.

Interpretive Summary: This research describes the DNA preparation, sequencing, alignment, verification and gene annotation of the genome for Agrobacterium rhizogenes strain NCPPB2659. The genome is composed of one linear chromosome, a circular chromosome and a virulence plasmid. The latter can be transferred to plants, causing their roots to be highly branched (“hairy roots”). Data obtained in this study was used to remove the major gene responsible for homologous recombination from the genome. The derivative strain was shown to to be useful for transforming both monocot and dicot plants.

Technical Abstract: This work reports the draft genome of Agrobacterium rhizogenes strain NCPPB2659 (also known as strain K599). The assembled genome contains 5,277,347 bp, and is composed of 1 circular chromosome, the Ri virulence plasmid, and 17 scaffolds pertaining to the linear chromosome. The wild type strain causes hairy root disease in dicot plants and has been used to make transgenic hairy root cultures and composite plants (nontransgenic shoots with transgenic roots). Disarmed variants of the strain have been used to produce stable transgenic monocot and dicot plants. Data obtained in this study was used to remove the recA gene, thus producing a derivative strain better able to maintain repetitive elements in vectors used for transgenesis.