|He, Sheng Yang|
Submitted to: Plant Journal
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/6/2006
Publication Date: 2/6/2006
Citation: Thilmony, R.L., Underwood, W., He, S. 2006. Genome-wide transcriptional analysis of the compatible arabidopsis thaliana-psuedomonas syringae pv. tomato dc3000 interaction. Plant Journal. 46(1):34-53. Interpretive Summary: Plant disease causes significant crop losses annually. Our understanding of how plant pathogens cause disease is limited. The goal of this research is to increase our understanding of the molecular mechanisms underlying plant susceptibility to bacterial infection. This research characterized how bacterial pathogen virulence systems alter host plant gene expression promoting disease.
Technical Abstract: Pseudomonas syringae pv. tomato DC3000 (Pst) is a virulent pathogen, which causes disease on tomato and Arabidopsis. The type III secretion system (TTSS) plays a key role in pathogenesis by translocating virulence effectors from the bacteria into the plant host cell, while the phytotoxin coronatine (COR) contributes to virulence and disease symptom development. Recent studies suggest that both the TTSS and and COR are involved in the suppression of host basal defenses. However, little is known about the interplay between the host gene expression changes associated with basal defenses and the virulence activities of the TTSS and COR during infection. In this study, we used the Affymetrix full genome chip to determine the Arabidopsis transcriptome associated with basal defense to Pst DC3000 hrp mutants and the human pathogenic bacterium Escherichia coli O157:H7. We then used Pst DC3000 virulence mutants to characterize Arabidopsis transcriptional responses to the action of hrp-regulated virulence factors (e.g., TTSS and COR) during bacterial infection. Additionally, we used bacterial fliC mutants to assess the role of the PAMP flagellin in induction of basal defense-associated transcriptional responses. In total, our global gene expression analysis identified more than 5000 Arabidopsis genes that are reproducibly regulated in response to bacterial pathogen inoculation.