Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 1/15/2009
Publication Date: 1/15/2009
Citation: Thilmony, R.L., Cook, M.A. 2009. Intragenic Tools for Improved Rice Biotechnology and Biosafety(abstract). Crop Improvement and Utilization Intragenics Workshop. p. 2.
Technical Abstract: We are developing strategies for the production of intragenic rice. Agrobacterium-mediated co-transformation with two independent transfer-DNAs on separate binary vectors is being examined. An intragenic binary vector with a rice OSB2 expression cassette flanked by rice-derived left and right border-like sequences was constructed. OSB2 expression activates anthocyanin accumulation providing a visible phenotype. The second binary vector contains antibiotic selection and scorable markers within the transfer-DNA, facilitating identification of transformed cells. Selectable marker-free intragenic rice plants that contain only rice-derived genes and expression control elements will be identified upon segregation of the introduced sequences. Progress implementing this strategy will be presented.