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ARS Home » Midwest Area » Ames, Iowa » National Animal Disease Center » Virus and Prion Research » People & Locations » Justin Greenlee

Justin Greenlee
Virus and Prion Research
Research Veterinary Medical Officer

Phone: (515) 337-7191
Fax: (515) 337-7149

P.O. BOX 70
1920 DAYTON AVE
AMES, IA 50010

(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)

Projects
Elucidating the Pathobiology and Transmission of Transmissible Spongiform Encephalopathies
In-House Appropriated (D)
  Accession Number: 440677
Assessing the Potential Transmissibility of Bovine and Cervid Prions with a Human Prion Protein-based Model
Non-Assistance Cooperative Agreement (S)
  Accession Number: 442757
Investigation of a PRNP Polymorphism on Susceptibility to Bovine Spongiform Encephalopathy (BSE)
Non-Assistance Cooperative Agreement (S)
  Accession Number: 437152
Development of a Transgenic Mouse that Expresses the Bovine Prion Polymorphism T231
Non-Assistance Cooperative Agreement (S)
  Accession Number: 437176

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
White-Tailed Deer are Susceptible to the Agent of Sheep Scrapie after Experimental Oronasal Exposure - (Peer Reviewed Journal)
The effect on prion incubation period after inoculation with dilutions within the dynamic range of ELISA absorbance - (Peer Reviewed Journal)
Combinatorial treatment of brain samples from sheep with scrapie using sodium percarbonate, sodium dodecyl sulfate, and proteinase K increases survival time in inoculated susceptible sheep Reprint Icon - (Peer Reviewed Journal)
Harm, T.A., Smith, J.D., Cassmann, E.D., Greenlee, J.J. 2022. Combinatorial treatment of brain samples from sheep with scrapie using sodium percarbonate, sodium dodecyl sulfate, and proteinase K increases survival time in inoculated susceptible sheep. Research in Veterinary Science. 152:497-503. https://doi.org/10.1016/j.rvsc.2022.09.002.
Comparison of protein hallmarks of ferroptosis in mice with prion disease - (Abstract Only)
Transmission of raccoon passaged chronic wasting disease agent to white-tailed deer Reprint Icon - (Peer Reviewed Journal)
Cassmann, E.D., Frese, A.J., Moore, S.J., Greenlee, J.J. 2022. Transmission of raccoon passaged chronic wasting disease agent to white-tailed deer. Viruses. 14(7). Article 1578. https://doi.org/10.3390/v14071578.
Exploring the possibility of CWD transmission through artificial insemination of semen from CWD positive bucks - (Trade Journal)
Cassmann, E.D., Greenlee, J.J. 2022. Exploring the possibility of CWD transmission through artificial insemination of semen from CWD positive bucks. North American Deer Farmer. p. 107-109.
Cattle with the EK211 PRNP polymorphism are susceptible to the H-type bovine spongiform encephalopathy agent from either E211K or wild type donors after oronasal inoculation Reprint Icon - (Abstract Only)
Greenlee, J.J., Cassmann, E.D., Moore, S., West Greenlee, H.M. 2022. Cattle with the EK211 PRNP polymorphism are susceptible to the H-type bovine spongiform encephalopathy agent from either E211K or wild type donors after oronasal inoculation. Prion 2022 Conference abstracts: pushing the boundaries. 16(1):150. https://doi.org/10.1080/19336896.2022.2091286.
The chronic wasting disease agent from white-tailed deer is highly infectious to humanized mice after passage through raccoons Reprint Icon - (Abstract Only)
Cassmann, E.D., Qi, X., Kong, Q., Greenlee, J.J. 2022. The chronic wasting disease agent from white-tailed deer is highly infectious to humanized mice after passage through raccoons. Prion 2022 Conference abstracts: pushing the boundaries. 16(1):120. https://doi.org/10.1080/19336896.2022.2091286.
The chronic wasting disease agent from white-tailed deer fails to adapt to sheep upon second passage Reprint Icon - (Abstract Only)
Frese, A.J., Cassmann, E.D., West Greenlee, H.M., Greenlee, J.J. 2022. The chronic wasting disease agent from white-tailed deer fails to adapt to sheep upon second passage. Prion 2022 Conference abstracts: pushing the boundaries. 16(1):144. https://doi.org/10.1080/19336896.2022.2091286.
Application of PMCA to understand CWD prion strains, species barrier and zoonotic potential Reprint Icon - (Abstract Only)
Pritzkow, S., Gorski, D., Ramirez, F., Wang, F., Telling, G.C., Greenlee, J.J., Benestad, S.L., Soto, C. 2022. Application of PMCA to understand CWD prion strains, species barrier and zoonotic potential. Prion 2022 Conference abstracts: pushing the boundaries. 16(1):209. https://doi.org/10.1080/19336896.2022.2091286.
ATYPRION project: assessing the zoonotic potential of interspecies transmission of CWD isolates to livestock (preliminary results) Reprint Icon - (Abstract Only)
Vidal, E., Espinosa, J., Giler, S., Ordonez, M., Cantero, G., Beringue, V., Greenlee, J.J., Torres, J. 2022. ATYPRION project: assessing the zoonotic potential of interspecies transmission of CWD isolates to livestock (preliminary results). Prion 2022 Conference abstracts: pushing the boundaries. 16(1):240. https://doi.org/10.1080/19336896.2022.2091286.
Generation of human chronic wasting disease in transgenic mice Reprint Icon - (Abstract Only)
Wang, Z., Qin, K., Camacho, M.V., Cali, I., Yuan, J., Shen, P., Gilliland, T., Shah, S., Gerasimenko, M., Tang, M., Rajamanickam, S., Yadati, A., Schonberger, L.B., Greenlee, J.J., Kong, Q., Mastrianni, J.A., Zou, W. 2022. Generation of human chronic wasting disease in transgenic mice. Prion 2022 Conference abstracts: pushing the boundaries. 16(1):243. https://doi.org/10.1080/19336896.2022.2091286.
Second passage of scrapie in white-tailed deer is discernable from chronic wasting disease Reprint Icon - (Abstract Only)
Lambert, Z., West Greenlee, H.M., Cassmann, E.D., Greenlee, J.J. 2022. Second passage of scrapie in white-tailed deer is discernable from chronic wasting disease. Prion 2022 Conference abstracts: pushing the boundaries. 16(1):181. https://doi.org/10.1080/19336896.2022.2091286.
Generation of human chronic wasting disease in transgenic mice - (Proceedings)
Wang, Z., Qin, K., Camacho, M.V., Cali, I., Yuan, J., Shen, P., Greenlee, J.J., Kong, Q., Mastrianni, J.A., Zou, W. 2022. Generation of human chronic wasting disease in transgenic mice. Proceedings of 10th Iberian Congress on Prions, May 19-20, 2022, Vila Real, Portugal.
Chronic wasting disease isolates from Norway and North America lack evidence of common strain characteristics - (Trade Journal)
Cassmann, E.D., Greenlee, J.J. 2022. Chronic wasting disease isolates from Norway and North America lack evidence of common strain characteristics. North American Deer Farmer. p. 112.
Increased attack rates and decreased incubation periods in raccoons with chronic wasting disease passaged through meadow voles Reprint Icon - (Peer Reviewed Journal)
Moore, S., Carlson, C., Schneider, J., Johnson, C., Greenlee, J.J. 2022. Increased attack rates and decreased incubation periods in raccoons with chronic wasting disease passaged through meadow voles. Emerging Infectious Diseases. 28(4). Article 210271. https://doi.org/10.3201/eid2804.210271.
Differential accumulation of misfolded prion strains in natural hosts of prion diseases Reprint Icon - (Review Article)
Lambert, Z., Greenlee, J.J., Cassmann, E.D., West Greenlee, H.M. 2021. Differential accumulation of misfolded prion strains in natural hosts of prion diseases. Viruses. 13(12). Article 2453. https://doi.org/10.3390/v13122453.
Why are specific tissue samples required for detection of chronic wasting disease prions - (Trade Journal)
Harm, T., Cassmann, E.D., Greenlee, J.J. 2021. Why are specific tissue samples required for detection of chronic wasting disease prions. North American Deer Farmer. p. 40-43.
Attempted transmission of the CWD agent to deer with potentially resistant prion protein genotypes - (Trade Journal)
Greenlee, J.J., Cassmann, E.D. 2022. Attempted transmission of the CWD agent to deer with potentially resistant prion protein genotypes. North American Deer Farmer. p. 117.
Generation of human chronic wasting disease in transgenic mice Reprint Icon - (Peer Reviewed Journal)
Wang, Z., Qin, K., Camacho, M., Ignazio, C., Yuan, J., Shen, P., Greenlee, J.J., Kong, Q., Mastrianni, J., Zou, W. 2021. Generation of human chronic wasting disease in transgenic mice. Acta Neuropathologica. 9. Article 158. https://doi.org/10.1186/s40478-021-01262-y.
Experimental oronasal transmission of chronic wasting disease agent from white-tailed deer to Suffolk sheep Reprint Icon - (Peer Reviewed Journal)
Cassmann, E.D., Moore, S., Greenlee, J.J. 2021. Experimental oronasal transmission of chronic wasting disease agent from white-tailed deer to Suffolk sheep. Emerging Infectious Diseases. 27(12). https://doi.org/10.3201/eid2712.204978.
Decrease in skin prion-seeding activity of prion-infected mice treated with a compound against human and animal prions: a first possible biomarker for prion therapeutics Reprint Icon - (Peer Reviewed Journal)
Ding, M., Teruya, K., Zhang, W., Lee, H., Yuan, J., Oguma, A., Camacho, M., Mitchell, M., Greenlee, J.J., Zou, W., Kong, Q., Doh-Ura, K., Cui, L. 2021. Decrease in skin prion-seeding activity of prion-infected mice treated with a compound against human and animal prions: a first possible biomarker for prion therapeutics. Molecular Neurobiology. https://doi.org/10.1007/s12035-021-02418-6.
RT-QuIC detection of PrPsc in fecal samples from CWD infected White-tailed Deer using Bank Vole Substrate Reprint Icon - (Peer Reviewed Journal)
Hwang, S., Greenlee, J.J., Nicholson, E.M. 2021. RT-QuIC detection of PrPsc in fecal samples from CWD infected White-tailed Deer using Bank Vole Substrate. Frontiers in Veterinary Science. 8. Article 643754. https://doi.org/10.3389/fvets.2021.643754.
Transmission of the atypical/nor98 scrapie agent to suffolk sheep with VRQ/ARQ, ARQ/ARQ, and ARQ/ARR genotypes Reprint Icon - (Peer Reviewed Journal)
Cassmann, E.D., Mammadova, J., Benestad, S., Moore, S., Greenlee, J.J. 2021. Transmission of the atypical/nor98 scrapie agent to suffolk sheep with VRQ/ARQ, ARQ/ARQ, and ARQ/ARR genotypes. PLoS ONE. 16(2). Article e0246503. https://doi.org/10.1371/journal.pone.0246503.
Second passage of chronic wasting disease of mule deer in sheep compared to classical scrapie after intracranial inoculation Reprint Icon - (Peer Reviewed Journal)
Cassmann, E.D., Frese, R., Greenlee, J.J. 2021. Second passage of chronic wasting disease of mule deer in sheep compared to classical scrapie after intracranial inoculation. Journal of Veterinary Diagnostic Investigation. 33(4):711-720. https://doi.org/10.1177/10406387211017615.
Autoclave treatment of the classical scrapie agent US No. 13-7 and experimental inoculation to susceptible VRQ/ARQ sheep via the oral route results in decreased transmission efficiency Reprint Icon - (Peer Reviewed Journal)
Cassmann, E.D., Mammadova, N., Greenlee, J.J. 2020. Autoclave treatment of the classical scrapie agent US No. 13-7 and experimental inoculation to susceptible VRQ/ARQ sheep via the oral route results in decreased transmission efficiency. PLoS ONE. 15(12). Article e0243009. https://doi.org/10.1371/journal.pone.0243009.
Successful transmission of the chronic wasting disease (CWD) agent to white-tailed deer by intravenous blood transfusion Reprint Icon - (Peer Reviewed Journal)
Mammadova, N., Cassman, E., Greenlee, J.J. 2020. Successful transmission of the chronic wasting disease (CWD) agent to white-tailed deer by intravenous blood transfusion. Research in Veterinary Science. 133:304-306. https://doi.org/10.1016/j.rvsc.2020.10.009.
Evaluation of antemortem diagnostic techniques in goats naturally infected with scrapie Reprint Icon - (Peer Reviewed Journal)
Mammadova, N., West Greenlee, H.M., Moore, J.S., Hwang, S., Lehmkuhl, A.D., Nicholson, E.M., Greenlee, J.J. 2020. Evaluation of antemortem diagnostic techniques in goats naturally infected with scrapie. Frontiers in Veterinary Science. 7. Article 517862. https://doi.org/10.3389/fvets.2020.517862.
Bovine adapted transmissible mink encephalopathy is similar to L-BSE after passage through sheep with the VRQ/VRQ genotype but not VRQ/ARQ Reprint Icon - (Peer Reviewed Journal)
Cassmann, E.D., Moore, Sara, J, S.J., Kokemullar, R.D., Balkema-Buschman, A., Groschup, M., Nicholson, E.M., Greenlee, J.J. 2020. Bovine adapted transmissible mink encephalopathy is similar to L-BSE after passage through sheep with the VRQ/VRQ genotype but not VRQ/ARQ. BMC Veterinary Research. 16. Article 383. https://doi.org/10.1186/s12917-020-02611-0.
CWD research update from NADC - (Trade Journal)
Cassmann, E.D., Greenlee, J.J. 2020. CWD research update from NADC. North American Deer Farmer. p.72-73.
Experimental inoculation of CD11c+ B1 lymphocytes, CD68+ macrophages, or platelet-rich plasma from scrapie-infected sheep into susceptible sheep results in variable infectivity Reprint Icon - (Peer Reviewed Journal)
Mammadova, N., Cassmann, E., Moore, S., Nicholson, E.M., Greenlee, J.J. 2020. Experimental inoculation of CD11c+ B1 lymphocytes, CD68+ macrophages, or platelet-rich plasma from scrapie-infected sheep into susceptible sheep results in variable infectivity. Access Microbiology. 2(9). https://doi.org/10.1099/acmi.0.000155.
Efficient transmission of US scrapie agent by intralingual route to genetically susceptible sheep with a low dose inoculum Reprint Icon - (Peer Reviewed Journal)
Mammadova, N., Cassmann, E., Greenlee, J.J. 2020. Efficient transmission of US scrapie agent by intralingual route to genetically susceptible sheep with a low dose inoculum. Research in Veterinary Science. 132:217-220. https://doi.org/10.1016/j.rvsc.2020.06.010.
Ex-vivo brain slice culture model for chronic wasting disease Reprint Icon - (Peer Reviewed Journal)
Kondru, N., Manne, S., Kokemuller, R., Greenlee, J.J., West Greenlee, M.H., Nichols, T., Kong, Q., Anantharam, V., Kanthasamy, A., Halbur, P., Kanthasamy, A. 2020. Ex-vivo brain slice culture model for chronic wasting disease. Scientific Reports. 10:7640. https://doi.org/10.1038/s41598-020-64456-9.
An ex vivo brain slice culture model of chronic wasting disease: implications for disease pathogenesis and therapeutic development Reprint Icon - (Peer Reviewed Journal)
Kondru, N., Manne, S., Kokemuller, R., Greenlee, J.J., West Greenlee, M.H., Nichols, T., Kong, Q., Anantharam, V., Kanthasamy, A., Halbur, P., Kanthasamy, A. 2020. An ex vivo brain slice culture model of chronic wasting disease: implications for disease pathogenesis and therapeutic development. Scientific Reports. 10. Article 7640. https://doi.org/10.1038/s41598-020-64456-9.
Experimental study using multiple strains of prion disease in cattle reveals an inverse relationship between incubation time and misfolded prion accumulation, neuroinflammation, and autophagy Reprint Icon - (Peer Reviewed Journal)
Mammadova, N., West Greenlee, M.H., Moore, S.J., Sakaguchi, D.S., Greenlee, J.J. 2020. Experimental study using multiple strains of prion disease in cattle reveals an inverse relationship between incubation time and misfolded prion accumulation, neuroinflammation, and autophagy. American Journal of Pathology. 190(7):1461-1473. https://doi.org/10.1016/j.ajpath.2020.03.006.
Experimental study using multiple strains of prion disease in cattle reveals an inverse relationship between incubation time and misfolded prion accumulation, neuroinflammation and autophagy Reprint Icon - (Peer Reviewed Journal)
Mammadova, N., West Greenlee, M.H., Moore, S.J., Sakaguchi, D.S., Greenlee, J.J. 2020. Experimental study using multiple strains of prion disease in cattle reveals an inverse relationship between incubation time and misfolded prion accumulation, neuroinflammation and autophagy. Scientific Reports. 190(7):1461-1473. https://doi.org/10.1016/j.ajpath.2020.03.006.
Retina as a model to study in vivo transmission of alpha-synuclein in the A53T mouse model of Parkinson’s disease Reprint Icon - (Book / Chapter)
Mammadova, N., Baron, T., Verchere, J., Greenlee, J.J., West Greenlee, M.H. 2021. Retina as a model to study in vivo transmission of alpha-synuclein in the A53T mouse model of Parkinson’s disease. In: Singh S.R., Hoffman R.M., Singh A. (eds) Mouse Genetics. Methods in Molecular Biology. New York, NY: Humana. p.75-85. https://doi.org/10.1007/978-1-0716-1008-4_5.
Novel strain of the chronic wasting disease agent isolated from experimentally inoculated elk with LL132 prion protein Reprint Icon - (Peer Reviewed Journal)
Moore, S.J., Tatum, T.L., Hwang, S., Vrentas, C.E., West Greenlee, M.H., Kong, Q., Nicholson, E.M., Greenlee, J.J. 2020. Novel strain of the chronic wasting disease agent isolated from experimentally inoculated elk with LL132 prion protein. Scientific Reports. 10(3148). https://doi.org/10.1038/s41598-020-59819-1.
Novel strain of the chronic wasting disease agent isolated from experimentally inoculated elk with LL132 prion protein Reprint Icon - (Peer Reviewed Journal)
Moore, S., Tatum, T.L., Hwang, S., Vrentas, C.E., West Greenlee, M.H., Kong, Q., Nicholson, E.M., Greenlee, J.J. 2020. Novel strain of the chronic wasting disease agent isolated from experimentally inoculated elk with LL132 prion protein. Nature Scientific Reports. 10:1-13. Article 3148. https://doi.org/10.1038/s41598-020-59819-1.
Pathogenesis, detection, and control of scrapie in sheep Reprint Icon - (Review Article)
Cassman, E.D., Greenlee, J.J. 2020. Pathogenesis, detection, and control of scrapie in sheep. American Journal of Veterinary Research. 81(7):600-614. https://doi.org/10.2460/ajvr.81.7.600.
Role of donor genotype in RT-QuIC seeding activity of chronic wasting disease prions using human and bank vole substrates Reprint Icon - (Peer Reviewed Journal)
Hwang, S., Greenlee, J.J., Nicholson, E.M. 2020. Role of donor genotype in RT-QuIC seeding activity of chronic wasting disease prions using human and bank vole substrates. Scientific Reports. 15(1):e0227487. https://doi.org/10.1371/journal.pone.0227487.
The pathogenesis, detection, and control of scrapie in sheep Reprint Icon - (Review Article)
Cassmann, E.D., Greenlee, J.J. 2020. The pathogenesis, detection, and control of scrapie in sheep. American Journal of Veterinary Research. 81(7):600-614. https://doi.org/10.2460/ajvr.81.7.600.
Sheep are susceptible to the agent of TME by intracranial inoculation and have evidence of infectivity in lymphoid tissues Reprint Icon - (Peer Reviewed Journal)
Cassmann, E.D., Moore, S.J., Smith, J.D., Greenlee, J.J. 2019. Sheep are susceptible to the agent of TME by intracranial inoculation and have evidence of infectivity in lymphoid tissues. Frontiers in Veterinary Science. 6:430. https://doi.org/10.3389/fvets.2019.00430.
Sheep are susceptible to the bovine adapted transmissible mink encephalopathy agent by intracranial inoculation and have evidence of infectivity in lymphoid tissues Reprint Icon - (Peer Reviewed Journal)
Cassmann, E.D., Moore, S.J., Smith, J.D., Greenlee, J.J. 2019. Sheep are susceptible to the bovine adapted transmissible mink encephalopathy agent by intracranial inoculation and have evidence of infectivity in lymphoid tissues. Frontiers in Veterinary Science. 6. https://doi.org/10.3389/fvets.2019.00430.
The chronic wasting disease agent from mule deer has widespread lymphoid distribution in sheep after second passage intracranial inoculation - (Abstract Only)
Cassmann, E., Frese, R.D., Greenlee, J.J. 2019. The chronic wasting disease agent from mule deer has widespread lymphoid distribution in sheep after second passage intracranial inoculation. 2019 American College of Veterinary Pathologists Annual Meeting. Paper No. EY-56.
In-situ temporospatial characterization of the glial response to prion infection Reprint Icon - (Peer Reviewed Journal)
Michael, A.V., Greenlee, J.J., Harm, T.A., Moore, S.J., Zhang, M., Lind, M.S., West Greenlee, M.H., Smith, J.D. 2019. In-situ temporospatial characterization of the glial response to prion infection. Veterinary Pathology. 57(1):90-107. https://doi.org/10.1177/0300985819861708.
The agent of transmissible mink encephalopathy passaged in sheep is similar to BSE-L Reprint Icon - (Abstract Only)
Cassmann, E.D., Greenlee, J.J., Balkema-Buschmann, A., Groschup, M.H., Moore, S.J., Kokemuller, R. 2019. The agent of transmissible mink encephalopathy passaged in sheep is similar to BSE-L. Prion. 13. Article 49. https://doi.org/10.1080/19336896.2019.1615197.
Incubation periods of classical and atypical bovine spongiform encephalopathies (BSE) reflect an inverse relationship between PrPSc-associated neuroinflammation and autophagy Reprint Icon - (Abstract Only)
Mammadova, N., West Greenlee, M.H., Moore, S.J., Sakaguchi, D.S., Greenlee, J.J. 2019. Incubation periods of classical and atypical bovine spongiform encephalopathies (BSE) reflect an inverse relationship between PrPSc-associated neuroinflammation and autophagy. Prion. 13. Article 242. https://doi.org/10.1080/19336896.2019.1615197.
Scrapie in white-tailed deer: a strain of the CWD agent that efficiently transmits to sheep? Reprint Icon - (Abstract Only)
Greenlee, J.J., Kokemuller, R.D., Moore, S.J., West Greenlee, M.H. 2019. Scrapie in white-tailed deer: a strain of the CWD agent that efficiently transmits to sheep? Prion. 13. Article 223. https://doi.org/10.1080/19336896.2019.1615197.
The diagnosis of the prion diseases of animals - (Abstract Only)
Greenlee, J.J. 2019. The diagnosis of the prion diseases of animals [abstract].2019 Tri-State plus One Histology Symposium. 05/08/2019.
Raccoons accumulate PrPSc after intracranial inoculation with the agents of chronic wasting disease (CWD) or transmissible mink encephalopathy (TME) but not atypical scrapie Reprint Icon - (Peer Reviewed Journal)
Moore, S.J., Smith, J.D., Richt, J., Greenlee, J.J. 2019. Raccoons accumulate PrPSc after intracranial inoculation with the agents of chronic wasting disease (CWD) or transmissible mink encephalopathy (TME) but not atypical scrapie. Journal of Veterinary Diagnostic Investigation. 31(2):200-209. https://doi.org/10.1177/1040638718825290.
Scrapie agent from different genotypes of sheep show different seeding activity with elk recombinant prion proteins in RT-QuIC Reprint Icon - (Peer Reviewed Journal)
Hwang, S., Greenlee, J.J., Vance, N.M., Nicholson, E.M. 2018. Source genotype influence on cross species transmission of transmissible spongiform encephalopathies evaluated by RT-QuIC. PLoS One. 13(12):e0209106. https://doi.org/10.1371/journal.pone.0209106.
Sheep with the homozygous lysine-171 prion protein genotype are resistant to classical scrapie after experimental oronasal inoculation Reprint Icon - (Peer Reviewed Journal)
Cassmann, E.D., Moore, S.J., Smith, J.D., Greenlee, J.J. 2018. Sheep with the homozygous lysine-171 prion protein genotype are resistant to classical scrapie after experimental oronasal inoculation. Veterinary Pathology. 56(3):409-417. https://doi.org/10.1177/0300985818817066.
Accelerated accumulation of retinal alpha-synuclein (pSer129) and tau, neuroinflammation, and autophagic dysregulation in a seeded mouse model of Parkinson's disease Reprint Icon - (Peer Reviewed Journal)
Mammadova, N., Summers, C.M., Kokemuller, R.D., He, Q., Ding, S., Baron, T., Yu, C., Valentine, R.J., Sakaguchi, D.S., Kanthasamy, A.G., Greenlee, J.J., West Greenlee, M.H. 2019. Accelerated accumulation of retinal alpha-synuclein (pSer129) and tau, neuroinflammation, and autophagic dysregulation in a seeded mouse model of Parkinson's disease. Neurobiology of Disease. 121:1-16. https://doi.org/10.1016/j.nbd.2018.09.013.
Sheep are susceptible to the agent of transmissible mink encephalopathy after intracranial inoculation - (Abstract Only)
Distribution of atypical porcine pestivirus in the cerebellum of newborn piglets following in utero inoculation - (Abstract Only)
Review: update on classical and atypical scrapie in sheep and goats Reprint Icon - (Review Article)
Greenlee, J.J. 2018. Review: update on classical and atypical scrapie in sheep and goats. Veterinary Pathology. 56(1):6-16. https://doi.org/10.1177/0300985818794247.
The agent of H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism transmits after oronasal challenge - (Abstract Only)
Greenlee, J.J., Moore, S.J., West Greenlee, M.H. 2018. The agent of H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism transmits after oronasal challenge. Prion 2018, May 22-25, 2018, Santiago de Compostela, Spain. Paper No. P98, page 116.
Retinopathy in a mouse model of Parkinson’s disease: seeding of synucleinopathy induces accumulation of phosphorylated alpha-synuclein (pSer129) and tau, neuroinflammation, metabolic dysregulation, and cell death - (Abstract Only)
Mammadova, N., Kokemuller, R., Summers, C.M., He, Q., Ding, S., Baron, T., Yu, C., Valentine, R.J., Sakaguchi, D.S., Kanthasamy, A.G., Greenlee, J.J., West Greenlee, M.H. 2018. Retinopathy in a mouse model of Parkinson's disease: seeding of synucleinopathy induces accumulation of phosphorylated alpha-synuclein (pSer129) and tau, neuroinflammation, metabolic dysregulation, and cell death. Prion 2018, May 22-25, 2018, Santiago de Compostela, Spain. Paper No. P144. p. 149-150.
The agent of chronic wasting disease from pigs is infectious in transgenic mice expressing human PRNP - (Abstract Only)
Moore, S.J., Kokemuller, R.D., West-Greenlee, M.H., Balkema-Buschmann, A., Groschup, M.H., Greenlee, J.J. 2018. The agent of chronic wasting disease from pigs is infectious in transgenic mice expressing human PRNP. Prion 2018, Santiago de Compostela, Spain, May 22-25, 2018. Paper No. WA15, page 44.
RT-QuIC seeding activity using elk recombinant prion proteins discriminates scrapie isolates - (Abstract Only)
Hwang, S., Greenlee, J.J., Vance, N.M., Nicholson, E.M. 2018. RT-QuIC seeding activity using elk recombinant prion proteins discriminates scrapie isolates. Prion 2018. Poster No. P58, p. 85.
Pathologic and biochemical characterization of PrPSc from elk with PRNP polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent Reprint Icon - (Peer Reviewed Journal)
Moore, S.J., Vrentas, C.E., Hwang, S., West Greenlee, M.H., Nicholson, E.M., Greenlee, J.J. 2018. Pathologic and biochemical characterization of PrPSc from elk with PRNP polymorphisms at codon 132 after experimental infection with the chronic wasting disease agent. BMC Veterinary Research. 14(1):80. https://doi.org/10.1186/s12917-018-1400-9.
Detection of BSE prions by RT-QuIC in cattle with subclinical BSE Reprint Icon - (Peer Reviewed Journal)
Hwang, S., West Greenlee, M.H., Balkema-Buschmann, A., Groschup, M.H., Nicholson, E.M., Greenlee, J.J. 2018. Real-time quaking-induced conversion detection of bovine spongiform encephalopathy prions in a subclinical steer. Frontiers in Veterinary Science. 4:242. https://doi.org/10.3389/fvets.2017.00242.
Effects of a naturally occurring amino acid substitution in bovine PrP: a model for inherited prion disease in a natural host species - (Peer Reviewed Journal)
Vrentas, C.E., Greenlee, J.J., Foster, G.H., West, J., Jahnke, M.M., Schmidt, M.T., Nicholson, E.M. 2017. Effects of a naturally occurring amino acid substitution in bovine PrP: a model for inherited prion disease in a natural host species. BMC Research Notes. 10:759. https://doi.org/10.1186/s13104-017-3085-8.
Experimental transmission of the chronic wasting disease agent to swine after oral or intracranial inoculation - (Peer Reviewed Journal)
Moore, S.J., West Greenlee, M.H., Kondru, N., Manne, S., Smith, J.D., Kunkle, R.A., Kanthasamy, A., Greenlee, J.J. 2017. Experimental transmission of the chronic wasting disease agent to swine after oral or intracranial inoculation. Journal of Virology. 91(19):e00926-17. https://doi.org/10.1128/JVI.00926-17.
Pathology in practice: pituitary adenoma in a sheep Reprint Icon - (Peer Reviewed Journal)
Smith, J.D., Harm, T.A., Greenlee, J.J. 2019. Pathology in practice: pituitary adenoma in a sheep. Journal of the American Veterinary Medical Association. 254(7):819-821. https://doi.org/10.2460/javma.254.7.819.
Disease-associated prion protein detected in lymphoid tissues from pigs challenged with the agent of chronic wasting disease - (Abstract Only)
Accumulation of phosphorylated alpha-synuclein (p129S) and retinal pathology in a mouse model of Parkinson's disease - (Abstract Only)
Passage of scrapie to deer results in a new phenotype upon return passage to sheep - (Abstract Only)
Lasting retinal injury in a mouse model of blast-induced trauma - (Peer Reviewed Journal)
Mammadova, N., Ghaisas, S., Zenitsky, G., Sakaguchi, D.S., Kanthasamy, A.G., Greenlee, J.J., West Greenlee, M.H. 2017. Lasting retinal injury in a mouse model of blast-induced trauma. American Journal of Pathology. 187(7):1459-1472. doi:10.1016/j.ajpath.2017.03.005.
Use of bovine recombinant prion protein and real-time quaking-induced conversion to detect transmissible mink encephalopathy prions and discriminate classical and atypical L- and H-type bovine spongiform encephalopathy - (Peer Reviewed Journal)
Hwang, S., Greenlee, J.J., Nicholson, E.M. 2017. Use of bovine recombinant prion protein and real-time quaking-induced conversion to detect cattle transmissible mink encephalopathy prions and discriminate classical and atypical L- and H-type bovine spongiform encephalopathy. PLoS One. 12(2):e0172391.
Integrated organotypic slice cultures and RT-QuIC (OSCAR) assay: implications for translational discovery in protein misfolding diseases - (Peer Reviewed Journal)
Kondru, N., Manne, S., Greenlee, J., West Greenlee, H., Anantharam, V., Halbur, P., Kanthasamy, A., Kanthasamy, A. 2017. Integrated organotypic slice cultures and RT-QuIC (OSCAR) assay: implications for translational discovery in protein misfolding diseases. Scientific Reports. 7:43155. doi:10.1038/srep43155.
Survival time and stability properties of disease-associated prion protein in chronic wasting disease of elk - (Abstract Only)
Temporospatial distribution of microglial activation in a murine model of scrapie - (Abstract Only)
Transmission of chronic wasting disease of white-tailed deer to Suffolk sheep following intracranial inoculation - (Abstract Only)
Scrapie in swine: a diagnostic challenge - (Peer Reviewed Journal)
Greenlee, J.J., Kunkle, R.A., Smith, J.D., West Greenlee, M.H. 2016. Scrapie in swine: a diagnostic challenge. Food Safety. 4(4):110-114.
A comparison of classical and H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism in wild type and EK211 cattle following intracranial inoculation - (Peer Reviewed Journal)
Moore, S.J., West Greenlee, M.H., Smith, J.D., Vrentas, C.E., Nicholson, E.M., Greenlee, J.J. 2016. A comparison of classical and H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism in wild type and EK211 cattle following intracranial inoculation. Frontiers in Veterinary Science. 3:78.
Horizontal transmission of chronic wasting disease in reindeer - (Peer Reviewed Journal)
Moore, S., Kunkle, R., Greenlee, M., Nicholson, E., Richt, J., Hamir, A., Waters, W., Greenlee, J. 2016. Horizontal transmission of chronic wasting disease in reindeer. Emerging Infectious Diseases. 22(12):2142-2145. doi:10.3201/eid2212.160635.
Genetic variation of the prion protein gene (PRNP) in alpaca (Vicugna pacos) - (Peer Reviewed Journal)
Vermette, M.S., Schleining, J.A., Greenlee, J.J., Smith, J.D. 2016. Genetic variation of the prion protein gene (PRNP) in alpaca (Vicugna pacos). Gene Reports. 4:213–217.
Temporal resolution of misfolded prion protein transport, accumulation, glial activation, and neuronal death in the retinas of mice inoculated with scrapie - (Peer Reviewed Journal)
West Greenlee, M.H., Lind, M., Kokemuller, R., Mammadova, N., Kondru, N., Manne, S., Smith, J., Kanthasamy, A., Greenlee, J. 2016. Temporal resolution of misfolded prion protein transport, accumulation, glial activation, and neuronal death in the retinas of mice inoculated with scrapie. American Journal of Pathology. 186(9):2302–2309.
Temporal resolution of PrPSc transport, PrPSc accumulation, activation of glia and neuronal death in retinas from C57Bl/6 mice inoculated with RML scrapie: Relevance to biomarkers of prion disease progression - (Abstract Only)
Prion infectivity detected in swine challenged with chronic wasting disease via the intracerebral or oral route - (Abstract Only)
Scrapie in swine: a diagnostic challenge - (Abstract Only)
Limited amplification of chronic wasting disease prions in the peripheral tissues of intracerebrally inoculated cattle - (Peer Reviewed Journal)
Haley, N.J., Siepker, C., Greenlee, J.J., Richt, J.A. 2016. Limited amplification of chronic wasting disease prions in the peripheral tissues of intracerebrally inoculated cattle. Journal of General Virology. 97:1720-1724.
Oral inoculation of neonatal Suffolk sheep with the agent of classical scrapie results in PrPSc accumulation in sheep with the PRNP ARQ/ARQ but not the ARQ/ARR genotype - (Peer Reviewed Journal)
Greenlee, J.J., Smith, J.D., Hamir, A.N. 2016. Oral inoculation of neonatal Suffolk sheep with the agent of classical scrapie results in PrPSc accumulation in sheep with the PRNP ARQ/ARQ but not the ARQ/ARR genotype. Research in Veterinary Science. 105:188–191.
Comparison of two US sheep scrapie isolates supports identification as separate strains - (Peer Reviewed Journal)
Moore, S.J., Smith, J.D., West Greenlee, M.H., Nicholson, E.M., Richt, J.A., Greenlee, J.J. 2016. Comparison of two US sheep scrapie isolates supports identification as separate strains. Veterinary Pathology. 53(6):1187-1196. doi: 10.1177/0300985816629712.
Antemortem detection of chronic wasting disease prions in nasal brush collections and rectal biopsies from white-tailed deer by real time quaking-induced conversion - (Peer Reviewed Journal)
Haley, N.J., Siepker, C., Walter, W.D., Thomsen, B.V., Greenlee, J.J., Lehmkuhl, A.D., Richt, J.A. 2016. Antemortem detection of chronic wasting disease prions in nasal brush collections and rectal biopsy specimens from white-tailed deer by real time quaking-induced conversion. Journal of Clinical Microbiology. 54(4):1108-1116.
Scrapie transmits to white-tailed deer by the oral route and has a molecular profile similar to chronic wasting disease - (Abstract Only)
Transmission of chronic wasting disease to sentinel reindeer (Rangifer tarandus tarandus) - (Abstract Only)
Transmission of scrapie prions to primate after an extended silent incubation period - (Peer Reviewed Journal)
Comoy, E.E., Mikol, J., Luccantoni-Freire, S., Correia, E., Lescoutra-Etchegaray, N., Durand, V., Dehen, C., Andreoletti, O., Casalone, C., Richt, J.A., Greenlee, J.J., Baron, T., Benestad, S., Brown, P., Deslys, J. 2015. Transmission of scrapie prions to primate after an extended silent incubation period. Scientific Reports. 5:11573.
H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism: clinical and pathologic features in wild-type and E211K cattle following intracranial inoculation - (Abstract Only)
Moore, S.J., West Greenlee, M.H., Smith, J., Nicholson, E., Vrentas, C., Greenlee, J. 2015. H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism: clinical and pathologic features in wild-type and E211K cattle following intracranial inoculation. Prion 2015. p. S5.
Transmission of the agent of sheep scrapie to deer results in PrPSc with two distinct molecular profiles - (Abstract Only)
Greenlee, J., Moore, S.J., Smith, J.., West Greenlee, M.H., Kunkle, R. 2015. Scrapie transmits to white-tailed deer by the oral route and has a molecular profile similar to chronic wasting disease and distinct from the scrapie inoculum. Prion 2015. p. S62.
Development and characterization of an ex-vivo brain slice culture model of chronic wasting disease - (Abstract Only)
Kondru, N., Greenlee, J., Greenlee, H., Manne, S., Kong, Q., Halbur, P., Kanthasamy, A., Kanthasamy, A. 2015. Development and characterization of an ex-vivo brain slice culture model of chronic wasting disease. Prion 2015. p. S68.
Relationship of PrPSc molecular properties with incubation time in a natural prion disease host: a characterization of three isolates of U.S. sheep scrapie - (Abstract Only)
Vrentas, C., Smith, J., Greenlee, J., Nicholson, E. 2015. Relationship of PrPSc molecular properties with incubation time in a natural prion disease host: a characterization of 3 isolates of US sheep scrapie. Prion 2015. p. S92.
Clinical stage of infection is critical in the antemortem diagnosis of chronic wasting disease in deer and elk - (Abstract Only)
Prion peripheralization is a host-driven trait of prion infection, independent of strain - (Abstract Only)
The transmissible spongiform encephalopathies of livestock - (Review Article)
Greenlee, J.J., West Greenlee, M.,H. 2015. The transmissible spongiform encephalopathies of livestock. ILAR Journal. 56(1):7-25.
Raman spectroscopy reveals spectroscopic changes in histologically normal retinas in a mouse model of alpha-synucleinopathy - (Abstract Only)
hTERT-immortalized ovine microglia propagate natural scrapie isolates - (Peer Reviewed Journal)
Munoz-Gutierrez, J.F., Schneider, D.A., Baszler, T.V., Dinkel, K.D., Greenlee, J.J., Nicholson, E.M., Stanton, J.J. 2015. hTERT-immortalized ovine microglia propagate natural scrapie isolates. Virus Research. 198:35-43.
Lack of prion accumulation in lymphoid tissues of PRNP ARQ/ARR sheep intracranially inoculated with the agent of scrapie - (Peer Reviewed Journal)
Greenlee, J.J., Kunkle, R.A., Richt, J.A., Nicholson, E.M., Hamir, A.N. 2014. Lack of prion accumulation in lymphoid tissues of PRNP ARQ/ARR sheep intracranially inoculated with the agent of scrapie. PLoS One. 9(9):e108029.
Bovine Spongiform Encephalopathy: Atypical Pros and Cons - (Proceedings)
Kehrli Jr, M.E., Greenlee, J.J., Nicholson, E.M. 2014. Bovine Spongiform Encephalopathy: Atypical Pros and Cons. Proceedings of the California Animal Nutrition Conference, May 13-16, 2014, Fresno, California. p. 70-81.
The prion diseases of animals - (Book / Chapter)
Greenlee J.J. 2014. The prion diseases of animals. In: McManus, L.M., Mitchell, R.N., editors. Pathobiology of Human Disease. San Diego: Elsevier. p. 1124-1133.
Time-course study of retinal pathology in C57BL/6 mice infected with RML scrapie - (Abstract Only)
Lind, M., Smith, J.D., Briggie, L., West Greenlee, M., Greenlee, J.J. 2013. Time-course study of retinal pathology in C57BL/6 mice infected with RML scrapie. American College of Veterinary Pathologists Meeting. Available: http://www.acvp.org/index.php/en/2014-11-07-21-58-13/2013-annual-meeting.
Evaluation of the zoonotic potential of transmissible mink encephalopathy - (Peer Reviewed Journal)
Comoy, E.E., Mikol, J., Ruchoux, M., Durand, V., Luccantoni-Freire, S., Dehen, C., Correia, E., Casalone, C., Richt, J.A., Greenlee, J.J., Torres, J.M., Brown, P., Deslys, J. 2013. Evaluation of the zoonotic potential of transmissible mink encephalopathy. Pathogens. 2:(3)520-532.
Characterization of the first case of naturally occurring chronic wasting disease in a captive red deer (Cervus elaphus) in North America - (Abstract Only)
Lehmkuhl, A.L., Thomsen, B.V., Greenlee, J.J., Balachandran, A., O'Rourke, K., Hall, S. 2013. Characterization of the first case of naturally occurring chronic wasting disease in a captive red deer (Cervus elaphus) in North America. Prion 2013. AD-12-Prion (2013).7:22.
Atypical BSE: role of the E211K prion polymorphism - (Abstract Only)
Greenlee, J.J. 2013. Atypical BSE: role of the E211K prion polymorphism. 2013 American Veterinary Medical Association (AVMA) Annual Convention, July 19-23, 2013, Chicago, Illinois. Paper No. 13920. p. 45.