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United States Department of Agriculture

Agricultural Research Service

Related Topics

Justin Greenlee
Virus and Prion Research
Veterinary Medical Science

Phone: (515) 337-7191
Fax: (515) 337-7149

P.O. BOX 70
1920 DAYTON AVE
AMES , IA 50010

Projects
TRANSMISSION, DIFFERENTIATION, AND PATHOBIOLOGY OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES
In-House Appropriated (D)
  Accession Number: 421870

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
A comparison of classical and H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism in wild type and EK211 cattle following intracranial inoculation -
Moore, S.J., West Greenlee, M.H., Smith, J.D., Vrentas, C.E., Nicholson, E.M., Greenlee, J.J. 2016. A comparison of classical and H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism in wild type and EK211 cattle following intracranial inoculation. Frontiers in Veterinary Science. 3:78.
Pathological features of chronic wasting disease in reindeer and demonstration of horizontal transmission -
Genetic variation of the prion protein gene (PRNP) in alpaca (Vicugna pacos) -
Vermette, M.S., Schleining, J.A., Greenlee, J.J., Smith, J.D. 2016. Genetic variation of the prion protein gene (PRNP) in alpaca (Vicugna pacos). Gene Reports. 4:213–217.
Temporal resolution of misfolded prion protein transport, accumulation, glial activation, and neuronal death in the retinas of mice inoculated with scrapie -
West Greenlee, M.H., Lind, M., Kokemuller, R., Mammadova, N., Kondru, N., Manne, S., Smith, J., Kanthasamy, A., Greenlee, J. 2016. Temporal resolution of misfolded prion protein transport, accumulation, glial activation, and neuronal death in the retinas of mice inoculated with scrapie. American Journal of Pathology. 186(9):2302–2309.
Scrapie in swine: a diagnostic challenge -
Prion infectivity detected in swine challenged with chronic wasting disease via the intracerebral or oral route -
Temporal resolution of PrPSc transport, PrPSc accumulation, activation of glia and neuronal death in retinas from C57Bl/6 mice inoculated with RML scrapie: Relevance to biomarkers of prion disease progression -
Limited amplification of chronic wasting disease prions in the peripheral tissues of intracerebrally inoculated cattle -
Haley, N.J., Siepker, C., Greenlee, J.J., Richt, J.A. 2016. Limited amplification of chronic wasting disease prions in the peripheral tissues of intracerebrally inoculated cattle. Journal of General Virology. 97:1720-1724.
Oral inoculation of neonatal Suffolk sheep with the agent of classical scrapie results in PrPSc accumulation in sheep with the PRNP ARQ/ARQ but not the ARQ/ARR genotype -
Greenlee, J.J., Smith, J.D., Hamir, A.N. 2016. Oral inoculation of neonatal Suffolk sheep with the agent of classical scrapie results in PrPSc accumulation in sheep with the PRNP ARQ/ARQ but not the ARQ/ARR genotype. Research in Veterinary Science. 105:188–191.
Comparison of two US sheep scrapie isolates supports identification as separate strains -
Moore, S.J., Smith, J.D., Greenlee, M.H., Nicholson, E.M., Richt, J.A., Greenlee, J.J. 2016. Comparison of two US sheep scrapie isolates supports identification as separate strains. Veterinary Pathology. doi: 10.1177/0300985816629712.
Antemortem detection of chronic wasting disease prions in nasal brush collections and rectal biopsies from white-tailed deer by real time quaking-induced conversion -
Haley, N.J., Siepker, C., Walter, W.D., Thomsen, B.V., Greenlee, J.J., Lehmkuhl, A.D., Richt, J.A. 2016. Antemortem detection of chronic wasting disease prions in nasal brush collections and rectal biopsy specimens from white-tailed deer by real time quaking-induced conversion. Journal of Clinical Microbiology. 54(4):1108-1116.
Scrapie transmits to white-tailed deer by the oral route and has a molecular profile similar to chronic wasting disease -
Transmission of chronic wasting disease to sentinel reindeer (Rangifer tarandus tarandus) -
Transmission of scrapie prions to primate after an extended silent incubation period -
Comoy, E.E., Mikol, J., Luccantoni-Freire, S., Correia, E., Lescoutra-Etchegaray, N., Durand, V., Dehen, C., Andreoletti, O., Casalone, C., Richt, J.A., Greenlee, J.J., Baron, T., Benestad, S., Brown, P., Deslys, J. 2015. Transmission of scrapie prions to primate after an extended silent incubation period. Scientific Reports. 5:11573.
Transmission of the agent of sheep scrapie to deer results in PrPSc with two distinct molecular profiles -
Greenlee, J., Moore, S.J., Smith, J.., West Greenlee, M.H., Kunkle, R. 2015. Scrapie transmits to white-tailed deer by the oral route and has a molecular profile similar to chronic wasting disease and distinct from the scrapie inoculum. Prion 2015. p. S62.
Development and characterization of an ex-vivo brain slice culture model of chronic wasting disease -
Kondru, N., Greenlee, J., Greenlee, H., Manne, S., Kong, Q., Halbur, P., Kanthasamy, A., Kanthasamy, A. 2015. Development and characterization of an ex-vivo brain slice culture model of chronic wasting disease. Prion 2015. p. S68.
Relationship of PrPSc molecular properties with incubation time in a natural prion disease host: a characterization of three isolates of U.S. sheep scrapie -
Vrentas, C., Smith, J., Greenlee, J., Nicholson, E. 2015. Relationship of PrPSc molecular properties with incubation time in a natural prion disease host: a characterization of 3 isolates of US sheep scrapie. Prion 2015. p. S92.
H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism: clinical and pathologic features in wild-type and E211K cattle following intracranial inoculation -
Moore, S.J., West Greenlee, M.H., Smith, J., Nicholson, E., Vrentas, C., Greenlee, J. 2015. H-type bovine spongiform encephalopathy associated with E211K prion protein polymorphism: clinical and pathologic features in wild-type and E211K cattle following intracranial inoculation. Prion 2015. p. S5.
Prion peripheralization is a host-driven trait of prion infection, independent of strain -
Clinical stage of infection is critical in the antemortem diagnosis of chronic wasting disease in deer and elk -
The transmissible spongiform encephalopathies of livestock -
Greenlee, J.J., West Greenlee, M.,H. 2015. The transmissible spongiform encephalopathies of livestock. ILAR Journal. 56(1):7-25.
Raman spectroscopy reveals spectroscopic changes in histologically normal retinas in a mouse model of alpha-synucleinopathy -
hTERT-immortalized ovine microglia propagate natural scrapie isolates -
Munoz-Gutierrez, J.F., Schneider, D.A., Baszler, T.V., Dinkel, K.D., Greenlee, J.J., Nicholson, E.M., Stanton, J.J. 2015. hTERT-immortalized ovine microglia propagate natural scrapie isolates. Virus Research. 198:35-43.
Lack of prion accumulation in lymphoid tissues of PRNP ARQ/ARR sheep intracranially inoculated with the agent of scrapie -
Greenlee, J.J., Kunkle, R.A., Richt, J.A., Nicholson, E.M., Hamir, A.N. 2014. Lack of prion accumulation in lymphoid tissues of PRNP ARQ/ARR sheep intracranially inoculated with the agent of scrapie. PLoS One. 9(9):e108029.
Bovine Spongiform Encephalopathy: Atypical Pros and Cons -
Kehrli Jr, M.E., Greenlee, J.J., Nicholson, E.M. 2014. Bovine Spongiform Encephalopathy: Atypical Pros and Cons. Proceedings of the California Animal Nutrition Conference, May 13-16, 2014, Fresno, California. p. 70-81.
The prion diseases of animals -
Greenlee J.J. 2014. The prion diseases of animals. In: McManus, L.M., Mitchell, R.N., editors. Pathobiology of Human Disease. San Diego: Elsevier. p. 1124-1133.
Time-course study of retinal pathology in C57BL/6 mice infected with RML scrapie -
Lind, M., Smith, J.D., Briggie, L., West Greenlee, M., Greenlee, J.J. 2013. Time-course study of retinal pathology in C57BL/6 mice infected with RML scrapie. American College of Veterinary Pathologists Meeting. Available: http://www.acvp.org/index.php/en/2014-11-07-21-58-13/2013-annual-meeting.
Stability properties of PrPSc from cattle with experimental transmissible spongiform encephalopathies -
Vrentas, C.E., Greenlee, J.J., Baron, T., Caramelli, M., Czub, S., Nicholson, E.M. 2013. Stability properties of PrPSc from cattle with experimental transmissible spongiform encephalopathies: use of a rapid whole homogenate, protease-free assay. BMC Veterinary Research. 9:167.
Evaluation of the zoonotic potential of transmissible mink encephalopathy -
Comoy, E.E., Mikol, J., Ruchoux, M., Durand, V., Luccantoni-Freire, S., Dehen, C., Correia, E., Casalone, C., Richt, J.A., Greenlee, J.J., Torres, J.M., Brown, P., Deslys, J. 2013. Evaluation of the zoonotic potential of transmissible mink encephalopathy. Pathogens. 2:(3)520-532.
Characterization of the first case of naturally occurring chronic wasting disease in a captive red deer (Cervus elaphus) in North America -
Lehmkuhl, A.L., Thomsen, B.V., Greenlee, J.J., Balachandran, A., O'Rourke, K., Hall, S. 2013. Characterization of the first case of naturally occurring chronic wasting disease in a captive red deer (Cervus elaphus) in North America. Prion 2013. AD-12-Prion (2013).7:22.
Atypical BSE: role of the E211K prion polymorphism -
Greenlee, J.J. 2013. Atypical BSE: role of the E211K prion polymorphism. 2013 American Veterinary Medical Association (AVMA) Annual Convention, July 19-23, 2013, Chicago, Illinois. Paper No. 13920. p. 45.
A low molecular-weight ferroxidase is increased in the CSF of sCJD cases: CSF ferroxidase and transferrin as diagnostic biomarkers for sCJD -
Haldar, S., Beveridge, A.J., Wong, J., Singh, A.J., Galimberti, D., Borroni, D., Zhu, X., Blevins, J., Greenlee, J., Perry, G., Mukhopadhyay, C.K., Schmotzer, C., Singh, N. 2014. A low-molecular-weight ferroxidase is increased in the CSF of sCJD Cases: CSF ferroxidase and transferrin as diagnostic biomarkers for sCJD. Antioxidants & Redox Signaling. 19(14):1662-1675.
Detection of PrP(Sc) in peripheral tissues of clinically affected cattle after oral challenge with bovine spongiform encephalopathy -
Franz, M., Eiden, M., Balkema-Buschmann, A., Greenlee, J., Schatzl, H., Fast, C., Richt, J., Hildebrandt, J.P., Groschup, M.H. 2012. Detection of PrP(Sc) in peripheral tissues of clinically affected cattle after oral challenge with bovine spongiform encephalopathy. Journal of General Virology. 93(Pt12):2740-2748.
Relationships between PrPSc stability and incubation time for United States scrapie strains in a natural host system -
Vrentas, C.E., Greenlee, J.J., Tatum, T.L., Nicholson, E.M. 2012. Relationships between PrPSc stability and incubation time for United States scrapie isolates in a natural host system. PLoS ONE. 7(8):e43060.
Comparative aspects of bovine spongiform encephalopathy isolates found in the U.S. -
Greenlee, J.J., Smith, J.D., Vrentas, C.E., West Greenlee, M.H., Nicholson, E.M. 2012. Comparative aspects of bovine spongiform encephalopathy isolates found in the U.S. American College of Veterinary Pathologists 63rd Annual Meeting. Paper No. E-16.
Susceptibility of cattle to the agent of chronic wasting disease from elk after intracranial inoculation -
Greenlee, J.J., Nicholson, E.M., Smith, J.D., Kunkle, R.A., Hamir, A.N. 2012. Susceptibility of cattle to the agent of chronic wasting disease from elk after intracranial inoculation. Journal of Veterinary Diagnostic Investigation. 24(6):1087-1093.
Use of an ELISA-based stability assay to examine host genotype, PrP**Sc stability, and incubation time relationships in U.S. livestock prion strains -
Vrentas, C.E., Nicholson, E.M., Greenlee, J.J. 2012. Use of an ELISA stability assay to examine host genotype, PrPSc stability, and incubation time relationships in U.S. livestock prion strains. 26th Annual Symposium of The Protein Society. p. 211.
Stability of PrP**Sc from cattle inoculated with different BSE strains -
Nicholson, E.M., Vrentas, C.E., Greenlee, J.J. 2012. Stability of PrPSc from cattle inoculated with different BSE strains. 26th Annual Symposium of The Protein Society. p. 210.
Prolonged incubation time in sheep with prion protein containing lysine at position 171 -
Greenlee, J.J., Zhang, Xia, Nicholson, E.M., Kunkle, R.A., Hamir, A.N. 2012. Prolonged incubation time in sheep with prion protein containing lysine at position 171. Journal of Veterinary Diagnostic Investigation. 24(3):554-558.
Experimental Interspecies Transmission Studies of the Transmissible Spongiform Encephalopathies to Cattle: Comparison to Bovine Spongiform Encephalopathy in Cattle -
Hamir, A.N., Kehrli, Jr., M.E., Kunkle, R.A., Greenlee, J.J., Nicholson, E.M., Richt, J.A., Miller, J.M., Cutlip, R.C. 2011. Experimental interspecies transmission studies of the transmissible spongiform encephalopathies to cattle: comparison to bovine spongiform encephalopathy in cattle. Journal of Veterinary Diagnostic Investigation. 23(3):407-420.
PrPSc detection in formalin-fixed paraffin-embedded tissue by ELISA -
Nicholson, E.M., Greenlee, J.J., Hamir, A.N. 2011. PrPSc detection in formalin-fixed paraffin-embedded tissue by ELISA. BMC Research Notes. 4(1):432.
Evaluation of two sets of immunohistochemical and Western blot confirmatory methods in the detection of typical and atypical BSE cases -
Porcario, C., Hall, S.M., Martucci, F., Corona, C., Iulini, B., Perazzini, A.Z., Acutis, P., Hamir, A.N., Loiacono, C.M., Greenlee, J.J., Richt, J.A., Caramelli, M., Casalone, C. 2011. Evaluation of two sets of immunohistochemical and Western blot confirmatory methods in the detection of typical and atypical BSE cases. BMC Research Notes. 4:376.
Salivary prions in sheep and deer -
Tamguney, G., Richt, J.A., Hamir, A.N., Greenlee, J.J., Miller, M.W., Wolfe, L.L., Sirochman, T.M., Young, A.J., Glidden, D.V., Johnson, N.L., Giles, K., Dearmond, S.J., Prusiner, S.B. 2012. Salivary prions in sheep and deer. Prion. 6(1):52-61.
White-Tailed Deer Susceptible to Scrapie by Natural Route of Infection -
Greenlee, J.J., Smith, J.D., Kunkle, R.A. 2010. White-tailed deer susceptible to scrapie by natural route of infection [abstract]. American College of Veterinary Pathologists. Paper No. 81.
Comparison of Transmissible Mink Encephalopathy Isolates in Raccoons -
Smith, J.D., Greenlee, J.J., Hamir, A.N. 2010. Comparison of transmissible mink encephalopathy isolates in raccoons [abstract]. American College of Veterinary Pathologists. Paper No. 145.
G-CSF Analogue Treatment Increases Peripheral Neutrophil Numbers in Pigs - a Potential Alternative for In-Feed Antibiotics -
Loving, C.L., Brockmeier, S.L., Bayles, D.O., Greenlee, J.J., Lager, K.M., Kehrli, Jr., M.E. 2010. G-CSF analogue treatment increases peripheral neutrophil numbers in pigs - a potential alternative for in-feed antibiotics. Conference of Research Workers in Animal Diseases. Paper No. 140.
Bronchial carcinoma in a Red Shouldered Hawk (Buteo lineatus) -
Greenlee, J.J., Nieves, M.A., Myers, R.K. 2011. Bronchial carcinoma in a red-shouldered hawk (Buteo lineatus). Journal of Zoo and Wildlife Medicine. 42(1):153-155.
White-Tailed Deer Susceptible to Scrapie by Natural Route of Infection -
Identification of an Amino Acid Domain Encoded by the Capsid Protein Gene of Porcine Circovirus Type 2 that Modulates Viral Protein Distribution During Replication -
Cheung, A.K., Greenlee, J.J. 2011. Identification of an amino acid domain encoded by the capsid gene of porcine circovirus type 2 that modulates intracellular viral protein distribution during replication. Virus Research. 155(1):358-362.
Clinical features in prion protein-deficient and wild-type cattle inoculated with transmissible mink encephalopathy (TME) -
Vargas, F., Kuroiwa, Y., Hamir, A.N., Greenlee, J.J., Kunkle, R.A., Kangas, T., Kanthasamy, A., Robl, J., Richt, J. A. 2010. Clinical features in prion protein-deficient and wild-type cattle inoculated with transmissible mink encephalopathy (TME) [abstract]. XXVI World Buiatrics Congress 2010, November 14-18, 2010, Santiago, Chile. Available: http://www.kenes.com/buiatrics/cd/pdf/780.pdf.
White-tailed Deer are Susceptible to Sheep Scrapie by Intracerebral Inoculation -
Greenlee, J.J., Smith, J.D., Kunkle, R.A. 2010. White-tailed deer are susceptible to sheep scrapie by intracerebral inoculation [abstract]. Prion 4(3):144.
Experimental Transmission of Chronic Wasting Disease (CWD) from Elk (Cervus elaphus nelsoni) and White-tailed Deer (Odocoileus virginianus) to Fallow Deer (Dama dama) by Intracerebral Route: Final Report -
Hamir, A.N., Greenlee, J.J., Nicholson, E.M., Kunkle, R.A., Richt, J.A., Miller, J.M., Hall, M. 2011. Experimental transmission of chronic wasting disease (CWD) from elk and white-tailed deer to fallow deer by intracerebral route: final report. Canadian Journal of Veterinary Research. 75(2):152-156.
Experimental Inoculation of Spiroplasma mirum and Transmissible Mink Encephalopathy (TME) into Raccoons (Procyon lotor) -
Hamir, A.N., Greenlee, J.J., Stanton, T.B., Smith, J.D., Doucette, S., Kunkle, R.A., Stasko, J.A., Richt, J.A., Kehrli, Jr., M.E. 2011. Experimental inoculation of raccoons (Procyon lotor) with Spiroplasma mirum and transmissible mink encephalopathy (TME). Canadian Journal of Veterinary Research. 75(1):18–24.
Detection of the Abnormal Isoform of the Prion Protein Associated With Chronic Wasting Disease in the Optic Pathways of the Brain and Retina of Rocky Mountain Elk (Cervus elaphus nelsoni) Reprint Icon -
Spraker, T.R., Orourke, K.I., Gidlewski, T.L., Powers, J., Greenlee, J.J., Wild, M.A. 2010. Detection of the Abnormal Isoform of the Prion Protein Associated With Chronic Wasting Disease in the Optic Pathways of the Brain and Retina of Rocky Mountain Elk (Cervus elaphus nelsoni). Veterinary Pathology. 47(3):536-546.
New potentials in TSE diagnostics -
Greenlee, J.J., Smith, J.D., Greenlee, M.S. 2009. New potentials in TSE diagnostics [abstract]. International Veterinary Vaccines and Diagnostics Conference. p. 42.
Lack of Prion Accumulation in Lymphoid Tissues of Scrapie-affected Sheep with the AA136, QR171 Prion Protein Genotype -
Kunkle, R.A., Hamir, A.N., Richt, J., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2009. Lack of Prion Accumulation in Lymphoid Tissues of Scrapie-affected Sheep with the AA136, QR171 Prion Protein Genotype [abstract]. American Association of Veterinary Laboratory Diagnosticians. Paper No. 186.
Susceptibility of Cattle to First-Passage Intracerebral Inoculation with Chronic Wasting Disease Agent from Elk -
Greenlee, J.J., Nicholson, E.M., Kunkle, R.A., Hamir, A.N. Susceptibility of Cattle to First-Passage Intracerebral Inoculation with Chronic Wasting Disease Agent from Elk [abstract]. American College of Veterinary Pathologists Annual Meeting. Veterinary Pathology. 46(5):1058.
Prolonged Incubation Time in Sheep with QK171 Genotype -
Greenlee, J.J., Nicholson, E.M., Kunkle, R.A., Hamir, A.N. 2009. Prolonged Incubation Time in Sheep with QK171 Genotype [abstract]. Prion 2009. p. 120.
Guanidinium Chloride Unfolding of PrP**Sc Exhibits Marked Differences Between Genotypes -
Nicholson, E.M., Greenlee, J.J., Kunkle, R.A., Hamir, A.N. 2009. Guanidinium Chloride Unfolding of PrP**Sc Exhibits Marked Differences Between Genotypes [abstract]. Prion 2009. p. 182.
Characterization of a U.S. Sheep Scrapie Isolate with Short Incubation Time Reprint Icon -
Hamir, A.N., Richt, J.A., Kunkle, R.A., Greenlee, J.J., Bulgin, M.S., Gregori, L., Rohwer, R.G. 2009. Characterization of a U.S. Sheep Scrapie Isolate with Short Incubation Time. Veterinary Pathology. 46(6):1205-1212.
Origins of BSE: evidence of sporadic and heritable BSE -
Nicholson, E.M., Brunelle, B.W., Kehrli, Jr., M.E., Greenlee, J.J. 2008. Origins of BSE: Evidence of Sporadic and Heritable BSE [abstract]. United States-Japan Cooperative Program in Natural Resources. p. 7.
Experimental transmission of U.S. scrapie agent to neonatal sheep by oral route -
Hamir, A.N., Kunkle, R.A., Greenlee, J.J., Richt, J.A. 2009. Experimental Oral Transmission of United States Origin Scrapie to Neonatal Sheep. Journal of Veterinary Diagnostic Investigation. 21(1):64-68.
Frequencies of polymorphisms associated with BSE resistance differ significantly between Bos taurus, Bos indicus, and composite cattle -
Brunelle, B.W., Greenlee, J.J., Seabury, C.M., Brown II, C.E., Nicholson, E.M. 2008. Frequencies of Polymorphisms Associated with BSE Resistance Differ Significantly Between Bos taurus, Bos indicus, and Composite Cattle. BioMed Central (BMC) Veterinary Research. 4(1):36. Available: http://www.biomedcentral.com/1746-6148/4/36.
Serial passage of sheep scrapie inoculum in Suffolk sheep -
Hamir, A.N., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Miller, J.M. 2009. Serial Passage of Sheep Scrapie Inoculum in Suffolk Sheep. Veterinary Pathology. 46(1):39-44.
Strain typing of U.S. scrapie strains using a panel of inbred mice -
Greenlee, J.J., Kunkle, R.A., Nicholson, E.M., Hamir, A.N., Bulgin, M.S., Richt, J. 2009. Strain Typing of U.S. Scrapie Strains Using a Panel of Inbred Mice [abstract]. American College of Veterinary Pathologists. Paper No. 121. p. 762.
Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy -
Hamir, A.N., Cutlip, R.C., Miler, J.M., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2008. Experimental Transmission of Transmissible Spongiform Encephalopathies (Scrapie, Chronic Wasting Disease, Transmissible Mink Encephalopathy) to Cattle and Their Differentiation from Bovine Spongiform Encephalopathy [abstract]. American College of Veterinary Pathologists. Paper No. 153. p. 770.
Identification of a heritable polymorphism in bovine PRNP associated with genetic transmissible spongiform encephalopathy: evidence of heritable BSE Reprint Icon -
Nicholson, E.M., Brunelle, B.W., Richt, J.A., Kehrli, Jr., M.E., Greenlee, J.J. 2008. Identification of a Heritable Polymorphism in Bovine PRNP Associated with Genetic Transmissible Spongiform Encephalopathy: Evidence of Heritable BSE. PloS ONE. 3(8):e2912.
Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy -
Hamir, A.N., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2008. Experimental Transmission of Transmissible Spongiform Encephalopathies (Scrapie, Chronic Wasting Disease, Transmissible Mink Encephalopathy) to Cattle and their Differentiation from Bovine Spongiform Encephalopathy [abstract]. Prion 2008. p. 65.
Lack of prion accumulation in lymphoid tissues of scrapie-affected sheep with AA136, QR171 polymorphism type -
Kunkle, R.A., Hamir, A.N., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2008. Lack of Prion Accumulation in Lymphoid Tissues of Scrapie-Affected Sheep with AA136, QR171 Polymorphism Type [abtract]. Prion 2008. p. 75.
Identification of a heritable polymorphism in the bovine prion gene associated with genetic transmissible spongiform encephalopathy: evidence of heritable BSE -
Nicholson, E.M., Brunelle, B.W., Richt, J.A., Kehrli, Jr., M.E., Greenlee, J.J. 2008. Identification of a Heritable Polymorphism in the Bovine Prion Gene Associated with Genetic Transmissible Spongiform Encephalopathy: Evidence of Heritable BSE [abstract]. Prion 2008. p. 114.
Western-blot detection of PrP**sc in archived paraffin-embedded brainstem from scrapie-affected sheep -
Kunkle, R.A., Nicholson, E.M., Lebepe-Mazur, S., Orcutt, D.L., Srinivas, M.L., Greenlee, J.J., Alt, D.P., Hamir, A.N. 2008. Western blot detection of PrP**sc in archived paraffin-embedded brainstem from scrapie-affected sheep. Journal of Veterinary Diagnostic Investigation. 20(4):522-526.
Experimental transmission of chronic wasting disease (CWD) of elk (Cervus elaphus nelsoni), white-tailed deer (Odocoileus virginianus), and mule deer (Odocoileus hemionus hemionus) to white-tailed deer by intracerebral route Reprint Icon -
Hamir, A.N., Richt, J., Miller, J.M., Kunkle, R.A., Hall, S., Nicholson, E.M., Orourke, K.I., Greenlee, J.J., Williams, E.S. 2008. Experimental transmission of chronic wasting disease (CWD) of elk (Cervus elaphus nelsoni), white-tailed deer (Odocoileus virginianus), and mule deer (Odocoileus hemionus hemionus) to white-tailed deer by intracerebral route. Veterinary Pathology. 45(3):297-306.
Prolonged CWD incubation time and unique PrP**d profile in Prnp 132LL elk Reprint Icon -
Greenlee, J.J., O'Rourke, K.I., Hamir, A.N., Gidlewski, T., Zhuang, D., Spraker, T. 2007. Prolonged CWD Incubation Time and Unique PrP**d Profile in Prnp 132LL Elk. In: Proceedings of the U.S. Animal Health Association 111th Annual Meeting, Captive Wildlife and Alternative Livestock Committee, October 20, 2007, Reno, Nevada. p. 264-265.
Retinal cell types are differentially affected in sheep with scrapie -
Smith, J.D., Greenlee, J.J., Hamir, A.N., West Greenlee, M.H. 2008. Retinal cell types are differentially affected in sheep with scrapie. Journal of Comparative Pathology. 138(1):12-22.
Elk with a long incubation prion disease phenotype have a unique PrP-d profile Reprint Icon -
O'Rourke, K.I., Spraker, T.R., Zhuang, D., Greenlee, J.J., Gidlewski, T.E., Hamir, A.N. 2007. Elk with a long incubation prion disease phenotype have a unique PrP-d profile. NeuroReport. 18(18):1935-1938.
Experimental transmission of U.S. scrapie agent by nasal, peritoneal and conjunctival routes to genetically susceptible sheep -
Hamir, A.N., Kunkle, R.A., Richt, J.A., Miller, J.M., Greenlee, J.J. 2008. Experimental transmission of US scrapie agent by nasal, peritoneal, and conjunctival routes to genetically susceptible sheep. Veterinary Pathology. 45(1):7-11.
Abnormal prion protein in the retina of Rocky Mountain elk (Cervus Elaphus Nelsoni) Reprint Icon -
Spraker, T, Greenlee, J., Gidlewski T., O’Rourke, K. 2007. Abnormal Prion Protein in the Retina of Rocky Mountain Elk (Cervus Elaphus Nelsoni) [abstract]. Prion 2007. Paper No. P03.70. p. 64.
Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy -
Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Richt, J.A., Greenlee, J.J., Nicholson, E.M., Kehrli, Jr., M.E. 2007. Experimental transmission of transmissible spongiform encephalopathies (scrapie, chronic wasting disease, transmissible mink encephalopathy) to cattle and their differentiation from bovine spongiform encephalopathy. In: Proceedings of the World Association of Veterinary Laboratory Diagnosticians 13th International Symposium, November 11-14, 2007, Melbourne, Australia. p. 29.
Detection of PrP**CWD in retinal tissues in white-tailed deer (Odocoileus virginianus) and Rocky Mountain elk (Cervus elaphus nelsoni) with CWD -
Spraker, T., Gidleweski, T., Greenlee, J., Keane, D., Hamir, A., Orourke, K. 2007. Detection of PrP**CWD in retinal tissues in white-tailed deer (Odocoileus virginianus) and Rocky Mountain elk (Cervus elaphus nelsoni) with CWD [abstract]. American Association of Veterinary Laboratory Diagnosticians 50th Annual Meeting. p. 47.
Cross-species transmission of TSE -
Richt, J.A., Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Greenlee, J.J. 2007. Cross-species transmission of TSE. In: Proceedings of the American College of Veterinary Internal Medicine. 2007 ACVIM Forum, June 6-9, 2007, Seattle, Washington. Paper No. 160.
EXPERIMENTAL TRANSMISSION OF CHRONIC WASTING DISEASE (CWD) OF ELK (CERVUS ELAPHUS NELSONI), WHITE-TAILED DEER (ODOCOILEUS VIRGINIANUS), AND MULE DEER (ODOCOILEUS HEMIONUS HEMIONUS) TO WHITE-TAILED DEER BY INTRACEREBRAL ROUTE -
Kunkle, R.A., Hamir, A.N., Miller, J.M., Hall, S.M., Richt, J.A., Greenlee, J.J., Williams, E.S. 2006. Experimental transmission of chronic wasting disease (CWD) of elk (Cervus Elaphus Nelsoni), white-tailed deer (Odocoileus Virginianus), and mule deer (Odocoileus Hemionus Hemionus) to white-tailed deer by intracerebral route [abstract]. United States Animal Health Association. p. 221.
ATTEMPTED TRANSMISSION OF SCRAPIE AND CWD TO SWINE: PRELIMINARY FINDINGS -
Greenlee, J.J., Kunkle, R.A. Nicholson, E.M., Lager, K.M., Hamir, A.N. 2006. Attempted transmission of scrapie and CWD to swine: preliminary findings. 19th International Pig Veterinary Society Congress. p. 386.
ABNORMAL PRION ACCUMULATION ASSOCIATED WITH RETINAL PATHOLOGY IN EXPERIMENTALLY INOCULATED SCRAPIE-AFFECTED SHEEP -
Greenlee, J.J., Hamir, A.N., West Greenlee, M.H. 2006. Abnormal prion accumulation associated with retinal pathology in experimentally inoculated scrapie-affected sheep. Veterinary Pathology. 43:733-739.
EXPERIMENTAL TRANSMISSION OF TRANSMISSIBLE MINK ENCEPHALOPATHY (TME) TO CATTLE BY INTRACEREBRAL INOCULATION -
Hamir, A.N., Kunkle, R.A., Miller, J.M., Greenlee, J.J., Richt, J.A. 2006. Experimental transmission of transmissible mink encephalopathy (TME) to cattle by intracerebral inoculation [abstract]. 4th International Veterinary Vaccines and Diagnostics Conference. p. 89. Paper No. PO53.
CROSS-SPECIES TSE TRANSMISSION STUDIES AT NADC -
Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Greenlee, J.J., Richt, J.A. 2006. Cross-species TSE transmission studies at NADC [abstract]. 4th International Veterinary Vaccines and Diagnostics Conference. p. 89. Paper No. PO52.
EXPERIMENTAL INTRACEREBRAL AND ORAL INOCULATION OF SCRAPIE TO SWINE: PRELIMINARY REPORT -
Greenlee, J.J., Kunkle, R.A., Hamir, A.N. 2005. Experimental intracerebral and oral inoculation of scrapie to swine: preliminary report [abstract]. Proceedings of the American Association of Veterinary Laboratory Diagnosticians 48th Annual Conference. p. 38.
NATURAL AND EXPERIMENTAL PRION DISEASES OF CATTLE -
Richt, J.A., Hamir, A.N., Kunkle, R.A., Nicholson, E.M., Greenlee, J.J., Miller, J.M., Cutlip, R., Davis, A.J., Hall, S.M. 2005. Natural and experimental prion diseases of cattle [abstract]. Third International Rushmore Conference on Enteric Diseases. Paper No. 14.
EXPERIMENTAL SECOND PASSAGE OF CHRONIC WASTING DISEASE (CWD(MULE DEER)) AGENT TO CATTLE -
Hamir, A.N., Kunkle, R.A., Miller, J.M., Greenlee, J.J., Richt, J.A. 2006. Experimental second passage of chronic wasting disease (CWD(mule deer)) agent to cattle. Journal of Comparative Pathology. 134(1):63-69.
EXPERIMENTAL TRANSMISSION OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES (TSE) AT THE NATIONAL ANIMAL DISEASE CENTER, AMES, IOWA: AN UPDATE -
Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Greenlee, J.J., Richt, J.A. 2005. Experimental transmission of transmissible spongiform encephalopathies (TSE) at the National Animal Disease Center, Ames, Iowa: an update [abstract]. American Association of Veterinary Laboratory Diagnosticians. p. 169.
BIOCHEMISTRY OF PRION DISEASES -
Nicholson, E.M., Greenlee, J.J., Richt, J., Hamir, A.N., Kunkle, R.A., Kehrli, Jr., M.E. 2005. Biochemistry of prion diseases. Association of Analytical Communities International, Midwest Section [abstract]. Abstract No. 601. p. 38.
EXPERIMENTAL TRANSMISSION OF TRANSMISSIBLE SPONGIFORM ENCEPHALOPATHIES (TSES) AT THE NATIONAL ANIMAL DISEASE CENTER (NADC), AMES, IOWA, USA: AN UPDATE -
Hamir, A.N., Cutlip, R.C., Miller, J.M., Kunkle, R.A., Greenlee, J.J., Richt, J.A., Bartz, J. 2005. Experimental transmission of transmissible spongiform encephalopathies (TSEs) at the National Animal Disease Center (NADC), Ames, Iowa, USA: An update [abstract]. Annual Wildlife Disease Association Conference. p. 263.
EXPERIMENTAL CANINE LEPTOSPIROSIS CAUSED BY LEPTOSPIRA INTERROGANS SEROVARS POMONA AND BRATISLAVA -
Greenlee, J.J., Alt, D.P., Bolin, C.A., Zuerner, R.L., Andreasen, C.B. 2005. Experimental canine Leptospirosis caused by Leptospira interrogans serovars Pomona and Bratislava. American Journal of Veterinary Research. 66(10):1816-1822.
LOSS OF PRION PROTEIN IMMUNOREACTIVITY AFTER BOILING IN SATURATED CALCIUM HYDROXIDE SOLUTION -
Greenlee, J.J., Nicholson, E.M., Hamir, A.N., Richt, J.A., Kunkle, R.A., Noyes, G., Hotzapple, M.T., Kehrli Jr., M.E. 2005. Loss of prion protein immunoreactivity after boiling in saturated calcium hydroxide solution [abstract]. Keystone Symposia: Molecular Mechanisms of Transmissible Spongiform Encephalopathies. Poster No. 125.
Last Modified: 5/2/2016
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