Submitted to: PLoS One
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/8/2008
Publication Date: 8/13/2008
Publication URL: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0002912
Citation: Nicholson, E.M., Brunelle, B.W., Richt, J.A., Kehrli, Jr., M.E., Greenlee, J.J. 2008. Identification of a Heritable Polymorphism in Bovine PRNP Associated with Genetic Transmissible Spongiform Encephalopathy: Evidence of Heritable BSE. PloS ONE. 3(8):e2912. Interpretive Summary: Bovine spongiform encephalopathy (BSE) is a prion disease of cattle. Classical BSE is associated with ingestion of BSE contaminated feedstuffs. Atypical BSE is not associated with contaminated feed and is likely a spontaneous disorder. In one animal positive for atypical BSE, a genetic change analogous to the most prevalent form of inherited human prion disease was identified. In order to determine if this change is a heritable trait in cattle we analyzed the only known offspring of this animal. Our analysis revealed that both the atypical BSE animal and its offspring contain the genetic change, and that this heritable trait may exist at a low level in the cattle population. This is the first evidence that BSE may be transmitted genetically in cattle. Also, this provides the first evidence that spontaneous, hereditary and infectious prion diseases exist in a species other than humans.
Technical Abstract: Bovine spongiform encephalopathy (BSE) is a transmissible spongiform encephalopathy (TSE) of cattle. Classical BSE is associated with ingestion of BSE-contaminated feedstuffs. H- and L-type BSE, collectively known as atypical BSE, differ from classical BSE by displaying a different disease phenotype and they have not been linked to the consumption of contaminated feed. Interestingly, the 2006, U.S. H-type atypical BSE animal had a polymorphism at codon 211 of the bovine prion gene resulting in a glutamic acid to lysine substitution (E211K). This substitution is analogous to the most prevalent form of heritable TSE in humans, and it is considered to have caused BSE in the 2006, U.S. atypical BSE animal. In order to determine if this amino acid change is a heritable trait in cattle, we sequenced the prion alleles of the only known offspring of this animal, a 2-year-old heifer. Principal Findings: Sequence analysis revealed that both the 2006, U.S. atypical BSE animal and its 2-year-old heifer were heterozygous at bovine prion gene nucleotides 631 through 633 for GAA (glutamic acid) and AAA (lysine). Both animals carry the E211K polymorphism, indicating that the allele is heritable and may persist within the cattle population. Conclusions: This is the first evidence that the E211K polymorphism is a germline polymorphism, not a somatic mutation, indicating BSE may be transmitted genetically in cattle. This also provides the first evidence that all 3 etiologic forms of TSEs (spontaneous, hereditary, and infectious) are present in a non-human species. Atypical BSE arising as both genetic and spontaneous disease, in the context of reports that at least some forms of atypical BSE can convert to classical BSE in mice, suggests a cattle origin for classical BSE.