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ARS Home » Northeast Area » Orient Point, New York » Plum Island Animal Disease Center » Foreign Animal Disease Research » People & Locations » Douglas Gladue
Douglas Gladue (PhD)
Foreign Animal Disease Research
Research Microbiologist

Phone: (631) 323-3035
Fax: (631) 323-3006

40550 ROUTE 25
ORIENT POINT, NY 11957

(Employee information on this page comes from the REE Directory. Please contact your front office staff to update the REE Directory.)

Projects
Intervention Strategies to Control and Eradicate Foreign Animal Diseases of Swine
In-House Appropriated (D)
  Accession Number: 441308
Exploration Into a Stable Cell Line Capable to Support African Swine Fever Growth
Interagency Reimbursable Agreement (I)
  Accession Number: 434924
Systematic Identification of Non-Essential African Swine Fever Virus Gene Targets for Developing Next Generation Vaccines with Increased Safety
Interagency Reimbursable Agreement (I)
  Accession Number: 436767
African Swine Fever Collaborative Agreement
Non-Funded Cooperative Agreement (N)
  Accession Number: 440732
Systematic Discovery of ASFV Determinants of Virulence to Improve the Safety Profile of First Generation Live-Attenuated ASFV Vaccines
Reimbursable Cooperative Agreement (R)
  Accession Number: 441514
Predictive Structure of African Swine Fever Viral Proteins
Non-Assistance Cooperative Agreement (S)
  Accession Number: 433925
Research Supporting Workforce Development for the National Bio-Agro Defense Facility: Development of CSF Subunit Vaccines
Non-Assistance Cooperative Agreement (S)
  Accession Number: 435093
Analysis of the Interaction Between Virus Proteins and Membrane Structures in Cells Infected with Classical Swine Fever Virus (CSFV)
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436252
Functional Genomics of African Swine Fever Virus Infections - An Effective System to Identify Pro-Tolerance and Resilience Pathways in Pigs
Non-Assistance Cooperative Agreement (S)
  Accession Number: 440094
Evaluation of the Modification of Virulence in Recombinant ASFV with Specific Gene Deletions
Non-Assistance Cooperative Agreement (S)
  Accession Number: 440174
Development of a Self-amplifying mRNA Vaccine for African Swine Fever and Classical Swine Fever
Non-Assistance Cooperative Agreement (S)
  Accession Number: 440187
Identification of Critical Host Genes for African Swine Fever Virus Infection Using Genome-Scale CRISPR Knock-Out Cell Libraries
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436791
Actions Supporting the Development of an African Swine Fever Virus Live Attenuated DIVA Vaccine
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436793
Genetic Characterization of African Swine Fever Virus Causing Disease Outbreaks in Asia and Africa
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436802
Development of an African Swine Fever Virus Subunit Vaccine
Non-Assistance Cooperative Agreement (S)
  Accession Number: 436806
Characterizing the Structure-Function of Various Families of Viroporins to Support African Swine Fever Virus Vaccine Candidates
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438233
Development of Synthetic African Swine Fever Viruses to Determine the Role of Structural Viral Proteins
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438304
CRISPR KO and CRISPRa Libraries for Creating Stable Cell Lines for Use in African Swine Fever Virus Vaccines
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438358
Identification of Host Proteins That Interact with ASFV Proteins Through Yeast Two Hybrid Screening
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438473
Analysis of the Immune Response Elicited by Classical Swine Fever Virus Recombinant Vaccine Candidate FlagT4G
Non-Assistance Cooperative Agreement (S)
  Accession Number: 438599
Evaluation of Lyophilization on efficacy of African Swine Fever Vaccine Candidate ASFV-G-delta I177L
Trust Fund Cooperative Agreement (T)
  Accession Number: 438734

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometry Reprint Icon - (Peer Reviewed Journal)
Canter, J.A., Aponte, T., Ramirez Medina, E., Pruitt, S.E., Neilan, J., Gladue, D.P., Borca, M.V., Zhu, J.J. 2022. Determining hyperimmune serum neutralizing effect on African Swine Fever Virus and serum enhancing effect on extracellular virion infectivity in adherent pig PBMC by flow cytometry. Scientific Reports. https://doi.org/10.3390/v14061249.
World society for virology first international conference: tackling global virus epidemics Reprint Icon - (Review Article)
Soderlund-Venermo, M., Varma, A., Guo, D., Gladue, D.P., Poole, E., Pujol, F.H., Pappu, H., Romalde, J.L., Kramer, L., Baz, M., Venter, M., Moore, M.D., Nevels, M.M., Ezzikouri, S., Vakharia, V.N., Wilson, W.C., Malik, Y., Shi, Z., Abdel-Moneim, A.S. 2021. World society for virology first international conference: tackling global virus epidemics. Virology. 566(2022):114-121. https://doi.org/10.1016/j.virol.2021.11.009.
Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez Salinas, L., Pina-Pedrero, S., Zhu, J.J., Rodriguez, F., Borca, M.V., Gladue, D.P. 2021. Deletion of E184L, a Putative DIVA Target from the Pandemic Strain of African Swine Fever Virus, Produces a Reduction in Virulence and Protection against Virulent Challenge. Viruses. https://doi.org/10.1128/JVI.01419-21.
Additional deletions to further attenuate BA71¿CD2 recombinant virus decreases vaccine efficacy without providing additional safety. - (Peer Reviewed Journal)
Development of a novel African swine fever virus (ASFV) live attenuated vaccine candidate: deletion of A137R gene drastically attenuates Georgia strain and induce protection against the virulent challenge Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., Ramirez Medina, E., Vuono, E., Silva, E.B., Rai, A., Pruitt, S.E., Espinoza, N.N., Velazquez-Salinas, L., Borca, M.V. 2021. Development of a novel African swine fever virus (ASFV) live attenuated vaccine candidate: deletion of A137R gene drastically attenuates Georgia strain and induce protection against the virulent challenge. Journal of Virology. https://doi.org/10.1128/JVI.01139-21.
Evaluation of the function of ASFV KP177R gene, encoding for structural protein p22, in the process of virus replication and in swine virulence Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E., Pruitt, S.E., Rai, A., Espinoza, N.N., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2021. Evaluation of the function of ASFV KP177R gene, encoding for structural protein p22, in the process of virus replication and in swine virulence. Viruses. https://doi.org/10.3390/v13060986.
A cell culture-adapted vaccine virus against the current pandemic African swine fever virus strain Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Rai, A., Ramirez-Medina, E., Silva, E.B., Velazquez-Salinas, L., Vuono, E., Espinoza, N.N., Pruitt, S.E., Gladue, D.P. 2021. A cell culture-adapted vaccine virus against the current pandemic African swine fever virus strain. Nature Communications. https://doi.org/10.1128/JVI.00123-21.
ASFV-G-I177L is an effective oral nasal vaccine against the Eurasia Strain of Africa swine fever Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Ramirez Medina, E., Silva, E., Vuono, E., Rai, A., Pruitt, S.E., Gay, C.G., Espinoza, N.N., Velazquez-Salinas, L., Gladue, D.P. 2021. ASFV-G-I177L is an effective oral nasal vaccine against the Eurasia Strain of Africa swine fever. Viruses. https://doi.org/10.3390/v13050765.
Structural glycoprotein E2 of classical swine fever virus critically interacts with host protein Torsin-1A during the virus infectious cycle Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez-Media, E., Velazquez-Salinas, L., Berggren, K., Rai, A., Pruitt, S.E., Espinoza, N.N., Gladue, D.P., Borca, M.V. 2021. Structural glycoprotein E2 of classical swine fever virus critically interacts with host protein Torsin-1A during the virus infectious cycle. Virology Journal. https://doi.org/10.1128/JVI.00314-21.
Evaluation of the deletion of ASFV MGF110-5L-6L on swine virulence and its potential use as a DIVA vaccine marker gene Reprint Icon - (Peer Reviewed Journal)
Ramirez Medina, E., Vuono, E., Pruitt, S.E., Rai, A., Valladares, A., Espinoza, N.N., Velazquez Salinas, L., Gladue, D.P., Borca, M.V. 2021. Evaluation of the deletion of ASFV MGF110-5L-6L on swine virulence and its potential use as a DIVA vaccine marker gene. Viruses. https://doi.org/10.3390/v13020286.
Development and in vivo evaluation of a recombinant African swine fever strain Georgia with a deletion in the MGF110-1L gene Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Silva, E., Espinoza, N.N., Velazquez-Salinas, L., Zhu, J.J., Borca, M.V., Gladue, D.P. 2021. Development and in vivo evaluation of a recombinant African swine fever strain Georgia with a deletion in the MGF110-1L gene. Viruses. https://doi.org/10.3390/v13020286.
Evaluation in swine of a recombinant Georgia 2010 African Swine Fever Virus lacking the I8L Gene Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez-Medina, E., Rai, A., Pruitt, S.E., Silva, E., Espinoza, N.N., Velazquez-Salinas, L., Zhu, J.J., Borca, M.V., Gladue, D.P. 2020. Evaluation in swine of a recombinant Georgia 2010 African Swine Fever Virus lacking the I8L Gene. Viruses. https://doi.org/10.3390/v13010039.
Deletion of CD2-like (CD2v) and C-type lectin-like (EP153R) genes from African swine fever virus Georgia- ¿9GL abrogates its effectiveness as an experimental vaccine Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., O'Donnell, V., Ramierez-Medina, E., Rai, A., Pruitt, S.E., Vuono, E., Silva, E., Velazquez-Salinas, L., Borca, M.V. 2020. Deletion of CD2-like (CD2v) and C-type lectin-like (EP153R) genes from African swine fever virus Georgia- 9GL abrogates its effectiveness as an experimental vaccine. Viruses. https://doi.org/10.3390/v12101185.
Evaluation in swine of a recombinant African swine fever virus lacking the MGF-360-1L gene Reprint Icon - (Peer Reviewed Journal)
Ramierez-Medina, E., Vuono, E., Rai, A., Pruitt, S.E., Silva, E., Velazquez-Salinas, L., Zhu, J.J., Gladue, D.P., Borca, M.V. 2020. Evaluation in swine of a recombinant African swine fever virus lacking the MGF-360-1L gene. Viruses. https://doi.org/10.3390/v12101193.
Positive Selection of ORF1ab, ORF3a, and ORF8 Genes Drives the Early Evolutionary Trends of SARS-CoV-2 During the 2020 COVID-19 Pandemic Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Zarate, S., Eberl, S., Gladue, D.P., Novella, I., Borca, M.V. 2020. Positive selection of ORF1ab, ORF3a, and ORF8 genes drives the early evolutionary trends of SARS-CoV-2 during the 2020 COVID-19 pandemic. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2020.550674.
X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine. Viruses. https://doi.org/10.3390/v12090918.
X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S.E., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. X69R is a non-essential gene that when deleted from African swine fever does not affect virulence in swine. Viruses. https://doi.org/10.3390/v12090918.
The C962R ORF of African swine fever strain Georgia is non-essential and not required for virulence in swine Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E.A., Pruitt, S., Rai, A., Silva, E., Zhu, J.J., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. The C962R ORF of African swine fever strain Georgia is non-essential and not required for virulence in swine. Viruses. https://doi.org/10.3390/v12060676.
Residues within the foot-and-mouth disease virus 3dpol nuclear localization signal affect polymerase fidelity Reprint Icon - (Peer Reviewed Journal)
Kloc, A., Rai, D.K., Kenney, M.A., Schafer, E.A., Rieder, A.E., Gladue, D. 2020. Residues within the foot-and-mouth disease virus 3dpol nuclear localization signal affect polymerase fidelity. Journal of Virology. https://doi.org/10.1128/JVI.00833-20.
A Single Amino Acid Substitution In The Matrix Protein (M51R) Of Vesicular Stomatitis New Jersey Virus Impairs Replication In Cultured Porcine Macrophages And Results In Significant Attenuation In Pigs Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Pauszek, S.J., Holinka, L.G., Gladue, D.P., Rekant, S.I., Bishop, E.A., Stenfeldt, C., Verdugo-Rodriguez, A., Borca, M.V., Rodriguez, L.L., Arzt, J. 2020. A single amino acid substitution in the matrix protein (M51R) of Vesicular Stomatitis New Jersey virus impairs replication in cultured porcine macrophages and results in significant attenuation in pigs. Frontiers in Microbiology. https://doi.org/10.3389/fmicb.2020.01123.
SERTA domain containing protein 1 (SERTAD1) interacts with classical swine fever virus structural glycoprotein E2 which is involved in virus virulence in swine Reprint Icon - (Peer Reviewed Journal)
Vuono, E., Ramirez-Medina, E., Azzinaro, P.A., Berggren, K., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Borca, M.V., Gladue, D.P. 2020. SERTA domain containing protein 1 (SERTAD1) interacts with classical swine fever virus structural glycoprotein E2 which is involved in virus virulence in swine. Journal of Virology. https://doi.org/10.3390/v12040421.
Detection and quantification of African swine fever in MA104 cells Reprint Icon - (Peer Reviewed Journal)
Rai, A., Pruitt, S.E., Ramierez-Medina, E., Vuono, E., Silva, E., Velazquez-Salinas, L., Carrillo, C., Gladue, D.P., Borca, M.V. 2020. Detection and quantification of African swine fever in MA104 cells. Bio-protocol. https://doi.org/10.21769/BioProtoc.3955.
Swine host protein coiled-coil domain containing 115 (CCDC115) interacts with classical swine fever virus structural glycoprotein E2 during virus Reprint Icon - (Peer Reviewed Journal)
Vuonno, E., Ramirez-Media, E., Berggren, K., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2020. Swine host protein coiled-coil domain containing 115 (CCDC115) interacts with classical swine fever virus structural glycoprotein E2 during virus. Viruses. https://doi.org/10.3390/v12040388.
The MGF360-16R ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host proteins SERTAD3 and SDCBP Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Silva, E., Rai, A., Pruitt, S.E., Vuono, E.A., Velazquez-Salinas, L., Berggren, K.A., Zhu, J.J., Borca, M.V., Gladue, D.P. 2020. The MGF360-16R ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host proteins SERTAD3 and SDCBP. Viruses. https://doi.org/10.1128/JVI.02017-19.
Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine. Reprint Icon - (Abstract Only)
Borca, M.V., Ramirez Media, E., Silva, E., Vuono, E., Rai, A., Pruitt, S., Holinka, L., Velazquezsalinas, L., Gladue, D.P., Risatti, G., Shi, J. 2020. Deletion of CD2-like gene from the genome of African swine fever virus strain Georgia does not attenuate virulence in swine.. Meeting Abstract. https://doi.org/10.1038/s41598-020-57455-3.
First report describing the spatial-temporal circulation and the genetic diversity of Parapoxvirus species in Mexico Reprint Icon - (Other)
Velazquezsalinas, L., Ramirez Media, E., Bracht, A., Carrillo, C., Gladue, D.P. 2019. First report describing the spatial-temporal circulation and the genetic diversity of Parapoxvirus species in Mexico. Atlas of Science. https://doi.org/10.1016/j.meegid.2018.07.005.
Mechanisms of African Swine Fever Virus Pathogenesis and Immune Evasion Inferred from Gene Expression Changes in Infected Swine Macrophages - (Peer Reviewed Journal)
Structural glycoprotein E2 of classical swine fever virus binding to host protein dynactin subunit (DCTN6) is necessary for virus virulence in swine Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Vuono, E.A., Ramirez-Medina, E., Azzinaro, P.A., Berggren, K.A., Singer, M., Rai, A., Pruitt, S., Silva, E., Velazquez-Salinas, L., Carrillo, C., Gladue, D.P. 2019. Structural glycoprotein E2 of classical swine fever virus binding to host protein dynactin subunit (DCTN6) is necessary for virus virulence in swine. Journal of Virology. https://doi.org/10.1128/JVI.01642-19.
The differential effect of the deletion of African swine fever virus virulence-associated genes in the induction of attenuation of the highly virulent Georgia strain Reprint Icon - (Peer Reviewed Journal)
Ramirez-Medina, E., Vuono, E., O'Donnell, V., Holinka, L.G., Silva, E., Rai, A., Pruitt, S., Carrillo, C., Gladue, D.P., Borca, M.V. 2019. The differential effect of the deletion of African swine fever virus virulence-associated genes in the induction of attenuation of the highly virulent Georgia strain. Virology. https://doi.org/10.3390/v11070599.
Interaction of structural glycoprotein E2 of Classical Swine Fever Virus with protein phosphatase 1 catalytic subunit beta (PP1ß) - (Peer Reviewed Journal)
Vuono, E., Ramirez-Medina, E., Holinka-Patterson, L.G., Baker-Branstetter, R., Borca, M.V., Gladue, D.P. 2019. Interaction of structural glycoprotein E2 of Classical Swine Fever Virus with protein phosphatase 1 catalytic subunit beta (PP1ß). Virology. Viruses 2019, 11(4), 307. https://doi.org/10.3390/v11040307.
Identification of structural glycoprotein E2 domain critical to mediate Classical Swine Fever Virus replication in SK6 cells Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Holinka-Patterson, L.G., Ramirez-Medina, E., Vuono, E., Berggren, K., Gladue, D.P. 2018. Identification of structural glycoprotein E2 domain critical to mediate Classical Swine Fever Virus replication in SK6 cells. Virology. 526:38-44. https://doi.org/10.1016/j.virol.2018.10.004.
Molecular characterization of the viroporin function of foot-and-mouth disease virus non-structureal protein 2B Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., Largo, E., De La Arada, I., Aguilella, V., Alcaraz, A., Arrondo, J., Holinka-Patterson, L.G., Brocchi, E., Ramirez-Medina, E., Vuono, E., Berggren, K., Carrillo, C., Borca, M.V. 2018. Molecular characterization of the viroporin function of foot-and-mouth disease virus non-structureal protein 2B. Journal of Virology. https://doi.org/10.1128/JVI.01360-18.
Classical swine fever virus p7 protein interacts with host protein CAMLG and regulates calcium permeability at the endoplasmic reticulum Reprint Icon - (Peer Reviewed Journal)
Gladue, D.P., Largo, E., Holinka-Patterson, L.G., Ramirez-Medina, E., Vuono, E.A., Berggren, K., Risatti, G.R., Nieva, J.L., Borca, M.V. 2018. Classical swine fever virus p7 protein interacts with host protein CAMLG and regulates calcium permeability at the endoplasmic reticulum. Virus Research. 10(9):E460. https://doi.org/10.3390/v10090460.
CRISPR/Cas gene editing of a large DNA virus: African Swine Fever Virus Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Berggren, K., Ramirez, E., Vuono, E., Gladue, D.P. 2018. CRISPR/Cas gene editing of a large DNA virus: African Swine Fever Virus. Bio-protocol. 8(16). https://doi.org/10.21769/BioProtoc.2978.
Phylodynamics of parapoxvirus genus in Mexico (2007–2011) Reprint Icon - (Peer Reviewed Journal)
Velazquez-Salinas, L., Ramirez-Medina, E., Bracht, A.J., Hole, K., Brito, B., Gladue, D.P., Carillo, C. 2018. Phylodynamics of parapoxvirus genus in Mexico (2007–2011). Infection, Genetics and Evolution. https://doi.org/10.1016/j.meegid.2018.07.005 .
The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host protein IL-1ß Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Ramirez, E., Vuono, E., Berggren, K., Alfano, M., Carey, L.B., Richt, J.A., Risatti, G.R., Gladue, D.P. 2018. The L83L ORF of African swine fever virus strain Georgia encodes for a non-essential gene that interacts with host protein IL-1ß. Virus Research. 249:116-123. https://doi.org/10.1016/j.virusres.2018.03.017.
CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., Holinka-Patterson, L.G., Berggren, K., Gladue, D.P. 2018. CRISPR-Cas9, a tool to efficiently increase the development of recombinant African swine fever viruses. Scientific Reports. 8(3154). https://doi.org/10.1038/s41598-018-21575-8.
A partial deletion within foot-and-mouth disease virus non-structural protein 3A causes clinical attenuation in cattle but does not prevent subclinical infection Reprint Icon - (Peer Reviewed Journal)
Stenfeldt, C., Arzt, J., Pacheco, J., Gladue, D.P., Smoliga, G.R., Silva, E., Rodriguez, L.L., Borca, M.V. 2018. A partial deletion within foot-and-mouth disease virus non-structural protein 3A causes clinical attenuation in cattle but does not prevent subclinical infection. Virology. 516:115-126. https://doi.org/10.1016/j.virol.2018.01.008.
Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses Reprint Icon - (Peer Reviewed Journal)
Largo, E., Gladue, D.P., Aguilella, V.M., Alcaraz, A., Borca, M.V., Nieva, J.L. 2018. Mutation-induced changes of transmembrane pore size revealed by combined ion-channel conductance and single vesicle permeabilization analyses. Biochimica et Biophysica Acta. 1860:1015-1021. https://doi.org/10.1016/j.bbamem.2018.01.012.
Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G - (Peer Reviewed Journal)
Holinka-Patterson, L.G., O'Donnell, V., Risatti, G., Azzinaro, P.A., Arzt, J., Stenfeldt, C., Velazquez-Salinas, L., Gladue, D.P., Borca, M.V. 2017. Early protection events in swine immunized with an experimental live attenuated classical swine fever marker vaccine, FlagT4G. PLoS One. doi: 10.1371/journal.pone.0177433.
Development of a fluorescent ASFV strain that retains the ability to cause disease in swine - (Peer Reviewed Journal)
Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Sanford, B., Azzinaro, P.A., Risatti, G., Gladue, D.P. 2017. Development of a fluorescent ASFV strain that retains the ability to cause disease in swine. Nature Scientific Reports. 7:46747. doi: 10.1038/srep46747.
Simultaneous deletion of the 9GL and UK genes from the African swine fever virus Georgia 2007 isolate results in virus attenuation and may be a potential virus vaccine strain - (Peer Reviewed Journal)
O'Donnell, V., Risatti, G.R., Holinka-Patterson, L.G., Krug, P.W., Carlson, J., Velazquez-Salinas, L., Azzinaro, P., Gladue, D.P., Borca, M.V. 2017. Simultaneous deletion of the 9GL and UK genes from the African swine fever virus Georgia 2007 isolate results in virus attenuation and may be a potential virus vaccine strain. Journal of Virology. 91(1):e01760-16.
Association of the host immune response with protection using a live attenuated African swine fever virus model Reprint Icon - (Peer Reviewed Journal)
Carlson, J., O'Donnell, V.K., Alfano, M., Velazquez-Salinas, L., Holinka-Patterson, L.G., Krug, P.W., Gladue, D.P., Higgs, S., Borca, M.V. 2016. Association of the host immune response with protection using a live attenuated African swine fever virus model . Viruses. 8(10):E291. https://doi.org/10.3390/v8100291.
Association of the host immune response with protection using a live attenuated African swine fever virus model - (Peer Reviewed Journal)
Carlson, J., O'Donnell, V., Alsfonso, M., Velazquez Salinas, L., Holinka-Patterson, L.G., Krug, P.W., Gladue, D.P., Higgs, S., Borca, M.V. 2016. Association of the host immune response with protection using a live attenuated african swine fever virus model. Viruses. 8(10):291. doi: 10.3390/v8100291.
Alteration of a second putative fusion peptide of structural glycoprotein E2 of Classical Swine Fever Virus alters virus replication and virulence in swine - (Peer Reviewed Journal)
Holinka-Patterson, L.G., Largo, E., Gladue, D.P., O'Donnell, V.K., Risatti, G.R., Nieva, J.L., Borca, M.V. 2016. Alteration of a second putative fusion peptide of structural glycoprotein E2 of Classical Swine Fever Virus alters virus replication and virulence in swine. Journal of Virology. 90:10299-10308. doi: 10.1128/JVI.01530-16.
The Ep152R ORF of African Swine Fever Virus strain Georgia encodes for an essential gene that interacts with host protein BAG6 Reprint Icon - (Peer Reviewed Journal)
Borca, M.V., O'Donnell, V., Holinka-Patterson, L.G., Rai, D., Sanford, B., Alfano, M., Carlson, J., Azzinaro, P., Alonso, C., Gladue, D.P. 2016. The Ep152R ORF of African Swine Fever Virus strain Georgia encodes for an essential gene that interacts with host protein BAG6. Virus Research. 223:181-189. doi: 10.1016/j.virusres.2016.07.013.
Selective factors associated with the evolution of codon usage in natural populations of arboviruses and their practical application to infer possible hosts for emerging viruses - (Peer Reviewed Journal)
Velazquez-Salinas, L., Zarate, S., Eschbaumer, M., Lobo, F.P., Gladue, D.P., Arzt, J., Novella, I.S., Rodriguez, L.L. 2016. Selective factors associated with the evolution of codon usage in natural populations of arboviruses and their practical application to infer possible hosts for emerging viruses. PLoS One. PLoS ONE 11(7): e0159943. doi:10.1371/journal.pone.0159943.
African Swine Fever Virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes in highly attenuated in swine but does not confer protection against parental virus challenge - (Peer Reviewed Journal)
O'Donnell, V.K., Holinka-Patterson, L.G., Gladue, D.P., Sanford, B., Krug, P.W., Carlson, J., Pacheco, J., Reese, B., Risatti, G.R., Borca, M.V. 2016. African Swine Fever Virus Georgia isolate harboring deletions of 9GL and MGF360/505 genes in highly attenuated in swine but does not confer protection against parental virus challenge. Virology. 221:8-14. doi: 10.1016/j.virusres.2016.05.014.
Recoding structural glycoprotein E2 in classical swine fever virus (CSFV) produces complete virus attenuation in swine and protects infected animals against disease - (Peer Reviewed Journal)
Velazquez-Salinas, L., Risatti, G.R., Holinka-Patterson, L.G., O'Donnell, V.K., Carlson, J., Alfano, M., Rodriguez, L.L., Carrillo, C., Gladue, D.P., Borca, M.V. 2016. Recoding classical swine fever virus (CSFV) structural glycoprotein E2 produces complete virus attenuation in swine and protects infected animals against disease. Virology. 494:178-189. doi: 10.1016/j.virol.2016.4.007.
Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus - (Peer Reviewed Journal)
Sandford, B., Holinka-Patterson, L.G., O'Donnell, V., Krug, P.W., Carlson, J., Alfano, M., Carrillo, C., Wu, P., Lowe, A., Risatti, G.R., Gladue, D.P., Borca, M.V. 2015. Deletion of the thymidine kinase gene induces complete attenuation of the Georgia isolate of African swine fever virus. Virus Research. 213:165-171. doi: 10.1016/j.viruses.2015.12.002.
Interaction between core protein of classical swine fever virus with cellular IQGAP1 proetin appears essential for virulence in swine - (Peer Reviewed Journal)
Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., O'Donnell, V.K., Vepkhvadze, N., Lu, Z., Risatti, G.R., Borca, M.V. 2011. Interaction between core protein of classical swine fever virus with cellular IQGAP1 proetin appears essential for virulence in swine. Virology. 412:68-74.
Identification of an NTPase motif in classical swine fever virus NS4B protein - (Peer Reviewed Journal)
Gladue, D.P., Gavrilov, B.K., Holinka-Patterson, L.G., Fernandez-Sainz, I.J., Vepkhvadze, N.G., Rogers, K., O'Donnell, V., Borca, M.V. 2011. Identification of an NTPase motif in classical swine fever virus NS4B protein. Virology. 411(1):41-49.
Effects of the interactions of classical swine fever virus core protein with proteins of SUMOylation pathway on virulence in swine - (Peer Reviewed Journal)
Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Saniz, I., Prarat, M.V., O'Donnell, V.K., Vepkhvadze, N., Lu, Z., Rogers, K., Risatti, G.R., Borca, M.V. 2010. Effects of the interactions of classical swine fever virus core protein with proteins of SUMOylation pathway on virulence in swine. Virology. 407:129-136.
Substitution of specific cysteine residues in E1 glycoprotein of classical swine fever virus strain Brescia affects formation of E1-E2 heterodimers and alters virulence in swine - (Peer Reviewed Journal)
Fernandez-Sainz, I.J., Holinka-Patterson, L.G., Gladue, D.P., O'Donnell, V., Lu, Z., Gavrilov, B.K., Risatti, G.R., Borca, M.V. 2011. Substitution of specific cysteine residues in E1 glycoprotein of classical swine fever virus strain Brescia affects formation of E1-E2 heterodimers and alters virulence in swine. Journal of Virology. 407:129-136.
Mutations in the classical swine fever virus NS4B protein affects virulence in swine - (Abstract Only)
Fernandez Sainz, I., Gladue, D.P., Holinka-Patterson, L.G., O'Donnell, V.K., Gudmundsdottir, I., Prarat, M.V., Patch, J.R., Golde, W.T., Zhiqiang, L., Risatti, G.R., Zhu, J.J., Borca, M.V. 2009. Mutations in the classical swine fever virus NS4B protein affects virulence in swine. Proceedings. P. 143.
Mutations in classical swine fever virus NS4B affect virulence in swine - (Peer Reviewed Journal)
Fernandez-Sainz, I., Gladue, D.P., Holinka-Patterson, L.G., O'Donnell, V.K., Gudmundsdottir, I., Prarat, M.V., Patch, J.R., Golde, W.T., Lu, Z., Risatti, G.R., Zhu, J.J., Borca, M.V. 2010. Mutations in classical swine fever virus NS4B affect virulence in swine. Journal of Virology. 84(3):1536-1549.
Sumoylation of the Core Protein in Classical Swine Fever Virus is Essential for Virulence in swine - (Abstract Only)
Gladue, D.P., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., Lu, Z., Borca, M.V. 2009. Sumoylation of the Core Protein in Classical Swine Fever Virus is Essential for Virulence in swine. American Society for Virology Meeting. Paper No. W49-5: P. 213.
Alteration of the N-linked Glycosylation Condition of E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Alters Virulence in Swine - (Peer Reviewed Journal)
Fernandez-Sainz, I.J., Holinka-Patterson, L.G., Gavrilov, B.K., Prarat, M.V., Gladue, D.P., Lu, Z., Jia, W., Risatti, G.R., Borca, M.V. 2009. Alteration of the N-linked Glycosylation Condition of E1 Glycoprotein of Classical Swine Fever Virus Strain Brescia Alters Virulence in Swine. Virology. 386:210-216.
Development of a live attenuated antigenic marker classical swine fever vaccine - (Peer Reviewed Journal)
Holinka-Patterson, L.G., Fernandez-Sainz, I., O'Donnell, V., Prarat, M.V., Gladue, D.P., Lu, Z., Risatti, G., Borca, M.V. 2008. Development of a live attenuated antigenic marker classical swine fever vaccine. Virology. 384:106-113.
Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray - (Peer Reviewed Journal)
Gladue, D.P., Zhu, J.J., Holinka-Patterson, L.G., Fernandez-Sainz, I., Prarat, M.V., O'Donnell, V.K., Borca, M.V. 2010. Patterns of gene expression in swine macrophages infected with classical swine fever virus detected by microarray. Virology. 138(1-2):89-96.
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