Submitted to: Veterinary Immunology and Immunopathology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/12/1997
Publication Date: N/A
Interpretive Summary: This manuscript presents the summary of the first round of testing results from the Second International Swine CD Workshop. This workshop was organized so scientists worldwide could independently verify the reactivity of monoclonal antibodies (mAbs) reactive with pig immune cells. Use of such well-characterized mAb reagents would eanble scientists internationally to readily compare data as they analysed swine disease and vaccine responses. A total of 176 mAb were submitted by laboratories around the world for the workshop. Together with 19 previously characterized swine CD internal standards the full panel of 195 mAb was analyzed by flow cytometry on 18 different cell types. Statistical clustering analyses resulted in 22 clusters of mAb. These clusters of mAb were assigned to specific cell subsets, e.g., T cells, B cells, macrophages, and were then distributed to laboratories for more extensive testing by specific groups of scientists who specialized in each swine immune cell subset. Firm characterization of mAb which recognize unique swine immune cell CD determinants will enable scientists to clearly differentiate unique subsets of swine T and B cells and to assess fully the functions of these important lymphoid cell subpopulations. This international workshop enabled scientists worldwide to precisely identify important new reagents to aid analyses of swine immunity.
Technical Abstract: The reactivity of 176 monoclonal antibodies (mAb) submitted to the Second International Swine CD Workshop together with 19 internal standards was analyzed by flow cytometry on 18 different cell types as a means of establishing the proper cell subset for later detailed clustering analyses. The exact CD subset reactivity of the 19 internal standard mAb had been characterized in the First International Swine CD Workshop. The flow cytometric analyses revealed in 40 data sets which were then subjected to statistical clustering using the Leukocyte Typing Database IV (LTDB4) software. As a result of this work 22 clusters were defined. After review of these results, panels of mAb from the defined first round clusters were asigned to cell subsets. The respective mAb in those first round clusters were then distributed to subset group researchers for further examination during the second round of the workshop.