Submitted to: Association Official Analytical Chemists Annual Intrl Meeting & Exposition
Publication Type: Abstract Only
Publication Acceptance Date: 9/11/1997
Publication Date: N/A
Technical Abstract: Supercritical fluid-based analytical techniques and their resultant methods have provided an environmentally-acceptable technology which minimizes the use of organic solvents in the chemical laboratory. Our laboratory has played a seminal role in conceptualizing and reducing to practice, CO2-based methods for trace pesticide analysis, the determination of nutrient content of foods, and the extraction of mycotoxins from a variety of sample matrices. Developmental efforts have benefitted in many cases from principles initially developed in the area of process SF technology; particularly in the application of SFE for fat analysis. This lecture will trace the logic sequence which has guided the development of analytical SFE and its coupling with such techniques as sorbent trapping, SPE, enzyme immunoassay, and sample cleanup or chemical reactions sequenced with the extraction step. We have found that proper scaling of the extraction experiment,as well as preparation of the sample matrix, are critical to the success of SFE, and these aspects of SFE, along with adjunct SF-based techniques such as inverse SFE, size exclusion chromatography, and hyphenated variants SFE GC/SFE-GC MS/SFE-SFC, will be noted in this presentation. Finally, the status of SF-based analytical techniques with respect to official methods, commercial instrumentation, and future research opportunities will conclude the lecture.