Submitted to: Supercritical Fluids International Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 3/31/1996
Publication Date: N/A
Technical Abstract: A method was developed by Jackson and King for the synthesis of fatty acid methyl esters (FAMEs) using sequential supercritical fluid extraction and enzyme reaction (SFE/SFR) of soybean. Canola seeds, soybeans, sunflower seeds, and wheat germ were used to study the effect of moisture. The samples were handled in three ways prior to SFE/SFR: no pre treatment; freeze-dried; and addition of Hydromatrix. Extractions were performed on an automated system using an SFE unit, connected on-line with a GC. The SFE/SFR conditions were: 2500 psi, 50C, CO2 flow rate of 1 mL/min and methanol of 5uL/min. The FAMEs were collected onto a trap; followed by a hexane rinse into a vial. A robotic arm from the GC placed the vial in the autoinjector of the GC for FAME analysis. Fatty acid composition, total fat, saturated fat, and monounsaturated fat content were determined from the FAMEs. Total fat content was greater for the freeze-dried samples and lowest from the control samples for all four oilseeds studied. The total fat content from the samples with Hydromatrix fell between the values of the freeze-dried and control values, except wheat germ with Hydromatrix was the same as the control untreated sample. Saturated and monounsaturated fat content tend to follow the same trend as the total fat content data. Fatty acid composition from all four oilseed samples agreed with values determined by traditional methods.