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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Poultry Microbiological Safety and Processing Research Unit » Research » Publications at this Location » Publication #381273

Research Project: Production and Processing Intervention Strategies for Poultry Associated Foodborne Pathogens

Location: Poultry Microbiological Safety and Processing Research Unit

Title: Differences in Campylobacter growth in a novel medium in flasks incubated aerobically or anaerobically

item Hinton Jr, Arthur
item Cox Jr, Nelson
item LEVICAN, ARTURO - Pontifical Catholic University Of Valparaiso

Submitted to: World Poultry Congress Proceedings
Publication Type: Abstract Only
Publication Acceptance Date: 5/12/2021
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Campylobacter are major foodborne pathogens associated with poultry products. These bacteria require carbon dioxide (CO2) for growth and are usually cultured in containers incubated in artificially produced, microaerobic atmospheres. The objective of this research was to examine the growth of the pathogen in a novel medium in containers incubated aerobically or anaerobically. A medium composed of beef extract, tryptose, soluble starch, sodium bicarbonate, sodium lactate, and agar was prepared then inoculated with log 4.0 cfu/ml of Campylobacter coli, Campylobacter fetus, Campylobacter jejuni, or Campylobacter lari. Media was transferred to flasks with vented caps, vented caps covered with Parafilm, or plug-sealed caps; then incubated at 37C for 48 h in aerobic (21% 02, 0.04% CO2, 78% N2) or anaerobic (10% H2, 5% CO2, 85% N2) atmospheres. Campylobacter were then enumerated, and significant differences in the number of cfu recovered were determined using GraphPad InStat statistical software. Results indicated that Campylobacter growth was dependent on type of flask closure and incubation atmosphere. There was no significant (p < 0.05) difference the growth of different Campylobacter species incubated in flasks with the same closures when incubated in the same atmosphere. Although, no Campylobacter were recovered from vented flasks incubated aerobically, 4-5 log cfu Campylobacter/ml were recovered from vented flasks incubated anaerobically. Also, significantly more Campylobacter (log 7-8 log cfu Campylobacter/ml) were recovered from Parafilm-covered flasks and plugged flasks than from vented flasks incubated aerobically or anaerobically. Other studies have shown that relative CO2 concentrations in the flasks were vented