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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Poultry Microbiological Safety and Processing Research Unit » Research » Publications at this Location » Publication #366095

Research Project: Novel Pre-harvest Interventions and Alternatives to Antibiotics to Reduce Foodborne Pathogens

Location: Poultry Microbiological Safety and Processing Research Unit

Title: Seroprevalence in Broilers against Two Important Foodborne Pathogens Salmonella and Campylobacter jejuni

item Yeh, Hung-Yueh

Submitted to: International Veterinary Immunology Symposium
Publication Type: Abstract Only
Publication Acceptance Date: 5/15/2019
Publication Date: N/A
Citation: N/A

Interpretive Summary: none

Technical Abstract: Background: Poultry production is one of important components in agricultural output worldwide. It is critical that higher quality and microbiologically safer poultry products be produced for human consumption. Outbreaks of human salmonellosis and campylobacteriosis have often been linked to consumption and handling of unsanitary poultry products. Both Salmonella and Campylobacter jejuni are regarded as commensals in the broiler gastrointestinal tracts. It was demonstrated that maternal antibodies against whole cell lysates of these bacteria were detected. Additionally, the antibodies in sera against these two bacteria could be detected in naturally infected poultry flocks. However, antigenicity of individual proteins using broiler sera has not been extensively studied. The aim of this study was to explore the antigenicity of flagellar and chemotactic proteins to identify potential novel antigens for broilers. Methods: The genes of flagella and chemotactic proteins of Campylobacter jejuni and Salmonella were amplified by PCR. The amplicons were ligated into a pETite vector and transformed into E. coli cells. The proteins were over-expressed in Escherichia coli cells harboring the genes. The recombinant proteins were purified by a nickel-chelating affinity chromatography, and confirmed by SDS-PAGE analysis and the His tag detection. The recombinant proteins were tested for their antigenicity with immunoblot using chicken sera from several geographical locations.