Location: Poultry Microbiological Safety and Processing Research UnitTitle: Different combinations of enrichment and plating media for detection of naturally occurring campylobacter from retail chicken livers
|Cox, Nelson - Nac|
|Hinton, Jr, Arthur|
|Plumblee Lawrence, Jodie|
Submitted to: International Poultry Scientific Forum
Publication Type: Abstract Only
Publication Acceptance Date: 12/19/2018
Publication Date: 7/15/2019
Citation: Thompson, T.M., Cox Jr, N.A., Berrang, M.E., Hinton Jr, A., Yeh, H., Plumblee Lawrence, J.R., Brooks, S.Q. 2019. Different combinations of enrichment and plating media for detection of naturally occurring campylobacter from retail chicken livers [abstract]. International Poultry Scientific Forum. 98(1):63.
Interpretive Summary: none
Technical Abstract: Foodborne campylobacteriosis has been traced to undercooked chicken liver. The objective of this study was to compare the effectiveness of two liquid media: Bolton (B) and Neogen (N) formulation and three solid plating media: RF Campylobacter agar (RF), Campy Cefex agar (CCA) and Campy Line agar (CLA) for recovery of Campylobacter from chicken liver. Twenty-seven replicate plastic tubs of liver were purchased at retail outlets. From each tub, three samples of exudate and five intact liver lobes were collected. Exudate samples (0.1 mL) were direct streaked onto 3 separate plates (RF, CCA and CLA). An additional 1 mL of exudate was transferred into 9 mL each type of enrichment broth. Each lobe was rinsed in 100 mL of saline for 60 s; 10 mL of rinsate was transferred into 90 mL of B and another 10 mL into 90 mL of N. All plates and tubes were incubated at 42' for 48 h in re-sealable bags flushed with microaerophilic gas. After incubation, enriched samples were streaked on RF, CCA and CLA plates and were similarly incubated. Following incubation, 3 typical colonies were selected from each plate and confirmed to be Campylobacter by observation of cellular morphology and motility under phase contrast microscopy. There were a total of 117 direct plated samples for each plating medium. Campylobacter recovery was compared using Chi Square test for independence. Campylobacter was detected from 30.8%, 29.1% and 28.2% of CLA, RF and CCA, respectively, for an overall 29.3 % positive samples. For enriched exudate samples, media combination did not have a significant effect on Campylobacter recovery (P>0.05). Recovery rate ranged from 77.8% (49/63) for the combination of B to RF to 65.1% (41/63) for N to CLA; overall recovery for enriched weep was 73%. Likewise, the combination of media used for enriched rinse did not significantly affect recovery which ranged from N to RF had the highest recovery rate at 76.2% (48/63) for N to RF to 63.5% (40/63) for B to CLA. Neither did plating medium have a significant effect on recovery from directly plated rinses which ranged from 22.2% (12/54) for CCA to 14.8% (8/54) for CLA. More than 50% of chicken livers purchased at retail are Campylobacter positive and choice of recovery media did not influence detection.