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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Poultry Microbiological Safety & Processing Research » Research » Publications at this Location » Publication #356491

Research Project: Genomic and Proteomic Approaches for the Characterization of Foodborne Pathogens in Poultry

Location: Poultry Microbiological Safety & Processing Research

Title: Community-level physiological profiling for microbial community function in broiler cecae

Author
item Yeh, Hung-yueh
item Line, John - Eric
item Hinton, Jr, Arthur

Submitted to: Current Microbiology
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 11/19/2018
Publication Date: N/A
Citation: N/A

Interpretive Summary: Poultry production is an important agricultural output worldwide. It is known that the gut health of broilers is essential for their growth and for providing wholesome products for human consumption. Previously, the microbial diversity of broiler ceca was studied at the genetic level. However, the functional diversity and metabolic activity of broiler cecal bacterial communities are not fully investigated. Recently, the EcoPlates™ from Biolog, Inc. have been used for characterizing bacterial communities from various environments. In this communication, we applied these plates to physiologically profile cecal bacterial communities in broilers. The ceca were aseptically excised from six-week-old broilers, and their contents were suspended in phosphate buffered saline. The cultures in the EcoPlates were incubated at 42 oC for five days in an OmniLog system. Responses of the bacterial communities to the various substrates were measured on formazin production. The results show the sigmoidal growth curves with three phases were observed in all 12 cecal samples. Cecal bacterial communities could not use 11 carbon substrates for carbon sources, while they used pyruvic acid methyl ester, glycogen, glucose-1-phosphate and N-acetyl-D-glucosamine the most. Each bacterial community metabolized various numbers of the substrates at different rates among broilers. In the future, modification of the culture conditions mimic to the gut environment is needed. More investigations on the effects of nutrients, Salmonella or Campylobacter on physiological functions of cecal bacterial communities will provide insights into the improvement of animal well-being, saving production expenditure for producers and providing safer poultry products for human consumption.

Technical Abstract: Poultry production is one of major agricultural output worldwide. It is known that the gut health of broilers is essential for their growth and for providing wholesome products for human consumption. Previously, the microbial diversity of broiler ceca was studied at the genetic level. However, the functional diversity and metabolic activity of broiler cecal bacterial communities are not fully investigated. Recently, the EcoPlates™ from Biolog, Inc. have been used for characterizing bacterial communities from various environments. In this communication, we applied these plates to physiologically profile cecal bacterial communities in broilers. The ceca were aseptically excised from six-week-old broilers, and their contents were suspended in phosphate buffered saline. The cultures in the EcoPlates were incubated at 42 oC for five days in an OmniLog system. Responses of the bacterial communities to the various substrates were measured on formazin production. The results show the sigmoidal growth curves with three phases were observed in all 12 cecal samples. Cecal bacterial communities could not use 11 carbon substrates for carbon sources, while they used pyruvic acid methyl ester, glycogen, glucose-1-phosphate and N-acetyl-D-glucosamine the most. Each bacterial community metabolized various numbers of the substrates at different rates among broilers. In the future, modification of the culture conditions mimic to the gut environment is needed. More investigations on the effects of nutrients, Salmonella or Campylobacter on physiological functions of cecal bacterial communities will provide insights into the improvement of animal well-being, saving production expenditure for producers and providing safer poultry products for human consumption.