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ARS Home » Southeast Area » Athens, Georgia » U.S. National Poultry Research Center » Poultry Microbiological Safety & Processing Research » Research » Publications at this Location » Publication #352009

Research Project: Production and Processing Intervention Strategies for Poultry Associated Foodborne Pathogens

Location: Poultry Microbiological Safety & Processing Research

Title: Choice of pre-enrichment media may influence recovery of salmonella from environmental poultry samples

Author
item Cox, Nelson - Nac
item RICHARDSON, KURT - Anitox Corp
item Cosby, Douglas
item Berrang, Mark
item Hinton, Jr, Arthur

Submitted to: Feedstuffs
Publication Type: Popular Publication
Publication Acceptance Date: 4/1/2018
Publication Date: 5/7/2018
Citation: Cox Jr, N.A., Richardson, K.E., Cosby, D.E., Berrang, M.E., Hinton Jr, A. 2018. Choice of pre-enrichment media may influence recovery of salmonella from environmental poultry samples. Feedstuffs. 90(5).

Interpretive Summary: none

Technical Abstract: The first step to detect Salmonella in feed and other dry contaminated environmental poultry samples is a pre-enrichment broth. This is done to assist in the recovery of small numbers of stressed cells. However, when these samples contain fermentable substrates and a plethora of extraneous bacteria, acidity in certain pre-enrichment media is produced resulting in a low pH that can injure or kill the target organism. When broiler litter and turkey boot covers were analyzed in this study using lactose broth pH values below 5.0 were observed and this can prevent the recovery of Salmonella. Many researchers analyzing these type of samples with certain pre-enrichment media are totally unaware of the hostile acidity that results and are therefore underestimating the presence of Salmonella in these samples. Commonly used methods for these type of samples should be re-evaluated and improved to prevent or minimize false negative results.