|GE, LIANGFA - Samuel Roberts Noble Foundation, Inc
|YU, JIANBIN - Kansas State University
|LUTH, DIANE - Iowa State University
|WANG, KAN - Iowa State University
|CHEN, RUJIN - Samuel Roberts Noble Foundation, Inc
Submitted to: Proceedings of the National Academy of Sciences (PNAS)
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/7/2016
Publication Date: 10/17/2016
Publication URL: https://handle.nal.usda.gov/10113/5695373
Citation: Ge, L., Yu, J., Wang, H., Luth, D., Bai, G., Wang, K., Chen, R. 2016. Increasing seed size and quality by manipulating BIG SEEDS 1 in legume species. Proceedings of the National Academy of Sciences. doi:10.1073/pnas.1611763113.
Interpretive Summary: Seed size is one of the major agronomic traits that contributes to crop yield. We identified a novel gene, BIG SEEDS1 (BS1), from the species Medicago truncatula, which is a close relative of alfalfa. We found that this gene controls seed size and weight both in Medicago truncatula and soybean . BS1 encodes a plant-specific transcription regulator and plays a key role in controlling the size of seeds, seed pods and leaves, through regulating primary cell proliferation in legumes. This research provides a new strategy for the increase of seed yield and quality in legumes.
Technical Abstract: Plant organs such as seeds are primary sources of food for both humans and animals. Seed size is one of the major agronomic traits that have been selected in crop plants during their domestication. Legume seeds are a major source of dietary proteins and oils. Here, we report a novel and conserved role for BIG SEEDS1 (BS1) gene in the control of seed size and weight in the model legume Medicago truncatula and the grain legume soybean (Glycine max). BS1 encodes a plant-specific transcription regulator and plays a key role in the control of the size of plant organs, including seeds, seed pods and leaves, through a regulatory module that targets primary cell proliferation. Importantly, down-regulation of BS1 orthologs in soybean by an artificial microRNA significantly increased soybean seed size, weight and amino acid content. Our results provide a new strategy for the increase of yield and seed quality in legumes.