|Hinton, Jr, Arthur|
|Ingram, Kimberly - Kim|
|TAHA, ENSAF - Tuskegee University|
Submitted to: Southern Poultry Science Society Meeting Abstracts
Publication Type: Abstract Only
Publication Acceptance Date: 11/20/2014
Publication Date: 1/26/2015
Citation: Hinton Jr, A., Gamble, G.R., Ingram, K.D., Taha, E., Holser, R.A. 2015. Inhibition of Salmonella Typhimurium by Cultures of Cecal Bacteria during Aerobic Incubation. Southern Poultry Science Society Meeting Abstracts. Meeting Abstract.
Technical Abstract: Two trials were conducted to examine the ability of cecal bacterial cultures from broilers to inhibit growth of Salmonella Typhimurium during aerobic incubation. Cecal broth media was inoculated with 10 µl of cecal contents from 6 week old broilers taken from 2 separate flocks. Cultures were incubated aerobically at 37oC for 48 h. Supplemented cecal media was prepared by the addition of: #1) 0 mM ethanol, lactate, and succinate; #2) 104 mM ethanol and 50 mM lactate and succinate; #3) 208 mM ethanol and 100 mM lactate and succinate; or #4) 312 mM ethanol and 150 mM lactate and succinate. Each medium was inoculated with 0.25 ml of the cecal culture, 104 cfu/ml of Salmonella, or the cecal culture and Salmonella. Inoculated media were incubated aerobically at 37oC for 21 days, and aliquots of media were collected on days 0, 7, 14, and 21 for analysis. Cecal bacteria and Salmonella were enumerated, and cecal colonies were selected for identification by the Biolog Bacterial Identification System. Results from Trials 1 and 2 indicated that after 21 days of incubation, between log 6 and 7 cfu/ml of Salmonella were recovered from all media inoculated with Salmonella only. Conversely, in Trial 1 after 21 days, log 4 to 5 cfu/ml Salmonella were recovered from all media inoculated with Salmonella and cecal cultures. However in Trial 2, no Salmonella were recovered on days 14 or 21 from supplemented medium #4 inoculated with Salmonella and cecal cultures. Furthermore, log 0.74, 2.27, and 4.67 more Salmonella were recovered from Trial 1 than Trial 2 from supplemented media #2, 3, and 4, respectively, inoculated with Salmonella and cecal cultures. Cecal isolates were identified primarily as Enterococcus spp. in addition to Proteus mirabilis, Bacillus lentus, Paenibacillus wynnii, Streptococcus gallolyticus, and several isolates not identified by the Biolog. Findings indicate that cecal cultures incubated aerobically may possess anti-Salmonella activity related to the ability to utilize metabolites produced by intestinal bacteria. However, cultures from different flocks may vary in their ability to inhibit the growth of Salmonella; therefore, all cecal cultures may not be suitable sources of bacterial isolates required to formulate effective, defined probiotic cultures.