Page Banner

United States Department of Agriculture

Agricultural Research Service

Research Project: Genetic Improvement of Hard Winter Wheat to Biotic and Abiotic Stresses

Location: Hard Winter Wheat Genetics Research

Title: An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley)

Author
item Lui, Hui
item Bayer, Micha
item Druka, Arnis
item Russell, Joanne
item Hackett, Christine
item Poland, Jesse
item Ramsay, Luke
item Hedley, Pete
item Waugh, Robbie

Submitted to: Biomed Central (BMC) Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/3/2014
Publication Date: 2/6/2014
Publication URL: http://www.biomedcentral.com/1471-2164/15/104
Citation: Lui, H., Bayer, M., Druka, A., Russell, J., Hackett, C., Poland, J.A., Ramsay, L., Hedley, P., Waugh, R. 2014. An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley. Biomed Central (BMC) Genomics. 15:104.

Interpretive Summary: Genetic mapping of traits in crop plants has been limited by the high cost of identifying suitable molecular markers. Genotyping-by-Sequencing (GBS) is a newly developed genotyping approach that uses next-generation sequencing to detect new high-density markers in populations of interest. We explored the use of GBS on a population derived from a cross between the two-rowed barley cultivar ‘Golden Promise’ and six-rowed cultivar ‘Morex’ to map the dwarfing gene, Ari-e. By connecting measurements of plant height to the genetic marker data, the Ari-e gene was mapped to a small region on chromosome 5H. We demonstrated that GBS was an effective and relatively low-cost approach to rapidly construct a high-density genetic map in barley. The GBS mapping approach should be useful in many other crop species as well.

Technical Abstract: We explored the use of genotyping by sequencing (GBS) on a recombinant inbred line population (GPMx) derived from a cross between the two-rowed barley cultivar ‘Golden Promise’ (ari-e.GP/Vrs1) and the six-rowed cultivar ‘Morex’ (Ari-e/vrs1) to map plant height. We identified three Quantitative Trait Loci (QTL), the first in a region encompassing the spike architecture gene Vrs1 on chromosome 2H, the second in an uncharacterised centromeric region on chromosome 3H, and the third in a region of chromosome 5H coinciding with the previously described dwarfing gene Breviaristatum-e (Ari-e).

Last Modified: 8/24/2016
Footer Content Back to Top of Page