Location: Hard Winter Wheat Genetics ResearchTitle: An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley) Author
Submitted to: Biomed Central (BMC) Genomics
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 2/3/2014
Publication Date: 2/6/2014
Publication URL: http://www.biomedcentral.com/1471-2164/15/104
Citation: Lui, H., Bayer, M., Druka, A., Russell, J., Hackett, C., Poland, J.A., Ramsay, L., Hedley, P., Waugh, R. 2014. An evaluation of genotyping by sequencing (GBS) to map the Breviaristatum-e (ari-e) locus in cultivated barley. Biomed Central (BMC) Genomics. 15:104. Interpretive Summary: Genetic mapping of traits in crop plants has been limited by the high cost of identifying suitable molecular markers. Genotyping-by-Sequencing (GBS) is a newly developed genotyping approach that uses next-generation sequencing to detect new high-density markers in populations of interest. We explored the use of GBS on a population derived from a cross between the two-rowed barley cultivar ‘Golden Promise’ and six-rowed cultivar ‘Morex’ to map the dwarfing gene, Ari-e. By connecting measurements of plant height to the genetic marker data, the Ari-e gene was mapped to a small region on chromosome 5H. We demonstrated that GBS was an effective and relatively low-cost approach to rapidly construct a high-density genetic map in barley. The GBS mapping approach should be useful in many other crop species as well.
Technical Abstract: We explored the use of genotyping by sequencing (GBS) on a recombinant inbred line population (GPMx) derived from a cross between the two-rowed barley cultivar ‘Golden Promise’ (ari-e.GP/Vrs1) and the six-rowed cultivar ‘Morex’ (Ari-e/vrs1) to map plant height. We identified three Quantitative Trait Loci (QTL), the first in a region encompassing the spike architecture gene Vrs1 on chromosome 2H, the second in an uncharacterised centromeric region on chromosome 3H, and the third in a region of chromosome 5H coinciding with the previously described dwarfing gene Breviaristatum-e (Ari-e).