Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer reviewed journal
Publication Acceptance Date: 8/30/2013
Publication Date: 3/27/2014
Publication URL: http://handle.nal.usda.gov/10113/58831
Citation: Sapozhnikova, Y.V., Lobato, A., Romig, B. 2014. Effects of UV-B radiation levels on concentrations of phytosterol, ergothioneine, and polyphenolic compounds in mushroom powder used as dietary supplements. Journal of Agricultural and Food Chemistry. dx.doi.org/10.1021/jf403852k. Interpretive Summary: Mushrooms exposed to UV-B radiation produce elevated levels of vitamin D, and the dried powder is used as a dietary supplement to provide nutritional benefits. However, it is unknown if other chemicals (both beneficial and toxic) are also produced. This study showed that UV-B radiation used to elevate vitamin D levels in mushrooms did not alter compositional changes of investigated bioactive compounds. These results suggest that mushroom powder dietary supplements not only provide vitamin D, but also may be a good source of phytosterols and the naturally occurring mushroom anti-oxidant ergothioneine.
Technical Abstract: Compositional changes of powder dietary supplement made from mushrooms previously exposed to different levels of UV-B irradiation were evaluated for the bioactive naturally occurring mushroom anti-oxidant, ergothioneine, other natural polyphenolic anti-oxidants: e.g. flavonoids, lignans, and others, and selected phytosterols. Four types of mushroom powder consisting of white, brown (Agaricus bisporus), oyster (Pleurotus ostreatus) and shiitake (Lentinula edodes) mushrooms from three different treatment groups (control, low and high UV-B exposures) were analyzed. Ergothioneine concentrations found in mushroom powder were 0.4-10.4 mg/g dry weight (dw), and were not affected by UV-B radiation. No polyphenolic compounds were detected. Ergosterol concentrations were 2.4-6.2 mg/g dw. As expected, ergosterol concentrations decreased with the increased level of UV-B treatment for all, but white mushrooms. Campesterol concentrations were 14-43 µg/g dw, and were not affected by the UV-B radiation. This study showed that UV-B radiation used to elevated vitamin D2 levels in mushrooms did not alter compositional changes of investigated bioactive compounds: anti-oxidant ergothioneine and phytosterol campesterol in mushroom powder dietary supplement.