|PAUL, PIERCE - The Ohio State University|
|QU, FENG - The Ohio State University|
|Redinbaugh, Margaret - Peg|
|MIAO, HONG-QIN - Hebei Academy Of Agriculture & Forestry|
Submitted to: Plant Disease
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/29/2013
Publication Date: 8/1/2013
Citation: Stewart, L.R., Paul, P., Qu, F., Redinbaugh, M.G., Miao, H., Todd, J.C., Jones, M.W. 2013. Wheat mosaic virus (WMoV), the causal agent of High Plains disease, is present in Ohio wheat fields. Plant Disease. 97:1125.
Interpretive Summary: High Plains disease in wheat is caused by a mite-transmitted virus and results in stunting, yellowing, and yield loss in infected fields in the United States. Up until now, the virus was only reported in High Plains states between Nebraska and Idaho. In a survey of Ohio wheat viruses, we identified the virus causing High Plains disease in Ohio, much farther east than reported previously.
Technical Abstract: Wheat mosaic virus (WMoV), the causal agent of High Plains disease in wheat, was found in wheat fields in three western counties in Ohio: Auglaize, Miami, and Paulding. WMoV nucleoprotein sequence was identified from Illumina deep sequencing of RNA collected from symptomatic and asymptomatic wheat samples collected in 14 Ohio counties. Presence of virus in samples from three counties was confirmed by WMoV-specific reverse-transcriptase polymerase chain reaction (RT-PCR) and protein A sandwich enzyme-linked immunosorbent assay (PAS-ELISA). Virus from WMoV-positive samples was transmitted to 2/378 surviving 'Spirit' corn seedlings infected by vascular puncture inoculation (VPI) and showed flecking mosaic symptoms.