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ARS Home » Plains Area » Fargo, North Dakota » Edward T. Schafer Agricultural Research Center » Sugarbeet and Potato Research » Research » Publications at this Location » Publication #280296

Title: Wounding induces expression of genes involved in tuber closing layer and wound-periderm development

item Neubauer, Jonathan
item Lulai, Edward
item THOMPSON, ASUNTA - North Dakota State University
item Suttle, Jeffrey
item Bolton, Melvin

Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 3/30/2012
Publication Date: 2/8/2013
Citation: Neubauer, J., Lulai, E.C., Thompson, A.L., Suttle, J.C., Bolton, M.D. 2013. Wounding induces expression of genes involved in tuber closing layer and wound-periderm development. American Journal of Potato Research. 90:142.

Interpretive Summary:

Technical Abstract: Little is known about the coordinate induction of genes that may be involved in important wound-healing events. In this study, wound-healing events were determined together with wound-induced expression profiles of selected cell cycle, cell wall protein, and pectin methyl esterase genes using tubers from two diverse potato genotypes; NDTX4271-5R and Russet Burbank, 2008 and 2009 harvests. By 5 d after wounding, the closing layer and a nascent phellogen had formed. Phellogen cell divisions generated phellem layers until cessation of cell division at 28 d after wounding for both genotypes. Cell cycle genes were induced by 1 d after wounding; these expressions coordinated with related phellogen formation and the induction and cessation of phellem cell formation. Genes encoding structural cell wall proteins, extensins, were dramatically up-regulated by 1 d to 5 d after wounding, suggesting involvement with closing layer and later phellem cell layer formation. Wounding up-regulated pectin methyl esterase genes; StPME expression increased during closing layer and phellem cell formation, whereas maximum expression of StPrePME occurred at 5 d to 14 d after wounding, implicating involvement in later modifications for closing layer and phellem cell wall formation. The coordinate induction and expression profile of StTLRP, a gene encoding a cell wall strengthening “tyrosine-and lysine-rich protein,” suggested a role in the formation of the closing layer followed by phellem cell generation, but then uniquely extending into periderm maturation. The expression profiles of these wound-inducible genes markedly coordinated with wound-healing events, but were more influenced by harvest than genotype.