|GAJJAR, PRIYANKA - University Of Idaho
|GUPTA, SANJAY - University Of Idaho
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2011
Publication Date: 8/6/2011
Citation: Gajjar, P., Gupta, S.K., Glynn, M.T., Suttle, J.C. 2011. Differential expression of the enzymes associated with cold-induced sweetening in long term cold stored potatoes [abstract.] Plant Biology 2011. Abstract #P08008. Available: http://abstracts.aspb.org/pb2011/public/P08/P08008.html.
Technical Abstract: Accumulation of high levels of reducing sugars during cold storage (4-6°C) known as cold-induced sweetening (CIS) is a major post-harvest disorder and is one of the most significant concerns for the potato processing industry. The biochemical process of reducing sugar accumulation during cold storage involves main four enzymes namely UDP-glucose pyrophosphorylase (UGPase), sucrose phosphate synthase, pyrophosphatase and acid invertase. Past research has identified two key enzymes UGPase and acid invertase directly involved in the regulation of reducing sugar formation. Isoforms of UGPase with distinct kinetic properties related to CIS resistance have been reported. Involvement of the anaerobic respiration pathway enzymes in reducing sugar accumulation has been reported to explain the process of CIS but all the reports do not sufficiently explain the process of CIS resistance. The aim of the present study was to determine the roles of various enzymes in conferring resistance to CIS by evaluating their expression level and isozyme profiles in cold sweetening sensitive (CSS) and resistant (CSR) varieties stored at 3°C and 7°C storage temperatures. Total soluble proteins from CSS and CSR potato cultivars were analyzed using 1D and 2D gel electrophoresis. Proteins were characterized by mass spectrometer and scored against potato protein databases. The electrophoretic pattern varied significantly between the two groups. There were changes in the levels of expression of protein involved in the conversion of starch to sugar in tubers. All the potato varieties having resistance to CIS have A-II isozyme of UGPase coupled with low acid invertase activity. Poster Categories: P08 (Environment Stress – Cold)