Skip to main content
ARS Home » Research » Publications at this Location » Publication #149031

Title: USE OF BACILLUS AND PAENIBACILLUS PRODUCED BACTERIOCINS FOR THERAPEUTIC CONTROL OF CAMPYLOBACTER JEJUNI IN CHICKENS

Author
item SVETOCH, EDWARD - STATE RES CTR, RUSSIA
item Stern, Norman
item ERUSLANOV, BORIS - ST RES CTR, RUSSIA
item KOVALEV, YURI - ST RES CTR, RUSSIA
item VOLODINA, LARISA - ST RES CTR, RUSSIA
item PERELYGIN, VLADIMIR - ST RES CTR, RUSSIA
item MITSEVICH, EVGENI - ST RES CTR, RUSSIA
item MITSEVICH, IRINA - ST RES CTR, RUSSIA
item POKHILENKO, VICTOR - ST RES CTR, RUSSIA
item BORZENKOV, VALERY - ST RES CTR, RUSSIA

Submitted to: Campylobacter Helicobacter and Related Organisms International Workshop
Publication Type: Abstract Only
Publication Acceptance Date: 6/1/2003
Publication Date: 9/10/2003
Citation: Svetoch, E.A., Stern, N.J., Eruslanov, B.V., Kovalev, Y.N., Volodina, L.I., Perelygin, V.V., Mitsevich, E.V., Mitsevich, I.P., Pokhilenko, V.D., Borzenkov, V.N. 2003. Use of bacillus and paenibacillus produced bacteriocins for therapeutic control of campylobacter jejuni in chickens. Campylobacter Helicobacter and Related Organisms International Workshop.

Interpretive Summary:

Technical Abstract: Bacteriocins from Bacillus and Paenibacillus, described in the companion Abstract, were used in therapeutic treatment for chicks colonized by Campylobacter jejuni. In separate experiments, chicks were orally challenged and colonized at 1 or 2 days post-hatch with challenges of ~2 X 106 cfu C. jejuni (strains L4 and B1), and were placed in groups within separate isolation units. Birds were provided ad-libitum access to standard starter feed and water until 3 days before sampling when the therapeutic feeds were administered. Therapeutic feeds consisted of purified individual bacteriocins (0.25 or 0.5 g) micro-encapsulated into 1.25 g polyvinylpyrrolidone and mixed with 100 g cracked corn subsequently extended in 900 g of chicken feed. Some examples of results are, in trial I with 7 day old birds, 5 of 5 control birds were colonized at a mean level of 106.14 cfu/g while 3 of 10 birds in the treated group had mean levels of 101.31 cfu/g; in trial II with 10 day old birds, 5 of 5 control birds were colonized at 108.99 cfu/g while 2 of 10 treated birds had means of <10 cfu/g; in trial III with 9, 11 and 14 day old chicks, 5 of 5 control birds were colonized at mean levels of 108.26, 109.00, and 108.91, cfu/g respectively, while 3 of 9, 2 of 8 and 0 of 9 treated birds had mean levels of 101.33, 100.63, and <1 cfu/g respectively; and in trial IV with 17 day old chicks 10 of 10 control birds were colonized at a mean level of 107.58 cfu/g while none of 16 chicks within either treated group were colonized by Campylobacter when treated with either 0.25 g or 0.5g/KG of feed for 72 hours. Therapeutic treatment consistently reduced both levels and frequency of chick colonization.