Submitted to: Euroresidue Conference Proceedings
Publication Type: Proceedings
Publication Acceptance Date: 4/1/2000
Publication Date: 9/1/2000
Citation: Crooks, S.R.H., Ferguson, J., Baxter, G.A., Fodey, T.L., Brandon, D.L., Elliott, C.T. 2000. Multiresidue analysis of benzimidazole anthelmintics by immuno-biosensor. In: van Ginkel, L.A., Ruiter, A., editors. EuroResidue IV: Residues of veterinary drugs in foods. May 8-10, 2000, Veldhoven, The Netherlands. pp. 322-327. Interpretive Summary: Animal products are routinely screened for the presence of residues of anti-worm drugs, including a group of drugs known as benzimidazoles. A screening procedure for these compounds was developed, based on a technology called an immunobiosensor. Like conventional immunoassays, this technique uses antibodies to detect the drug residues, but has the added advantage of high throughput analysis and generation of results in real time. With further development of sample preparation strategies, this technique will represent a significant stride toward simplification of drug residue analysis.
Technical Abstract: An immuno-biosensor assay was developed for multiresidue screening of benzimidazole compounds in bovine liver. Polyclonal antibodies which bind at least 7 of the major benzimidazoles or their metabolites were raised in sheep after inoculation with a methyl 5(6)- [(carboxypentyl)-thio]-2-benzimidazolecarbamate protein conjugate. A sample preparation procedure in which liver sample homogenates were extracted into ethyl acetate was developed. The limit of detection of the assay (mean +/- 3 sd), calculated from the analysis of 10 known negative liver samples, was 8.4 ng/g. Intra- and inter-assay coefficients of variation were determined as 14.6 and 14.3% respectively, using a negative bovine liver sample fortified with 1000 ng/g albendazole.