|Smith, Timothy - Tim|
|Kappes, Steven - Steve|
Submitted to: Meeting Abstract
Publication Type: Abstract Only
Publication Acceptance Date: 5/16/1999
Publication Date: N/A
Citation: Casas, E., Keele, J.W., Shackelford, S.D., Smith, T.P., Koohmaraie, M., Kappes, S.M., Stone, R.T. 1999. Quantitative trait loci on bovine chromosomes 15 and 29 evidence that human chromosome 11 harbors orthologous genes associated with longissimus tenderness in bovine. From Jay Lush to Genomics: Visions for Animal Breeding and Genetics., Iowa State University, Ames, IA. p. 153. (Abstract) Interpretive Summary:
Technical Abstract: Meat tenderness is an economically important trait in the beef industry. The objective was to identify that human chromosome 11 (HSA11) potentially harbors orthologues to genes that underlie quantitative trait loci (QTL) for meat tenderness located on bovine chromosomes 15 (BTA15) and 29 (BTA29). Two families were developed from Brahman x Hereford (BH; n=294) and Piedmontese x Angus (PA; n=209) sires, to identify genomic regions associated with growth and carcass traits. Longissimus tenderness, measured as Warner-Bratzler shear force (kg), was obtained at 2 (WBS2) and 14 (WBS14) d postmortem in the BH family and at 3 (WBS3) and 14 d postmortem in the PA family. In the BH family, a QTL for WBS14 was identified (P<.00005; expected number of false positives=.05) and located at 28 cM from the most centromeric marker on BTA15. This QTL interacted signifi- cantly with slaughter group, and the difference between progeny with the Brahman paternally inherited allele versus those with Hereford was 1.19 phenotypic standard deviations for one slaughter group. In the PA family, evidence suggesting a QTL for WBS3 and WBS14 was identified (P<.002; expected number of false positives=1) and located between 54 cM and 62 cM from the most centromeric marker on BTA29. The difference between progeny inheriting the Piedmontese paternal allele versus those inheriting the Angus allele was .86 standard deviations for WBS3 and .65 standard de- viations for WBS14. A single nucleotide polymorphism was developed from partial cDNA bovine sequence of mu-calpain and mapped at 54 cM from the most centromeric marker on BTA29, making it a candidate gene for the identified QTL. Other loci should be responsible for the expression of meat tenderness on BTA15 and are likely to be orthologues to loci on HSA11.