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Brenda S Oppert

Research Molecular Biologist


Dr. Brenda Oppert                

Dr. Brenda Oppert
Research Molecular Biologist

USDA-ARS-CGAHR-SPIERU
ATTN: Brenda Oppert
1515 College Avenue
Manhattan, KS  66502

brenda.oppert@usda.gov
Telephone: 785.776.2780
Fax: 785.537.5584
www.ars.usda.gov/pa/cgahr/spieru/oppert


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RESEARCH INTERESTS

Dr. Oppert received B.S. and M.S. degrees in biology from the University of Texas at El Paso. She received a Ph.D. in the area of protein biochemistry from Kansas State University in 1991. Since that time, Dr. Oppert has worked at CGAHR in the area of insect gut biochemistry and functional genomics.
   Dr. Oppert’s research historically has focused on how stored product insects digest food, looking for enzymes/proteins that are critical for survival as potential targets for new control products. Additionally, she has studied mode of action and resistance mechanisms to Bt toxins in stored product moths and beetles. Since 2007, she has applied high throughput sequencing to functional genomics of stored product insects. Using transcriptome sequencing and bioinformatics, Dr. Oppert applies gene expression analysis (RNASeq) to the evaluation of toxin mode of action and insecticide resistance in Tribolium castaneum and Tenebrio molitor. She also uses RNASeq in developmental stages and tissues to identify potential targets for RNAi and CRISPR in stored product pests. She leads projects sequencing the genomes of Rhyzopertha dominica, T. molitor, and Sitophilus oryzae.
   Recently, Dr. Oppert began working on genomic resources for the development of insects as food to meet the global protein sustainability crisis. Working with All Things Bugs LLC, she collaborated on a Department of Defense DARPA SBIR project to develop genetic resources for T. molitor, Gryllodes sigillatus, and Acheta domesticus. The research will improve the protein and vitamin content in insects for animal feed, and solve problems associated with disease in crickets and other species.

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RECENT PUBLICATIONS
Elpidina, E. N., T. A. Semashko, Y. A. Smirnova, E. A. Dvoryakova, Y. E. Dunaevsky, M. A. Belozersky, M. V. Serebryakova, E. V. Klyachko, A. O Abd El-latif, B. Oppert, and I. Y. Filippova. 2019. Direct detection of cysteine peptidases for MALDI-TOF MS analysis using fluorogenic substrates. Anal. Biochem. 567: 45-50.
Hubhachen, Z., H. Jiang, D. Schlipalius, Y. Park, R. N. C. Guedes, B. Oppert, G. Opit, and T. W. Phillips. 2019. A CAPS marker for determination of strong phosphine resistance in Tribolium castaneum from Brazil. J. Pest Sci. p. 1-8.
Oppert, B., F.-C. Chu, S. Reyna, S. Pinzi, S. Adrianos, L. Perkin, and M. Lorenzen. 2019. Effects of targeting eye color in Tenebrio molitor through RNA interference of tryptophan 2,3-dioxygenase (vermilion): Implications for insect farming. Arch. Insect Biochem. Physiol. 10: e21546.
Perkin, L. C., and B. Oppert. 2019. Gene expression in Tribolium castaneum life stages: identifying a species-specific target for pest control applications. Peer J 7: e6946.
Adrianos, S., M. Lorenzen, and B. Oppert. 2018. Metabolic pathway interruption: CRISPR/Cas9-mediated knockout of tryptophan 2,3-dioxygenase in Tribolium castaneum. J. Insect Physiol. 107: 104-109.
Armisen, D., et al. (B.S. Oppert #43). 2018. The genome of the water strider Gerris buenoi reveals expansions of gene repertoires associated with adaptions to life on the water. BMC Genomics 19: 832.
Gomis-Gebolla, J., Y. Wang, Y. Quan, K. He; T. Walsh, B. James, S. Downes, W. Kain, P. Wang, K. Leonard, T. Morgan, B. Oppert, and J. Ferre. 2018. Analysis of cross-resistance to Vip3 proteins in eight insect colonies, from four insect species, selected for resistance to Bacillus thuringiensis insecticidal proteins. J. Invertebr. Pathol. 155: 64-70.
Schoville, S. D., et al. (Oppert, B. S. #38). 2018. A model species for agricultural pest genomics: The genome of the Colorado potato beetle, Leptinotarsa decemlineata (Coleoptera: Chrysomelidae). Sci. Rep. 8: 1931.
Silva, A. A., L. S. Braga, A. S. Correa, V. R. Holmes, J. S. Johnston, B. Oppert, R. N. C. Guedes, and M. G. Tavares. 2018. Comparative cytogenetics and derived phylogenic relationship among Sitophilus grain weevils (Coleoptera: Curculionidae: Dryophthorinae). Comparative Cytogenetics 12: 223-245.
Gundersen, D. E. et al. (S. L. Adrianos #2; B. S. Oppert #22; L. C. Perkin #24). 2017. Arthropod genomics research in the United States Department of Agriculture, Agricultural Research Service: Application of RNA interference and CRISPR gene-editing technologies in pest control. Trends in Entomology 13: 109-137.