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Brenda S Oppert

Research Molecular Biologist


Dr. Brenda Oppert                

Dr. Brenda Oppert
Research Molecular Biologist

Contact Information:

USDA-ARS-CGAHR-SPIERU
ATTN: Brenda Oppert
1515 College Avenue
Manhattan, KS  66502

brenda.oppert@ars.usda.gov

Telephone: 785.776.2780
Fax: 785.537.5584

www.ars.usda.gov/pa/cgahr/spieru/oppert


All Publications     Research Summaries     More Information     

RESEARCH INTERESTS

Dr. Oppert received B.S. and M.S. degrees in biology from the University of Texas at El Paso. She received a Ph.D. in the area of protein biochemistry from Kansas State University in 1991. Since that time, Dr. Oppert has worked at CGAHR in the area of insect gut biochemistry.
     Our research mission is to find better, safer ways to control insect pests found in and around food storage areas, including grain storage and processing facilities and warehouses. Often these pests are even smaller than field pests and provide challenges when trying to understand basic digestive processes and physiological responses. Therefore, we have adopted a number of proteomics and genomics techniques, including 2-D gel analysis, multiphase chromatography, protein identification by sequencing, peptide fingerprinting and mass spec, subgenome characterization, and gene expression. Specific research areas include the use of proteomics and genomics to understand insect responses to digestive proteinase inhibitors and microbial toxins. Comparisons of differential responses in insects have been particularly enlightening. This research will lead to improved control methods for stored product pests.


RECENT PUBLICATIONS
Adrianos, S., M. Lorenzen, and B. Oppert. 2018. Metabolic pathway interruption: CRISPR/Cas9-mediated knockout of tryptophan 2,3-dioxygenase in Tribolium castaneum. J. Insect Physiol. 107: 104-109.
Gomis-Gebolla, J., Y. Wang, Y. Quan, K. He; T. Walsh, B. James, S. Downes, W. Kain, P. Wang, K. Leonard, T. Morgan, B. Oppert, and J. Ferre. 2018. Analysis of cross-resistance to Vip3 proteins in eight insect colonies, from four insect species, selected for resistance to Bacillus thuringiensis insecticidal proteins. J. Invertebr. Pathol. 155: 64-70.
Gundersen, D. E. et al. (S. L. Adrianos #2; B. S. Oppert #22; L. C. Perkin #24). 2017. Arthropod genomics research in the United States Department of Agriculture, Agricultural Research Service: Application of RNA interference and CRISPR gene-editing technologies in pest control. Trends in Entomology 13: 109-137.
Orozco-Flores, A. A., J. A. Valadez-Lira, B. Oppert, R. Gomez-Flores, R. Tamez-Guerra, C. Rodríguez-Padilla, and P. Tamez-Guerra. 2017. Regulation by gut bacteria of immune response, Bacillus thuringiensis susceptibility and hemolin expression in Plodia interpunctella. J. Insect Physiol. 98: 275-283.
Perkin, L. C., A. R. Gerken, and B. Oppert. 2017. RNA-Seq validation of RNAi identifies additional gene connectivity in Tribolium castaneum (Coleoptera: Tenebrionidae). J. Insect Sci. 17:57; 1-7.
Perkin, L. C., E. N. Elpidina, and B. Oppert. 2017. RNA interference and dietary inhibitors induce a similar compensation response in Tribolium castaneum larvae. Insect Mol. Biol. 26: 35-45.
Tereshchenkova, V., I. Goptar, D. P. Zhuzhikov, M. A. Belozersky, Y. E. Dunaevsky, B. S. Oppert, I. Y. Filippova, and E. N. Elpidina. 2017. Prolidase is a critical enzyme for complete gliadin digestion in Tenebrio molitor larvae. Arch. Insect Biochem. Physiol. 95: e21395.
Perkin, L. C., S. L. Adrianos, and B. Oppert. 2016. Gene disruption technologies have the potential to transform stored product insect pest control. Insects 7:46; 1-15.
Perkin, L., E. N. Elpindina, and B. Oppert. 2016. Expression patterns of cysteine peptidase genes across the Tribolium castaneum life cycle provide clues to biological function. PeerJ 4: e1581.
Tereshchenkova, V. F., I. A. Goptar, I. A. Kulemzina, D. P. Zhuzhikov, M. V. Serebryakova, M. A. Belozersky, Y. E. Dunaevsky, B. Oppert, I. Y. Filippova, E. N. Elpidina. 2016. Dipeptidyl peptidase 4 – An important digestive peptidase in Tenebrio molitor larvae. Insect Biochem. Mol. Biol. 76: 38-48.