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Aspen Workman
Genetics, Breeding, and Animal Health Research
Research Microbiologist

Phone: (402) 762-4374
Fax:

STATE SPUR 18D
CLAY CENTER, NE 689330166
Projects
Tri-Partite Collaborative: Characterization of the Respiratory Microbiome and Virome Associated with Bovine Respiratory Disease Complex
Cooperative Agreement (A)
  Accession Number: 437412
Genomic Intervention Strategies to Prevent and/or Treat Respiratory Diseases of Ruminants
In-House Appropriated (D)
  Accession Number: 432111
Recent Advances in Sequencing Technologies to Characterize the Respiratory Microbiome and Virome Associated with Bovine Respiratory Disease
Interagency Reimbursable Agreement (I)
  Accession Number: 435841

Publications (Clicking on the reprint icon Reprint Icon will take you to the publication reprint.)
Host transcriptional response to persistent infection with a live-attenuated porcine reproductive and respiratory syndrome virus strain Reprint Icon - (Peer Reviewed Journal)
Chaudhari, J., Liew, C., Workman, A.M., Riethoven, J.M., Steffen, D., Sillman, S., Vu, H.L.X. 2020. Host transcriptional response to persistent infection with a live-attenuated porcine reproductive and respiratory syndrome virus strain. Viruses. 12(8):817. https://doi.org/10.3390/v12080817.
Resistance to bovine viral diarrhea virus (BVDV) infection in the bovine kidney CRIB cell line is not due to PTPN12, GRID2, or RABGAP1L mutations - (Abstract Only)
Workman, A.M., Heaton, M.P., Webster, D.A., Kalbfleisch, T.S., Smith, T.P., Carlson, D.F., Sonstegard, T.S. 2020. Resistance to bovine viral diarrhea virus (BVDV) infection in the bovine kidney CRIB cell line is not due to PTPN12, GRID2, or RABGAP1L mutations [abstract]. In: Proceedings of American Society for Virology Meeting, June 13-17, 2020, Fort Collins, Colorado. Poster No. P37-1.
Differentiation of Mannheimia haemolytica genotypes 1 and 2 by visible phenotypic characteristics on solid media - (Abstract Only)
Wynn, E.L., Schuller, G., Loy, J.D., Workman, A.M., McDaneld, T.G., Clawson, M.L. 2020. Differentiation of Mannheimia haemolytica genotypes 1 and 2 by visible phenotypic characteristics on solid media [abstract]. American Society for Microbiology Microbe, June 18-22, 2020, Chicago, Illinois. Poster No. 6696.
Differentiation of Mannheimia haemolytica genotype 1 and 2 strains by visible phenotypic characteristics on solid media Reprint Icon - (Peer Reviewed Journal)
Wynn, E.L., Schuller, G., Loy, J.D., Workman, A.M., McDaneld, T.G., Clawson, M.L. 2020. Differentiation of Mannheimia haemolytica genotype 1 and 2 strains by visible phenotypic characteristics on solid media. Journal of Microbiological Methods. 171:105877. https://doi.org/10.1016/j.mimet.2020.105877.
Upregulation of the type I interferon pathway in feedlot cattle persistently infected with bovine viral diarrhea virus Reprint Icon - (Peer Reviewed Journal)
Nilson, S.M., Workman, A.M., Sjeklocha, D., Brodersen, B., Grotelueschen, D.M., Petersen, J.L. 2020. Upregulation of the type I interferon pathway in feedlot cattle persistently infected with bovine viral diarrhea virus. Virus Research. 278:197862. https://doi.org/10.1016/j.virusres.2020.197862.
Detection of bovine viral diarrhea virus in stable flies following consumption of blood from persistently infected cattle Reprint Icon - (Peer Reviewed Journal)
Carlson, J.M., Vander Ley, B.L., Lee, S.I., Grotelueschen, D.M., Walz, P.H., Workman, A.M., Heaton, M.P., Boxler, D.J. 2020. Detection of bovine viral diarrhea virus in stable flies following consumption of blood from persistently infected cattle. Journal of Veterinary Diagnostic Investigation. 32(1):108-111. https://doi.org/10.1177/1040638719898688.
Pipelines, workflows and virtualization to build institutional informatics capacity - (Abstract Only)
Dickey, A.M., Nonneman, D.J., Freetly, H.C., Workman, A.M., Kuehn, L.A. 2019. Pipelines, workflows and virtualization to build institutional informatics capacity [abstract]. In: Proceedings of Rocky 2019, December 5-7, 2019, Aspen/Snowmass, Colorado. Paper No. P22.
Longitudinal study of humoral immunity to bovine coronavirus, virus shedding, and treatment for bovine respiratory disease in pre-weaned beef calves Reprint Icon - (Peer Reviewed Journal)
Workman, A.M., Kuehn, L.A., McDaneld, T.G., Clawson, M.L., Loy, J.D. 2019. Longitudinal study of humoral immunity to bovine coronavirus, virus shedding, and treatment for bovine respiratory disease in pre-weaned beef calves. BioMed Central (BMC) Veterinary Research. 15:161. https://doi.org/10.1186/s12917-019-1887-8.
Impact of CD46 knockout on bovine viral diarrhea virus (BVDV) replication in Madin-Darby bovine kidney cells - (Abstract Only)
A bovine CD18 signal peptide variant with increased binding activity to Mannheimia hemolytica leukotoxin Reprint Icon - (Peer Reviewed Journal)
Workman, A.M., Chitko-McKown, C.G., Smith, T.P.L., Bennett, G.L., Kalbfleisch, T.S., Basnayake, V., Heaton, M.P. 2018. A bovine CD18 signal peptide variant with increased binding activity to Mannheimia hemolytica leukotoxin. F1000Research. 7:1985. https://doi.org/10.12688/f1000research.17187.1.
Identification of BVDV2b and 2c subgenotypes in the United States: genetic and antigenic characterization Reprint Icon - (Peer Reviewed Journal)
Neill, J.D., Workman, A.M., Hesse, R., Bai, J., Poulsen-Porter, E., Meadors, B., Anderson, J., Bayles, D.O., Falkenberg, S.M. 2019. Identification of BVDV2b and 2c subgenotypes in the United States: genetic and antigenic characterization. Virology. 528:19-29. https://doi.org/10.1016/j.virol.2018.12.002.
Complete blood count data and leukocyte expression of cytokine genes and cytokine receptor genes associated with bovine respiratory disease in calves Reprint Icon - (Peer Reviewed Journal)
Lindholm-Perry, A.K., Kuehn, L.A., McDaneld, T.G., Miles, J.R., Workman, A.M., Chitko-McKown, C.G., Keele, J.W. 2018. Complete blood count data and leukocyte expression of cytokine genes and cytokine receptor genes associated with bovine respiratory disease in calves. BMC Research Notes. 11:786. https://doi.org/10.1186/s13104-018-3900-x.
First complete genome sequence of a genotype A2, subgroup 4 small ruminant lentivirus Reprint Icon - (Peer Reviewed Journal)
Workman, A.M., Clawson, M.L., Heaton, M.P., Dickey, A.M. 2018. First complete genome sequence of a genotype A2, subgroup 4 small ruminant lentivirus. MICROBIOLOGY RESOURCE ANNOUNCEMENTS. 7(19):e01337-18. https://doi.org/10.1128/MRA.01337-18.
Correlates of cross-protective immunity to porcine reproductive and respiratory syndrome virus - (Abstract Only)
Development of a multiplex real-time PCR assay using two thermocycling platforms for detection of major bacterial pathogens associated with bovine respiratory disease complex from clinical samples Reprint Icon - (Peer Reviewed Journal)
Loy, J.D., Leger, L., Workman, A.M., Clawson, M.L., Bulut, E., Wang, B. 2018. Development of a multiplex real-time PCR assay using two thermocycling platforms for detection of major bacterial pathogens associated with bovine respiratory disease complex from clinical samples. Journal of Veterinary Diagnostic Investigation. 30(6):837-847. https://doi.org/10.1177/1040638718800170.
The Wnt signaling pathway is differentially expressed during the bovine herpesvirus 1 latency-reactivation cycle: evidence that two protein kinases associated with neuronal survival (Akt3 and bone morphogenetic protein.... - (Peer Reviewed Journal)
Workman, A.M., Zhu, L., Keel, B.N., Smith, T.P.L., Jones, C. 2018. The Wnt signaling pathway is differentially expressed during the bovine herpesvirus 1 latency-reactivation cycle: evidence that two protein kinases associated with neuronal survival (Akt3 and bone morphogenetic protein receptor 2) are expressed at higher levels during latency. Journal of Virology. 92:e01937-17. https//doi.org/10.1128/JVI.01937-17.
Development of a broadly protective modified-live virus vaccine candidate against porcine reproductive and respiratory syndrome virus Reprint Icon - (Peer Reviewed Journal)
Sun, H., Workman, A., Osorio, F.A., Steffen, D., Vu, H.L.X. 2017. Development of a broadly protective modified-live virus vaccine candidate against porcine reproductive and respiratory syndrome virus. Vaccine. 36:66-73. https://doi.org/10.1016/j.vaccine.2017.11.028.
Molecular and epidemiological characterization of a respiratory disease outbreak in pre-weaned beef calves associated with bovine coronavirus - (Abstract Only)
Development and characterization of a bovine monocyte-derived macrophage cell line - (Abstract Only)
Using sheep genomes from diverse U.S. breeds to identify missense variants in genes affecting fecundity Reprint Icon - (Peer Reviewed Journal)
Heaton, M.P., Smith, T.P.L., Freking, B.A., Workman, A.M., Bennett, G.L., Carnahan, J.K., Kalbfleisch, T.S. 2017. Using sheep genomes from diverse U.S. breeds to identify missense variants in genes affecting fecundity. F1000Research. 6:1303. https://doi.org/10.12688/f1000research.12216.1.
Complete genome sequences of two genotype A2 small ruminant lentiviruses isolated from infected U.S. sheep - (Peer Reviewed Journal)
Workman, A.M., Dickey, A.M., Heaton, M.P., Clawson, M.L., Smith, T.P.L. 2017. Complete genome sequences of two genotype A2 small ruminant lentiviruses isolated from infected U.S. sheep. Genome Announcements. 5(13):e00109-17.
Potential role for a B-catenin coactivator (high mobility group AT-hook 1 protein) during the latency-reactivation cycle of bovine herpesverus 1 - (Peer Reviewed Journal)
Zhu, L., Workman, A.M., Jones, C. 2017. Potential role for a B-catenin coactivator (high mobility group AT-hook 1 protein) during the latency-reactivation cycle of bovine herpesverus 1. Journal of Virology. 91(5):e02132-16.
Evaluation of the effect of serum antibody abundance against bovine coronavirus on bovine coronavirus shedding and risk of respiratory tract disease in beef calves from birth through the first five weeks in a feedlot Reprint Icon - (Peer Reviewed Journal)
Workman, A.M., Kuehn, L.A., McDaneld, T.G., Clawson, M.L., Chitko-McKown, C.G., Loy, J.D. 2017. Evaluation of the effect of serum antibody abundance against bovine coronavirus on bovine coronavirus shedding and risk of respiratory tract disease in beef calves from birth through the first five weeks in a feedlot. American Journal of Veterinary Research. 78(9):1065-1076. https://doi.org/10.2460/ajvr.78.9.1065.
Haptoglobin concentrations in cattle either mass-medicated with gamithromycin or sham-treated - from shipping through sickness and recovery - (Abstract Only)
Chitko-McKown, C.G., Workman, A.M., DeDonder, K.D., Bennett, G.L., Apley, M.E., Harhay, G.P., Harhay, D.M., Kuehn, L.A., White, B.J., Larson, R.L., Capik, S.F., Lubbers, B.V., Clawson, M.L. 2016. Haptoglobin concentrations in cattle either mass-medicated with gamithromycin or sham-treated - from shipping through sickness and recovery [Abstract]. Conference of Research Workers in Animal Diseases, December 4-6, 2016, Chicago, IL. Abstract #135.
Haptoglobin concentrations from shipping through sickness and recovery in cattle either mass-medicated with gamithromycin or sham-treated - (Abstract Only)
Chitko-McKown, C.G., Workman, A.M., DeDonder, K.D., Bennett, G.L., Apley, M.D., Harhay, G.P., Harhay, D.M., Kuehn, L.A., White, B.J., Larson, R.L., Capik, S.F., Lubbers, B.V., Clawson, M.L. 2016. Haptoglobin concentrations from shipping through sickness and recovery in cattle either mass-medicated with gamithromycin or sham-treated. International Veterinary Immunology Symposium, August 16-19, 2016, Broadbeach, Australia. Abstract #107.
Complete genome sequence of highly virulent Porcine Reproductive and Respiratory Syndrome Virus (PRRSV) variants that recently emerged in the United States - (Peer Reviewed Journal)
Workman, A.M., Smith, T.P., Osorio, F.A., Vu, H.L. 2016. Complete genome sequence of highly virulent porcine reproductive and respiratory syndrome virus variants that recently emerged in the United States. Genome Announcements. 4(4):e00772-16.
Bovine coronavirus antibody titers at weaning negatively correlate with incidence of bovine respiratory disease in the feed yard - (Abstract Only)
Workman, A.M., McDaneld, T.G., Kuehn, L.A., Chitko-McKown, C.G., Loy, J.D. 2016. Bovine coronavirus antibody titers at weaning negatively correlate with incidence of bovine respiratory disease in the feed yard [abstract]. American Society for Virology 35th Annual Meeting, June 18-22, 2016, Blackburg, VA. Poster No. P9-9.
The differentiation and protective function of cytolytic CD4 T cells in influenza infection ()
Brown, D., Lampe, A.T., Workman, A.M. 2016. The differentiation and protective function of cytolytic CD4 T cells in influenza infection. Frontiers in Immunology. 7:93. doi:10.3389/fimmu.2016.00093
Resolving bovine viral diarrhea virus subtypes from persistently infected US beef calves with complete genome sequence - (Peer Reviewed Journal)
Workman, A.M., Heaton, M.P., Harhay, G.P., Smith, T.P.L., Grotelueschen, D.M., Sjeklocha, D.B., Brodersen, B.W., Petersen, J.L., Chitko-McKown, C.G. 2016. Resolving bovine viral diarrhea virus subtypes from persistently infected U.S. beef calves with complete genome sequence. Journal of Veterinary Diagnostic Investigation. 28(5):519-528.
ß-catenin, a transcription factor activated by canonical Wnt signaling, is expressed in sensory neurons of calves latently infected with bovine herpesvirus 1 - (Peer Reviewed Journal)
Liu, Y., Hancock, M., Workman, A.M., Doster, A., Jones, C. 2016. ß-catenin, a transcription factor activated by canonical Wnt signaling, is expressed in sensory neurons of calves latently infected with bovine herpesvirus 1. Journal of Virology. 90(6):3148-3159.
Comparison of serum PTX3 from shipping through sickness and recovery in cattle - (Abstract Only)
Chitko-McKown, C.G., Workman, A.M., DeDonder, K.D., Bennett, G.L., Apley, M.D., Harhay, G.P., Harhay, D.M., Kuehn, L.A., White, B.J., Larson, R.L., Capik, S.F., Lubbers, B.V., Clawson, M.L. 2015. Comparison of serum PTX3 from shipping through sickness and recovery in cattle [Abstract]. Conference of Research Workers in Animal Diseases, December 6-8, 2015, Chicago, IL. Abstract #070P.
Full-length coding sequence for 12 bovine viral diarrhea virus isolates from persistently infected cattle in a feedyard in Kansas - (Peer Reviewed Journal)
Workman, A.M., Harhay, G.P., Heaton, M.P., Grotelueschen, D.M., Sjeklocha, D.B., Smith, T.P.L. 2015. Full-length coding sequence for 12 bovine viral diarrhea virus isolates from persistently infected cattle in a feedyard in Kansas. Genome Announcements. 3(3):e00487-15. doi:10.1128/genomeA.00487-15.
Identification of a mannheimia haemolytica genetic subtype that causes bovine respiratory disease - (Abstract Only)
Clawson, M.L., Murray, R.W., Sweeney, M.T., Apley, M.D., DeDonder, K.D., Capik, S., Larson, R., Lubbers, B.V., Kalbfleisch, T.S., Schuller, G., Harhay, G.P., Heaton, M.P., Chitko-McKown, C.G., Workman, A.M., Harhay, D.M., Bono, J.L., Smith, T.P.L. [abstract]. 2015. Identification of a mannheimia haemolytica genetic subtype that causes bovine respiratory disease. American Society for Microbiology 2015, 115th General Meeting, May 30-June 2, 2015, New Orleans, LA. Poster #1410.
Genetic subgroup of small ruminant lentiviruses that infects sheep homozygous for TMEM154 frameshift deletion mutation A4delta53 - (Peer Reviewed Journal)
Clawson, M.L., Redden, R., Schuller, G., Heaton, M.P., Workman, A.M., Chitko-McKown, C.G., Smith, T.P.L., Leymaster, K.A. 2015. Genetic subgroup of small ruminant lentiviruses that infects sheep homozygous for TMEM154 frameshift deletion mutation A4delta53. Veterinary Research. 46:22.
Cytokine profiles from shipping through sickness and recovery in cattle either mass-medicated with gamithromycin or sham-treated - (Abstract Only)
Chitko-McKown, C.G., DeDonder, K.D., Bennett, G.L., Apley, M.D., Harhay, G.P., Kuehn, L.A., White, B.J., Larson, R.L., Capik, S.F., Lubbers, B.V., Workman, A.M. 2014. Cytokine profiles from shipping through sickness and recovery in cattle either mass-medicated with gamithromycin or sham-treated [abstract]. Conference on Research Workers in Animal Disease, December 7-9, 2014, Chicago, Illinois. Paper No. 148.