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ARS Home » Pacific West Area » Albany, California » Western Regional Research Center » Healthy Processed Foods Research » Research » Publications at this Location » Publication #404413
Research Project: Prevention of Obesity Related Metabolic Diseases by Bioactive Components of Food Processing Waste Byproducts and Mitigation of Food Allergies

Location: Healthy Processed Foods Research

Title: Milk fat globule membrane relieves fatigue via regulation of oxidative stress and gut microbiota in BALB/c mice

Author
item ZOU, XIAOXIAO - China Agricultural University
item Yokoyama, Wallace - Wally
item LIU, XIAOHUI - China Agricultural University
item WANG, KAI - China Agricultural University
item HONG, HUI - China Agricultural University
item LOU, YONGKANG - China Agricultural University
item TAN, YUQING - China Agricultural University

Submitted to: Antioxidants
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 3/9/2023
Publication Date: 3/13/2023
Citation: Zou, X., Yokoyama, W.H., Liu, X., Wang, K., Hong, H., Lou, Y., Tan, Y. 2023. Milk fat globule membrane relieves fatigue via regulation of oxidative stress and gut microbiota in BALB/c mice. Antioxidants. 12(3). Article 712. https://doi.org/10.3390/antiox12030712.
DOI: https://doi.org/10.3390/antiox12030712

Interpretive Summary: The membrane surrounding milk fat globules (MFGM) carries proteins necessary for the health of the newborn. In this study we evaluated the antifatigue and antioxidant potential of MFGM fed to mice subjected to the forced swimming test. We found that MFGM extended the time to exhaustion and also reduced antioxidant markers in the blood. MFGM also altered the composition of the gut microbiome that may have benefits to the host. Our results suggested the potential application of MFGM as an antifatigue and antioxidant dietary supplements.

Technical Abstract: Milk fat globule membranes (MFGM) are complex structures incorporating bioactive proteins and lipids to assist infant development. However, the antifatigue and antioxidant potential of MFGM have not been investigated. Repeated force swimming measured fatigue in male BALB/c mice fed MFGM and saline for 18 weeks. MFGM supplementation increased the time to exhaustion, 42.7% at 6 weeks and 30.6% at 14 weeks. Fatigue and injury-related biomarkers, including blood 18 glucose, lactic acid, and lactate dehydrogenase, were ameliorated. The activity of antioxidant enzymes in serum increased at 18 weeks, while malondialdehyde (MDA) content decreased by 45.0% 20 after MFGM supplementation. Pearson correlation analysis showed a high correlation between fatigue-related indices and antioxidant levels. The increased protein expression of hepatic Nrf2 reduced the protein expression of Caspase-3 in the gastrocnemius muscle. Moreover, MFGM supplementation increased the relative abundance of Bacteroides, Butyricimonas, and Anaerostipes. Our results suggested the potential application of MFGM as an antifatigue and antioxidant dietary supplements.