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ARS Home » Southeast Area » New Orleans, Louisiana » Southern Regional Research Center » Commodity Utilization Research » Research » Publications at this Location » Publication #394364
Research Project: Development of Novel Cottonseed Products and Processes

Location: Commodity Utilization Research

Title: Real-time quantitative PCR analysis of gene expression in mouse macrophages treated with cottonseed-derived gossypol

Author
item Cao, Heping

Submitted to: NIH Gene Expression Omnibus (GEO) Database
Publication Type: Database / Dataset
Publication Acceptance Date: 5/15/2022
Publication Date: 5/15/2022
Citation: Cao, H. 2022. Real-time quantitative PCR analysis of gene expression in mouse macrophages treated with cottonseed-derived gossypol. NIH Gene Expression Omnibus (GEO) Database. GSE203027.

Interpretive Summary: Plant bioactive extracts have been used for disease prevention and treatment since ancient history. One of the major bioactive compounds in plant extracts are plant polyphenols. Gossypol is a complex plant polyphenol from cotton plants especially in the glanded cottonseed. Long-term consumption of cottonseed oil with high concentration of gossypol contributes to its toxicity resulting in male infertility. However, recent studies have demonstrated that gossypol and related compounds have anticancer and anti-inflammatory activities, but molecular evidence is not substantial. The objective of this study was to explore the molecular effects of gossypol on the expression of 27 genes involved in inflammatory responses, lipid metabolism, glucose transport and insulin signaling pathway in mouse macrophages. This technical note describes the dataset of relative quantitation of 27 mRNAs in mouse RAW264.7 macrophages generated by the SYBR Green qPCR method.

Technical Abstract: Gossypol is a complex plant polyphenol reported to be cytotoxic and anti-inflammatory, but little was known about its effect on gene expression in macrophages. The objective was to explore the regulation of gossypol on gene expression involved in inflammatory responses, lipid biosynthesis, glucose transport and insulin signaling pathway in mouse macrophages. Mouse RAW264.7 macrophages were treated with multiple concentrations of gossypol for 2-24 h. qPCR analyzed the expression of 27 genes coding for anti-inflammatory tristetraprolin family (TTP/ZFP36), proinflammatory cytokine, lipid biosynthesis (DGAT), glucose transporter (GLUT) and insulin signaling genes. This technical note described the dataset of relative quantitation of 27 mRNAs in mouse RAW264.7 macrophages generated by the SYBR Green qPCR method.