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Research Project: Prevention of Obesity Related Metabolic Diseases by Bioactive Components of Food Processing Waste Byproducts and Mitigation of Food Allergies

Location: Healthy Processed Foods Research

Title: Effects of kefir lactic acid bacteria-derived postbiotic components on high fat diet-induced gut microbiota and obesity

item SEO, KUN-HO - Konkuk University
item LEE, HYEON GYU - Konkuk University
item EOR, JU YOUNG - Konkuk University
item JEON, HYE JIN - Hanyang University
item Yokoyama, Wallace - Wally
item KIM, HYUNSOOK - Hanyang University

Submitted to: Food Research International
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/27/2022
Publication Date: 5/30/2022
Citation: Seo, K., Lee, H., Eor, J., Jeon, H., Yokoyama, W.H., Kim, H. 2022. Effects of kefir lactic acid bacteria-derived postbiotic components on high fat diet-induced gut microbiota and obesity. Food Research International. 157. Article 111445.

Interpretive Summary: The gut colonization of bacteria beneficial to health or probiotics was thought to be necessary for their benefit. In this study the cell wall proteins of a lactic bacteria was isolated and fed to mice on high fat diets. The bacterial protein isolates were able to reduce weight gain, fat weight and improved insulin sensitivity. The protein also reduced inflammatory genes and increased genes related to immune response.

Technical Abstract: Cellular components, surface layer protein (SLP) and exopolysaccharides (EPS) of postbiotic lactic bacteria (PLAB) can rehabilitate high-fat diet-induced dysbiosis in obese gut microbiome. However, is not clear whether and how postbiotic components affect gut microbiota and profiling of adipocyte gene expression. Furthermore, the cellular components of PLAB in combination with prebiotic wine grape seed flour (GSF) may have greater benefit on high-fat diet (HFD)-induced obesity and gut microbiota imbalance. To investigate interactions, C57BL/6 mice were fed a HFD with orally administered saline (CON), EPS, or SLP or saline fed 2% GSF (GSF) or combination (EPS+SLP+GSF; ALL). There were significant reductions of HFD-induced body weight gain, adipose weight, serum triglyceride, and insulin resistance by the SLP and ALL diets compared to CON, with the most profound effect by ALL. ALL significantly affected the distribution of intestinal bacterial genus and species particularly those involved in production of short chain fatty acid (SCFA) and anti-obesogenic action. Microarray analysis from adipose tissue showed that ALL significantly affected expression of genes related to fatty acid biosynthesis, autophagy, inflammatory response, immune response, brown adipose tissue development and response to lipoteichoic acid and peptidoglycan (p < 0.05). Interestingly, expression of Akp13 (A-kinase anchoring protein 13) gene, which is related to body mass index and immune response, was negatively associated with the abundance of obesogenic and SCFAs producing gut bacteria. These data suggest that a combination of postbiotic kefir LAB cellular components and prebiotic GSF establishes a healthy intestinal microbiota that in part was associated with the prevention of obesity and obesity-related diseases.