Cottonseed kernel extracts downregulate proinflammatory cytokine gene expression in mouse macrophages

Authors: Cao, Heping; Sethumadhavan, Kandan

Submitted to: American Chemical Society National Meeting
Publication Type: Abstract Only
Publication Acceptance Date: 5/26/2022
Publication Date: N/A
Citation: N/A

Interpretive Summary:

Technical Abstract: Gossypium hirsutum L. (Cotton) produces high-value fiber and low-value cottonseed. Cottonseed is classified as either glanded or glandless seed depending on the presence or absence of pigmented gossypol glands in the seed. Cottonseed value may be increased by providing bioactive compounds with health promotion and disease prevention potentials. Cottonseed ethanol extracts were isolated from both glanded and glandless seed and analyzed by HPLC-MS to be essentially free of toxic gossypol. The objective of this study was to investigate if cottonseed extracts reduce proinflammatory cytokine gene expression in mouse macrophages. Mouse RAW264.7 macrophages were treated with various concentrations of cottonseed extracts for 2, 8 and 24 h. qPCR was used to analyze the expression of several proinflammatory genes coding for tumor necrosis factor (TNF), prostaglandin-endoperoxide synthase 2 (PGS2/COX2), granulocyte-macrophage colony stimulating factor (GM-CSF), interferon gamma (IFN'), interleukin (IL) 6 and IL12b cytokines. Glanded cottonseed kernel extract reduced all but TNF mRNA levels in mouse macrophages. Glandless cottonseed kernel extract decreased the expression of all five proinflammatory cytokine genes in the cells. However, cottonseed coat extracts did not exhibit apparent effects on decreasing mRNA levels of the poinflammatory cytokine genes in the macrophages. We propose that cottonseed kernel extracts possesses anti-inflammatory properties.