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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Characterization and Interventions for Foodborne Pathogens » Research » Publications at this Location » Publication #385496

Research Project: Development of Innovative Technologies and Strategies to Mitigate Biological, Chemical, Physical, and Environmental Threats to Food Safety

Location: Characterization and Interventions for Foodborne Pathogens

Title: Application of bacteriophage in rapid detection of Escherichia coli in foods

item WANG, JUN - Qingdao Agricultural University
item KANACH, ANDREW - Purdue University
item HAN, RONGWEI - Purdue University
item APPLEGATE, BRUCE - Purdue University

Submitted to: Current Opinion in Food Science
Publication Type: Review Article
Publication Acceptance Date: 12/1/2020
Publication Date: 6/1/2021
Citation: Wang, J., Kanach, A., Han, R., Applegate, B. 2021. Application of bacteriophage in rapid detection of Escherichia coli in foods. Current Opinion in Food Science. 39:43–50.

Interpretive Summary:

Technical Abstract: Food is a common vehicle for introducing Escherichia coli into humans which can result in serious disease. Efficient and rapid detection of E. coli in the multitude of food and beverage matrices available is critical to limit the health, financial, and social impacts of these infections. Compared to culture-based and molecular detection methods, bacteriophage-based detection methods perform favorably with high specificity, natural affinity, low cost, and reliable live-dead cell distinction. More recently, many rapid bacteriophage-based detection approaches have been developed coupled with advanced techniques including fluorescence, immunology, molecular biology, nanoscale science, and an array of biosensing strategies. In this review, up-to-date applications and principles of bacteriophage-based detection methods for E. coli in food are discussed, in addition to their current limitations and future prospects.