|NERY DA SILVA, MARINA - Sao Paulo State University (UNESP)|
|FERRIERA, PAULA - Sao Paulo State University (UNESP)|
|GONCALVES, DANIELLE - Sao Paulo State University (UNESP)|
|SPOLIDORIO, LUIS - Sao Paulo State University (UNESP)|
|CESAR, THAIS - Sao Paulo State University (UNESP)|
Submitted to: Journal of Food Science and Nutrition
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 1/17/2021
Publication Date: 2/12/2021
Citation: Nery Da Silva, M., Ferriera, P.S., Goncalves, D.R., Spolidorio, L.C., Manthey, J.A., Cesar, T.B. 2021. Tangeretin and heptamethoxyflavone decrease insulin resistance, fat accumulation and oxidative stress in a study of C57BL/6J mice fed a high-fat diet. Journal of Food Science and Nutrition. https://doi.org/10.1002/fsn3.2167.
Interpretive Summary: Two compounds in oranges were tested in mice to see if they could alleviate the health problems associated with high fat diets. The problems are disturbances to the health of livers, elevated glucose levels, and elevated levels of internal inflammation. The results showed a moderate level of protection of the liver, mild decreases of blood glucose, and mildly decreased internal inflammation. There were interesting differences in the effects caused by these two individual compounds, and this shows that there are important features of the roles of these compounds that we still don't understand, but this study continues to show that citrus is a healthy fruit to eat.
Technical Abstract: Tangeretin (TAN) and heptamethoxyflavone (HMF) were investigated for their abilities to repair metabolic damages caused by a high-fat diet (HFD) in C57BL/6J mice. In the first four weeks, mice were fed either a standard diet (11% kcal from fat), or a HFD (45% kcal from fat). In the following four weeks, two groups received HFDs supplemented with either TAN or HMF at doses of 100 mg/kg body weight, while the two remaining groups continued to receive a standard healthy diet or a non-supplemented HFD. TAN and HMF decreased blood serum glucose, leptin and resistin, and lowered the insulin resistance in mice fed the HFD. Blood serum peroxidation (TBARS) levels were lower in the TAN and HMF supplemented groups than in the non-supplemented HFD group. TAN reduced adipocyte size, while HMF decreased fat accumulation in the liver. A significant elevation of the anti-inflammatory cytokine, IL-10, occurred in the HMF group compared to the HFD and control groups. The results showed that TAN and HMF, and their metabolites, prevent metabolic alterations associated with the development of metabolic syndrome and type 2 diabetes, and also, there appear to be differences in the effects of HMF and TAN supplementation. Several main classes of metabolites occurred as a result of the oral doses of TAN and HMF, and with HMF, a new set of methylglucuronide conjugates were detected. Differences among the numbers and the structures of TAN and HMF metabolites may attribute to the differences in effects produced by the dosing with these two compounds.