Immunoassay biosensing of foodborne pathogen with surface plasmon resonance imaging: A review

Authors: WANG, BIN - Oak Ridge Institute For Science And Education (ORISE); Park, Bosoon

Submitted to: Journal of Agricultural and Food Chemistry
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 8/20/2020
Publication Date: 8/20/2020
Citation: Wang, B., Park, B. 2020. Immunoassay biosensing of foodborne pathogen with surface plasmon resonance imaging: A review. Journal of Agricultural and Food Chemistry. https://dx.doi.org/10.1021/acs.jafc.0c02295.
DOI: https://doi.org/10.1021/acs.jafc.0c02295

Interpretive Summary: This paper introduces one of emerging optical technology, surface plasmon resonance imaging (SPRi) for pathogen detection in food samples using different types of SPRi biosensors based on antibody-antigen binding. Nowadays SPRi biosensors are widely researched for detection of toxins of organic molecules, proteins, and bacteria. The major advantage of SPRi includes rapid detection without sample labelling process and identification of multiple targets from mixture sample on one biochip at the same time. In addition, SPRi biosensors can be used for small bioanalytical instruments that are easy to move and operate in field detections. Most studies reviewed in this paper have focused on how to increase specificity and sensitivity of SPRi signals on sensor chip, in order to detect target pathogens at very low concentrations without interruption of other biological materials in the same sample. Finally, this paper discussed major challenges what researchers encounter when detecting multiple pathogens in real-world food samples, and the potential nanotechnologies to reduce the size and complexity of SPRi sensors in the future.

Technical Abstract: This review introduces the fundamental principles and major instrumental configurations of surface plasmon resonance imaging (SPRi), and summarizes the recent advances in immunoassay biosensing using SPRi techniques with special emphasis on the multiplex detections of foodborne pathogens. SPRi have been increasingly used in the label-free detections of various biospecies such as organic toxins, proteins, and bacteria, with the advantages of rapid detection in tens of minutes and multiplex detection of multiple targets with a same biochip. Both prism-based and prism-free configurations of SPRi have been developed for highly integrated portable immunosensors, which have shown the great potential for pathogen detection and living cell imaging. Combined with a method of microarray immunoassay, various spotting techniques, surface modification protocols, and signal amplification approaches have been developed to improve sensitivity and specificity of SPRi biochip. The challenges in multiplex detections of foodborne pathogens in real-world samples are addressed, and future perspectives of miniaturizing SPRi immunosensors with nanotechnologies are discussed.