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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Molecular Characterization of Foodborne Pathogens Research » Research » Publications at this Location » Publication #369247

Research Project: Development of Portable Detection and Quantification Technologies for Foodborne Pathogens

Location: Molecular Characterization of Foodborne Pathogens Research

Title: Complete genome sequence and annotation of Campylobacter jejuni YH003 isolated from retail chicken

Author
item He, Yiping
item Reed, Sue
item Strobaugh, Terence

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 12/26/2019
Publication Date: 1/23/2020
Citation: He, Y., Reed, S.A., Strobaugh Jr, T.P. 2020. Complete genome sequence and annotation of Campylobacter jejuni YH003 isolated from retail chicken. Microbiology Resource Announcements. https://doi.org/10.1128/MRA.01307-19.
DOI: https://doi.org/10.1128/MRA.01307-19

Interpretive Summary: Campylobacter is a common cause of bacterial foodborne illness worldwide. Within 26 species and 9 subspecies of the Campylobacter genus, C. jejuni is responsible for over 90% cases of these infection. This pathogen can be commonly found in chicken and other meat products. In this study, we isolated a new C. jejuni strain YH003 from retail chicken. By combining the big data generated from PacBio single molecular real-time and Illumina MiSeq sequencing technologies, we were able to assemble a complete genome sequence of the strain. Bioinformatic analysis of the genome revealed an abundance of virulence and antibiotic resistance genes in the strain, indicating a high potential for this organism to cause disease. This whole genome sequencing study of a C. jejuni food isolate has provided valuable genetic information, particularly the virulence determinants of the organism.

Technical Abstract: Campylobacter jejuni strain YH003, isolated from retail chicken, was sequenced using PacBio and Illumina technologies. A complete genome was assembled and annotated. The genome is 1,743,985 base pairs with 30.3% G+C content. It contains a number of virulence and antibiotic resistance genes, including a type VI secretion system, cytolethal distending toxin gene cluster cdtCBA and multidrug efflux system of cmeABCR.