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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Molecular Characterization of Foodborne Pathogens Research » Research » Publications at this Location » Publication #359149

Research Project: Development of Portable Detection and Quantification Technologies for Foodborne Pathogens

Location: Molecular Characterization of Foodborne Pathogens Research

Title: Pulsed-field gel electrophoresis typing of Staphylococcus aureus strains

Author
item He, Yiping
item Reed, Sue

Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 7/13/2019
Publication Date: 9/16/2019
Citation: He, Y., Reed, S.A. 2019. Pulsed-field gel electrophoresis typing of Staphylococcus aureus strains. Methods in Molecular Biology. New York, New York: Springer. 2069:78-88. https://doi.org/10.1007/978-1-4939-9849-4_5.
DOI: https://doi.org/10.1007/978-1-4939-9849-4_5

Interpretive Summary:

Technical Abstract: Pulse field gel electrophoresis (PFGE) has been routinely used in food testing and clinical laboratories for molecular typing of bacterial strains and outbreak investigation. In this book chapter, we updated the PFGE protocols used for typing Staphylococcus aureus, a common clinical and foodborne pathogen which can cause various diseases ranging from skin and tissue infections, toxin-mediated diseases, pneumonia, and bacteremia. The method described here can be adopted for genotyping other bacterial strains. The streamlined experimental conditions and procedures could facilitate the applications of this technique in pathogen differentiation and epidemiological investigation.