Location: Molecular Characterization of Foodborne Pathogens ResearchTitle: Pulsed-field gel electrophoresis typing of Staphylococcus aureus strains
Submitted to: Methods in Molecular Biology
Publication Type: Book / Chapter
Publication Acceptance Date: 7/13/2019
Publication Date: 9/16/2019
Citation: He, Y., Reed, S.A. 2019. Pulsed-field gel electrophoresis typing of Staphylococcus aureus strains. In: Walker, J.M., editor. Methods in Molecular Biology. New York, NY: Springer. 2069:78-88. https://doi.org/10.1007/978-1-4939-9849-4_5.
Technical Abstract: Pulse field gel electrophoresis (PFGE) has been routinely used in food testing and clinical laboratories for molecular typing of bacterial strains and outbreak investigation. In this book chapter, we updated the PFGE protocols used for typing Staphylococcus aureus, a common clinical and foodborne pathogen which can cause various diseases ranging from skin and tissue infections, toxin-mediated diseases, pneumonia, and bacteremia. The method described here can be adopted for genotyping other bacterial strains. The streamlined experimental conditions and procedures could facilitate the applications of this technique in pathogen differentiation and epidemiological investigation.