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Research Project: Programmed Assembly of Cell Based Carriers for Improved Functionality of Bioactives in the Gut

Location: Healthy Processed Foods Research

Title: Characterizations on the stability and release properties of ß ionone loaded thermosensitive liposomes (TSLs)

item CHEN, LING - Jiangnan University
item LIANG, RONG - Jiangnan University
item WANG, YIHAN - Jiangnan University
item Yokoyama, Wallace - Wally
item CHEN, MAOSHEN - Jiangnan University

Submitted to: Journal of Agriculture and Food Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 5/30/2018
Publication Date: 5/30/2018
Citation: Chen, L., Liang, R., Wang, Y., Yokoyama, W.H., Chen, M. 2018. Characterizations on the stability and release properties of ß ionone loaded thermosensitive liposomes (TSLs). Journal of Agriculture and Food Sciences. 66(31):8336-8345.

Interpretive Summary: Cancer cells have a higher temperature than normal cells. Liposomes were developed that release their contents at the temperature of cancer cells and may release their contents in proximity to cancer but not normal cells. The experiment show that B-Ionone, a natural anticancer agent, was released at the higher temperature of cancer cells.

Technical Abstract: Liposomes with phase transition temperatures, Tm, near pathogenic site temperature are potential chemoprophylactic delivery vehicles. We prepared and characterized the thermal properties of liposomes composed of 1,2- dipalmitoyl-sn-glycero-3-phosphocholine (DPPC) and hydrogenated soy phosphatidylcholine (HSPC) incorporating ß-ionone with Tm at 42 °C. Liposomes with ß-ionone/lipid ratio (w/w) of 1:20 and 1:8 had the necessary stability and released most of the ß-ionone. The molecular architecture surrounding Tm was studied by fluorescent probes, Raman spectroscopy, and differential scanning calorimeter (DSC). ß-Ionone was found to be preferentially located in the deep regions of the lipid bilayer (toward the long chain alkyl of the lipid) at moderate loading. The results showed that ß-ionone encapsulated liposomes have a superior release at higher loading amount. Increasing ß-ionone leads to disorder in the liquid crystalline state and accelerates the release rate. These studies provide information on the membrane structural properties of ß-ionone loaded liposomes that guide rational bioactive molecular delivery systems design for health products.