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ARS Home » Plains Area » Manhattan, Kansas » Center for Grain and Animal Health Research » Stored Product Insect and Engineering Research » Research » Publications at this Location » Publication #355349

Research Project: Sustainable Management Strategies for Stored-Product Insects

Location: Stored Product Insect and Engineering Research

Title: Draft genome sequence of an Erwinia tracheiphila isolate from an infected muskmelon (Cucumis melo)

item SHAPIRO, LORI - Harvard Medical School
item ANDRADE, ANDRES - Harvard Medical School
item Scully, Erin
item ROCHA, JORGE - Centro De Investigacion En Alimentaction Y Desarollo
item PAULSON, JOSEPH - Genentech
item KOLTER, ROBERTO - Harvard Medical School

Submitted to: Microbiology Resource Announcements
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 10/4/2018
Publication Date: 11/1/2018
Citation: Shapiro, L.R., Andrade, A., Scully, E.D., Rocha, J., Paulson, J.N., Kolter, R. 2018. Draft genome sequence of an Erwinia tracheiphila isolate from an infected muskmelon (Cucumis melo). Microbiology Resource Announcements. 7(17):e01058-18.

Interpretive Summary: Erwinia tracheiphila is a bacterial pathogen that is known to infect plants in two groups (genera); one that contains muskmelons and cucumbers, and another that contains pumpkins, squash, and yellow-flowered gourds. Recent analyses have demonstrated that there are three major groups of this pathogen that occur in North America. One group (Et-melo) has a broad geographic distribution encompassing the Northeastern and Midwestern United States, but can only cause infections in group with muskmelons and cucumbers. The other two groups, Et-C1 and Et-C2, have less extensive geographic distributions and occur only in the Northeastern United States, but can infect both plant groups. The genome of a member of the Et-C2 group was previously sequenced, but no sequences from the other two groups were available. In this study, we sequenced the genome of a representative of the Et-melo group. Comparisons between the Et-melo and Et-C2 genomes can lead to the identification of genes responsible for determining host range and may allow us to identify specific factors that enable infection of specific plants.

Technical Abstract: Erwinia tracheiphila is a bacterial plant pathogen emerging in temperate Eastern North America. To provide a starting point for understanding genetic variation within this species, here we sequence isolate MDCuke, the first high quality draft E. tracheiphila reference genome from an infected muskmelon (Cucumis melo). The genome assembles into a single chromosomal contig with three plasmid contigs and one bacteriophage contig.