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ARS Home » Northeast Area » Wyndmoor, Pennsylvania » Eastern Regional Research Center » Molecular Characterization of Foodborne Pathogens Research » Research » Publications at this Location » Publication #354323

Research Project: Development of Portable Detection and Quantification Technologies for Foodborne Pathogens

Location: Molecular Characterization of Foodborne Pathogens Research

Title: Whole genome sequencing and annotation of Staphylococcus aureus strain SJTUF_J27 isolated from seaweed

Author
item XIE, YANPING - Former ARS Employee
item He, Yiping
item GHATAK, SANDEEP - Indian Council Of Agricultural Research (ICAR)
item Irwin, Peter
item Yan, Xianghe
item Strobaugh, Terence
item Gehring, Andrew

Submitted to: Data in Brief
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 9/1/2018
Publication Date: 10/1/2018
Citation: Xie, Y., He, Y., Ghatak, S., Irwin, P.L., Yan, X., Strobaugh Jr, T.P., Gehring, A.G. 2018. Whole genome sequencing and annotation of Staphylococcus aureus strain SJTUF_J27 isolated from seaweed. Data in Brief. 20:894-898. https://doi.org/10.1016/j.dib.2018.08.084.
DOI: https://doi.org/10.1016/j.dib.2018.08.084

Interpretive Summary: Staphylococcus aureus is one of the most common causes of bacterial foodborne illness in the United States. Staphylococcal food poisoning typically results from ingesting enterotoxins in food products contaminated with S. aureus. By using Illumina MiSeq sequencing technology, we completed the whole genome sequence of S. aureus strain SJTUF_J27 isolated from seaweed in China. Bioinformatic analysis of the genome revealed an abundance of virulence genes including various antimicrobial resistance genes. The genetic information revealed in this work provides insight into the antibiotic resistance and virulence potential of this organism

Technical Abstract: Staphylococcus aureus produces a wide variety of enterotoxins which can cause food poisoning in humans. Herein we report the complete genome sequence of S. aureus SJTUF_J27 isolated from seaweed in China. The size of the genome is 2.80 Mbp with 32.9% G+C content, consisting of 2614 coding sequences and 77 RNAs. A number of virulence factors, including antimicrobial resistance genes (fluoroquinolone, beta-lactams, fosfomycin, mupirocin, trimethoprim, and aminocoumarin) and the egc enterotoxin cluster, were found in the genome. In addition, the genes encoding metal-binding proteins and associated heavy metal resistance were identified. Phylogenetic analysis, based upon genome-wide single nucleotide polymorphisms (SNPs), and BLAST Ring Image Generator (BRIG) evaluation were performed for SJTUF_J27 and four S. aureus strains isolated from food. The completed genome sequence was deposited in NCBI’s GenBank under the accession number CP019117.