Comparative proteomic profiling between each of two consecutive developmental stages of the Solanum fruit fly, Bactrocera latifrons (Hendel)

Authors: Chang, Chiou; Geib, Scott

Submitted to: International Journal of Molecular Sciences
Publication Type: Peer Reviewed Journal
Publication Acceptance Date: 7/2/2018
Publication Date: 7/9/2018
Citation: Chang, C.L., Geib, S.M. 2018. Comparative proteomic profiling between each two consecutive developmental stages of the Solanum fruit fly, Bactrocera latifrons (Hendel). International Journal of Molecular Sciences. doi:10.3390/ijms19071996.
DOI: https://doi.org/10.3390/ijms19071996

Interpretive Summary: The Solanum fruit fly, Bactrocera latifrons (Hendel), as a complex and dynamic holometabolous insect, goes through many forms (egg, larva, pupa, and adult) during their development. Understanding of “what”, “when”, “where”, “why”, and “how” many hundred thousand proteins exist in an insect, interact and express during fruit fly development at molecular level not only can expand the knowledge base, but also lead to the development of novel fruit fly control techniques. Here, we took comparative proteomics approach and established a proteome profiling across development between 2 consecutive stages of the solanum fruit fly, Bactrocera latifrons (Hendel), using 2-D gel electrophoresis and mass spectrometry. Samples of 3-d-old eggs, 1 and 10-d-old larvae, 1 and 10-d-old pupae, 1 and 9-d-old females and males of B. latifrons were used. A custom peptide database, derived from the de novo B. latifrons whole genome assembly was used for peptide identification. Differentially expressed proteins and functions between 2 consecutive developmental stages were identified, annotated, and listed in gel images and/or charts. With this foundational information on hands, any epigenetic impacts due to the abiotic or biotic environmental factors can be easily identified, manipulated, and further led to gene editing research.

Technical Abstract: Samples of 1 and 10 days old larvae, 1 and 10 days old pupae, 1 and 9 days old females and males, and 3 days old eggs of B. latifrons were collected and analyzed using 2-D gel electrophoresis and mass spectrometry. A custom peptide database, derived from the de novo B. latifrons whole genome assembly was adopted for peptide identification. Differentially expressed proteins were identified in each stage, using the equivalents first day of the stage as the comparison and putative protein function was annotated in representative SDS gel images, charts, and tables.